Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Inhibitory Effect of Euscaphic Acid and Tormentic Acid from the Roots of Rosa rugosa on High Fat Diet-Induced Obesity in the Rat

고지방 식이로 유도된 비만 흰쥐에서 해당근에서 분리된 Euscaphic Acid 및 Tormentic Acid의 효과

  • Published : 2005.12.30
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Abstract

The roots of Rosa rugosa have been used to treat diabetes mellitus in the folkloric society of Korea. To demonstrate the active component for the rat obesity induced by high fat diet for 6 weeks, the phytochemical fractionation and the pharmacological activity test were performed on this crude drug. It was shown that the methanolic extract and its EtOAc fraction inhibited the weight increase of the rat body, abdominal fat pad and hyperlipidemia at 200 mg/kg dose. Further, the triterpenoids, euscaphic acid and tormentic acid, isolated from R. rugosa roots were active at 30 mg/kg in the same assay. The two components shifted serum total-, HDL, and LDL-cholesterol levels toward the values of the unteated group, suggesting that the active compounds has hypolipidemic effects. The rats fad euscaphic acid and tormentic acid also reduced thiobarbituric acid-reactive substance (TBARS) and hydroxyl radical in the rat blood and increased superoxide dismutase activity compared to the control. TBARS values and carbonyl contest of the hepatic protein were reduced by treatment with the two triterpenoids. Antioxidative enzyme (SOD, glutathione peroxidase, and catalase) activities in hepatic were increased by treatment of rats with the triterpenoids, which suggests that triterpenoids inhibited the reduction of hepatic antioxidative activity caused by high fat diet. Taken together, these results support that euscaphic acid and tormentic acid improve a high fat diet-induced hyperlipidemia via the activation of antioxidative mechanism.

