Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Screening of Thrombin Inhibitor from Medicinal and Wild Plants (II)

약용 및 야생식물로부터 트롬빈 저해물질의 탐색 (II)

  • Ryu, Hee-Young (Department of Food and Nutrition, Andong National University) ;
  • Kwon, Yun-Sook (Department of Food and Nutrition, Andong National University) ;
  • Kum, Eun-Joo (Department of Food and Nutrition, Andong National University) ;
  • Kwon, Chong-Suk (Department of Food and Nutrition, Andong National University) ;
  • Kwon, Gi-Seok (The School of Bioresource Sciences, Andong National University) ;
  • Kim, Kun-Woo (The School of Bioresource Sciences, Andong National Universuty) ;
  • Son, Kun-Ho (Department of Food and Nutrition, Andong National University) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
  • 류희영 (안동대학교 식품영양학과) ;
  • 권윤숙 (안동대학교 식품영양학과) ;
  • 금은주 (안동대학교 식품영양학과) ;
  • 권정숙 (안동대학교 식품영양학과) ;
  • 권기석 (안동대학교 생명자원과학부) ;
  • 김건우 (안동대학교 생명자원과학부) ;
  • 손건호 (안동대학교 식품영양학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Published : 2005.12.30
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Abstract

To develop safe and new anticoagulation agents from natural resources, the inhibitory activities of 291 methanol extracts, which were prepared from different parts of 197 medicinal and wild plants, against human thrombin were evaluated. Based in anti-coagulation activity determined by thrombin time and activated partial thromboplastin time, the extract of leaf of Myrica rubra was finally selected. The extract of M. rubra showed a strong thrombin inhibitory activity (above 1,819%) at 0.5 mg/ml as a final concentration, whereas aspirin showed 337% inhibition at concentration of 1.5 mg/ml. The activity of the extract remained more than 85% and 60% by heat treatment at $100^{\circ}C$ for 30min, and acid treatment at pH 2 for 60 min, respectively. Our results suggested that the extract of Myrica rubra could be the potential source as thrombin inhibitor.

Keywords

References

  1. Butenas, S. and Mullertz, S. (2002) Blood coagulation. Biochemistry (Moscow) 67: 3-12 https://doi.org/10.1023/A:1013985911759
  2. Korea National Statistical Office. (2000) A study on causes of death for 1999
  3. Cattaneo, F., Trento, F., Pescador, R., Porta, R. and Furro, L. (2002) Pharmacodynamics of the anticoagulant activity (APTT) of an algal polysaccharide. Thromb. Res. 105: 455-457 https://doi.org/10.1016/S0049-3848(02)00047-6
  4. Molina, V, Arruzazabala, M. L. and Mas, C. R. (2003) Synergistic effect of D-003 and aspirin on experimental throm-bosis models. Prostag.Leukotr. Ess. 68: 305-310 https://doi.org/10.1016/S0952-3278(03)00020-6
  5. Katira, R., Chauhan, A. and More, R. S. (2005) Direct thrombin inhibitors: novel antithrombotics on the horizon in the thromboprophylactic management of atrial fibrillation. Postgrad. Med. J. 81: 370-375 https://doi.org/10.1136/pgmj.2004.024521
  6. Weitz, J. I. and Crowther, M. (2002) Direct thrombin inhibitors. Thromb. Res. 106: 275-284 https://doi.org/10.1016/S0049-3848(02)00093-2
  7. Gwak, H.-S. and Chun, I. K. (2000) Performulation study of aspalatone, a new antithrombotic agent. J. Appl. Pharmacol. 8: 332-337
  8. Hsieh, K.-H. (1997) Thrombin interaction with fibrin polymerization sites. Thromb. Res. 86: 301-316 https://doi.org/10.1016/S0049-3848(97)00073-X
  9. Hellgren, M., Johansson, S., Eriksson, U. G and Wahlander, K. (2005) The oral direct thrombin inhibitor, ximelagatran, an alternative for anticoagulant treatment during the puerperium and lactation. BJOG 112: 579-583 https://doi.org/10.1111/j.1471-0528.2004.00494.x
  10. Gulseth M. P. (2005) Ximelagatran: an orally active direct thrombin inhibitor. Am. J. Health. Syst. Pharm. 62: 1451-1467 https://doi.org/10.2146/ajhp040534
  11. Lee, T.-B. (1993) Illustrated flora of Korea. Hwang Moon Sa, Seoul
  12. Sooo, H.-Y, Kwon, Y-S., Kim, Y-S., Kwon, H.-Y, Kwon, G-S., Kim, G-J., Kwon, C.-S. and Son, K. H. (2004) Screening of thrombin inhibitors from medicinal and wild plants. Kor. J. Pharmacogn. 35: 52-61
  13. Kwon, C.-S., Kwon, Y-S., Kim, Y-S., Kwon, G-S., Jin, I., Ryu, G-C. and Sooo, H.-Y (2004) Inhibitory activities of edible and medicinal herbs against human thrombin. J Life Sci. 14: 509-513 https://doi.org/10.5352/JLS.2004.14.3.509
  14. Lee, H.-J., Kim, J.-S., Heo, G-Y, Lee, K,-B., Rhee, I.-K. and Song, K.-S. (1999) Inhibitory activities ofbasidiomycetes on prolyl endopeptidase, acetylcholine esterase and coagulation. J. Korean Soc. Agri. Chem. Biotechnol. 42: 336-343
  15. Hahn,B.-S., Wu, S. and Kim, Y-S. (1999) Evaluation of anticoaguant and fibrinolytic activities from crude extracts. Kor. J. Pharmacogn. 30: 409-412
  16. Soho, H.-Y, Kwon, C.-S., Son, K.-H., Kwon, G-S., Kwon, Y-S., Ryu, H.-Y. and KUlIl, E.-J. (2005) Antithrombosis and antioxidant activity ofmethanol extract from different brands of rice. J. Kor. Soc. Food Sci. Nutr. 34: 593-598 https://doi.org/10.3746/jkfn.2005.34.5.593
  17. Bao, J., Cai, Y, Sun, M., Wang, G and Corke, H. (2005) Anthocyanins, flavonols, and free radical scavenging activity of Chinese bayberry (Myrica rubra) extracts and their color properties and stability. J. Agric. Food Chem. 53: 2327-2332 https://doi.org/10.1021/jf048312z
  18. Kuo, P. L., Hsu, Y. L., Lin, T. C., Lin, L. T. and Lin, C. C. (2004) Induction of apoptosis in human breast adenocarcinoma MCF-7 cells by prodelphinidin B-2 3, 3'-di-O-gallate from Myrica rubra via Fas -mediated pathway. J. Pharm. Pharmacol. 56: 1399-1406 https://doi.org/10.1211/0022357044625