The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effects of Loranthus parasiticus Merr. on Cell Cycle and Expression of Related Genes in HepG2 Cell

상기생(桑寄生)이 HepG2 cell의 세포분열 및 관련유전자 발현에 미치는 영향

  • Rhew, Kwang-Yul (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Young-Chul (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Woo, Hong-Jung (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Jang-Hoon (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University)
  • 류광열 (경희대학교 한의과대학 간계내과학교실) ;
  • 김영철 (경희대학교 한의과대학 간계내과학교실) ;
  • 우홍정 (경희대학교 한의과대학 간계내과학교실) ;
  • 이장훈 (경희대학교 한의과대학 간계내과학교실)
  • Published : 2005.03.30
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Abstract

Objectives : The aim of this study was to evaluate the effects of Loranthus parasiticus Merr. on cell cycle and expression of related genes in HepG2 cells. Methods : The MTT assay, cell counting assay, $[^3H]-Thymidine$ incorporation assay, flow cytometric analysis, quantitative RT-PCR and western blot assay were studied. Results : In the water extract of Loranthus parasiticus Merr., inhibition of cell proliferation and DNA synthesis in HepG2 cells was seen. These inhibitory effects were due to inhibition of G l-S transition in cell cycle. After treatment with the extract, expression of cyclin D1(G1 check point related gene) was inhibited particularly in dose-dependent and time-dependent manners. Conclusion : These results suggest that the inhibition of cell cycle progression by Loranthus parasiticus Merr. in HepG2 cell is due to suppression of cyclin D1(G1 check point related gene) mRNA expression and protein synthesis.

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