Keywords

References

  1. Rees, K. R. (1964) Fatty acid induction by toxic agent (inmetabolism and physiological significance oflipid), 443-453, John Wiley & Son Ltd
  2. Gordis, E. (1969) Lipid etabolism of carbon tetrachloride, J. Clin. Invest., 48: 203-209 https://doi.org/10.1172/JCI105969
  3. Redgraviln, T. G and Zilversmit, B. B. (1969) Does puromycin block release of chylomicrin from the intestine, Amer. J. Physiol., 217: 336-340
  4. Young, H. S., Park J. C., and Choi, J. S. (1987) Triterpenoid glycosides from Rosa rugosa, Arch. Pharm. Res., 10: 219-222 https://doi.org/10.1007/BF02857743
  5. Young, H. S., Park, J. C., and Choi, J. S. (1987) Isolation of (+)-catechin from the roots of Rosa rugosa, Kor. J Pharmacogn., 18: 177-179
  6. Murakami, C., Ishijima, K., Hirota, M., Sakaguchi, K., Yoshida, H., and Mizushina, Y. (2002) Novel anti-inflammatory compounds from Rubus sieboldii, triterpenoids, are inhibitors of mammalian DNA polymerases, Biochimica et Biophysica Acta, 1596: 193-200 https://doi.org/10.1016/S0167-4838(02)00227-3
  7. Jung, H. J., Nam, J. H., Coi, J., Lee, K. T., and Park, H. J. (2005) $19\alpha$-Hydroxyursane-type triterpenoids: antinociceptive antinflammatory princicples ofthe roots of Rosa rugosa, Biological and Pharmaceutical Bulletin, 28: 101-104 https://doi.org/10.1248/bpb.28.101
  8. Richmond, W. (1976) Use of cholesterol oxidase for assay of total and free cholesterol in serum by continuous flow analysis, Clin. Chem., 22: 1579-1588
  9. Ajit, S., Joseph, D., and Bennie, Z. (1987) A method for the sequential colorimetric determination of serum triglycerides and cholesterol, Clin. Biochem., 20: 167-172 https://doi.org/10.1016/S0009-9120(87)80115-7
  10. Ven Gent, c.M., and Roseleur, OJ. (1974) Quantitative determination ofphospholipids in blood serum or plasma by a nondestructive method, Clin. Chim. Acta, 57: 197-203 https://doi.org/10.1016/0009-8981(74)90397-0
  11. Kobatake, Y., Saito, M., Kuroda, K. Kobayashi, S., and Innami, S. (1987) Influence of fish consumption on serum lipid and lipid peroxide concentrations in middle aged subjects. J. Japan Soc. Nutr & Food Sci., 40: 103-107 https://doi.org/10.4327/jsnfs.40.103
  12. Oyanagui, Y. (1984) Reevaluation of assay methods and establishment of Kit for superoxide dismutase activity. Anal. Biochem., 42: 290-296
  13. Gao, R., Yuan, Z., Zhao, Z., and Gao, X. (1998) Mechanism of pyrogallol autoxidation and determination of superoxide dismutase enzyme activity, Bioelectrochem. Bioener., 45: 41-45 https://doi.org/10.1016/S0302-4598(98)00072-5
  14. Aebi, H. (1974) Catalase in the Method of enzymatic analysis-( H. U. vergmeter, ed.). Academic press, New York, 2: 673-675
  15. Berenice, C. W., Michel, V, Allain, F, Catherine, G, Veronique, D., Josianne A., and Marie, J. R. (1992) Selenium, glutathione peroxidase (GSH-Px) and lipid peroxidation products before and after selenium supplementation. Clin.Chim. Acta, 207: 137-142 https://doi.org/10.1016/0009-8981(92)90157-L
  16. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Rendall (1951) Protein Measurement with folin phenol reagent. J. Biol. Chem., 256: 9994-9998
  17. Raul, G. M., Ivete, A. R., and Mario, H. H. (1990) Effects of triton WR-1339 and heparin on the transfer of surface lipids from triglyceride rich emulsions to HDL in rats, Lipid, 25: 7012-705
  18. Goldstein, J. L. and Brown, M. S. (1975) Familial hypercholesterolemia. A genetic regulatory defect in cholesterol metabolism, Am. J. Med., 58: 147-150 https://doi.org/10.1016/0002-9343(75)90563-X
  19. Minor, L. K., Mahlberg, E H., Jerome, W. G, Leis, J. C., Rothblat, G H., and Glick, J. M. (1991) Lysosomal hydrolysisof lipids in a cell culture model of smouth muscle foam cells, Experimental and Molecular Pathology, 54: 159-171 https://doi.org/10.1016/0014-4800(91)90028-V
  20. Gilland, F. D., Berhane, K. T., Li, Y. F., Gauderman, W. J., McConnell, R., and Peters, J. (2003) Children's lung function abd antioxidant vitamin, fruit, juice, and vegetable intake, Amenrican Journal of Epidemiology, 158: 576-584 https://doi.org/10.1093/aje/kwg181
  21. Urano, S., Asai, Y., Matake, S., Matsuo, S., Izumiyama, M., Ohtsubo, K., and Endo, T. (1997) Oxidative injury of synapse and alteration of antioxidative defence systems in rats, and its prevention by vitamin E, European Journal of Biochemistry, 245: 64-70 https://doi.org/10.1111/j.1432-1033.1997.00064.x
  22. Rodriguez-Porcel, M., Lerman, L. O., Holmes, D. R., Richardson, D., Napoli, C., and Lerman, A. (2002) Chronic antioxidant supplementation attenuates nuclear factor-B activation nd preserves endothelial function in hypercholesterolemic pigs, Cardiovascular Research, 53: 1010-1018 https://doi.org/10.1016/S0008-6363(01)00535-1
  23. Fang, Y. Z., Yang, S., and Wu, G (2002) Free radicals, antioxidants, and nutrition, Nutrition, 18: 872-879 https://doi.org/10.1016/S0899-9007(02)00916-4