The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Effect of Gleditsiae Spina extract on immune cells and cytokines in OVA-induced asthmatic mice

급각자가 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향

  • Song, Sang-Jin (Division of Respiratory System, Dep. of Internal Medicine College of Oriental Medicine, Daejeon University) ;
  • Park, Yang-Chun (Division of Respiratory System, Dep. of Internal Medicine College of Oriental Medicine, Daejeon University)
  • 송상진 (대전대학교 한의과대학 폐계내과학교실) ;
  • 박양춘 (대전대학교 한의과대학 폐계내과학교실)
  • Published : 2005.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : The purpose of this research is to examine the effects of Gleditsiae Spina (GS) extract on immune cells and cytokines in ovalbumin (OVA)-induced asthmatic mice. Methods : In vivo, C57BL/6 mice were sensitized and handicapped by OVA for 12 weeks. During this experiment, the one group was then treated with GS extract for the later 8 weeks (3 times per week) and analyzed by ELISA, flow cytometer and RT-PCR. Results : In vivo, there were significant decreases in eosinophils, IL-4, IL-5, IL-13, IgE in BALF (bronchoalveolar lavage fluid). However, $IFN-{\gamma}$ in BALF of GS group increased significantly, compared with that of control group. Additionally, the population of $CD3e^-/CCR3^+,\;CD69^+/CD3e^+,\;IgE^+/B220^+,\;CD11b^+/Gr-l^+$ cells in the GS group decreased. Conclusion : The results of this study support a role for GS as an effective treatment for asthma in its experimental success in significantly decreasing inflammation and asthma reactions, and in increasing $IFN-{\gamma}$, which helps prevent such reactions.

Keywords