Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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In vitro Antioxidant Activity of Mentha viridis L. and Mentha piperita L.

박하의 in vitro 항산화활성

  • Published : 2005.12.30
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Abstract

For selecting a new candidate as functional material, this study was conducted on in vitro antioxidant activity and total phenol content of methanol and water extracts prepared from two Mentha species (M viridis L. (M spicata L.) and Mentha piperita L, Extracts of M. viridis showed more efficient scavenging activity on superoxide and DPPH ${({\alpha},{\alpha}-diphenyl-{\beta}-picrylhydrazyl)}$ radical and inhibitory activity on oxidation of human low density lipoprotein (LDL) induced by $CuSO_4$ and auto-oxidation of linoleic acid than those of M piprita. Methanol extract $(65.88%{\sim}77.59%)$ and water extract $(37.69%{\sim}87.21%)$ of M. viridis also exhibited more potent inhibitory activity on LDL oxidation than that of ${{\alpha}-tocopherol\;(28.37%{\sim}66.54%)}$ at ${1{\sim}100\;{\mu}g/ml}$ of final concentration. The total phenol contents of methanol extract and water extract of Mviridis (17.95% & 10.18%, respectively) as tannic acid equivalent were higher than those of M piperita (15.44% & 9.19%). But the yields of methanol and water extracts of M. viridis (13.3 % & 13.5%) were lower than those of M. piperita (14.1 % & 14.6%). The results implies that the extracts from M. viridis (spicata) is more useful material for industrialization as functional food than those from M. piperita.

새로운 기능성 소재를 발굴하기 위한 목적으로 2종의 박하(M. viridis (spicata), M. piperita)를 대상으로 수행된 몇 가지 in vitro 항산화계에서의 활성, 총페놀 함량 및 수율을 분석한 결과는 다음과 같다. 1. Superoxide 라디칼에 대한 박하의 소거능은 M. viridis가 M. piperita 보다 우수하였으며 특히 물 추출물은 대조물질인 ascorbic acid보다 우수하였다. 2. DPPH 라디칼에 대한 소거능은 메탄올 추출물 및 물 추출물 모두에서 M. viridis가 M. piperita보다 우수하였으며 특히 M. viridis의 메탄올 추출물은 롤${\alpha}-tocopherol$과 비슷한 수준이었다. 3. 사람의 저밀도지단백질의 산화에 대한 박하의 저해활성을 분석한 결과는 M. viridis가 M.piperita 보다 우수하였으며 M. viridis의 모든 추출물 ${(65.88%{\sim}77.59%,\;37.69%{\sim}87.21%)}$은 ${{\alpha}-tocopherol\;(28.37%{\sim}66.54%)}$보다 월등하게 우수하였다. 4. Linoleic acid의 산화에 대한 저해효과는 M. viridis가 M. piperita보다 높았으며 ${\alpha}-tocopherol$의 활성에 근접하는 수준이었다. 5. 총페놀 함량은 M. viridis (17.95% & 10.18%)가 M. piperita (15.44% & 9.19%)보다 높은 값을 나타내었으며 메탄올 추출물이 물 추출물보다 그 함량이 높았다. 6. 추출물의 수율은 메탄을 추출물 및 물 추출물 모두에서 M. piperita (14.1% & 14.6%)가 M. viridis (13.3% & 13.5%)보다 높게 나타나 항산화활성 및 총페놀 함량과는 상반되는 결과를 나타내었다. 이상의 실험결과를 종합할 때, 실험된 박하 중에서 M. viridis가 M. piperita보다 우수한 산업화 후보 자원이라고 사료된다.

Keywords

References

  1. Medicinal plants of Korea Bae, K.H.
  2. Phytotherapy research v.14 Screening for antimycotic properties of 56 traditional chinese drugs Blaszczyk, T.;Krzyzanowska, J.;Lammer-Zarawska, E.
  3. Nature v.181 Antioxidant determinations by the use of a stable free radical Bloi, M.S.
  4. Korean J. Food Sci. Technol. v.14 Toxicological studies of antioxidants, butylated hydroxy toluene (BHT), and butylated hydroxy anisole (BHA) Choe, S.Y.;Yang, K.H.
  5. Antioxidant properties and composition of acqueous extracts from Mentha species, hybrids, varieties and cultivars Dorman, H.J.D.;Kosar, M.;Kahlos, K.
  6. J. Nutritional Biochemistry v.13 Effects of an antioxidant-rich juice (sea buckthorn) on risk factors for coronary heart disease in humans Eccleston, C.;Baoru, Y.;Tahvonen, R.;Kallio, H.;Rimbach, G.H.;Minihane, A.M.
  7. Pahrmacological Research v.52 Protective effect of red seeds proanthocyanidins against induction of diabetes by alloxan in rats El-Alfy, A.T.;Ahmed, A.A.E.;Fatani, A.A.J.
  8. Free radicals and oxidative stress : environment, drugs and food additives Evance, C.R.;Halliwell, B.;Lunt, G.G.
  9. Trends Cardiovac. Med. v.11 Mechanisms for oxidizing low-density lipoprotein Gaut, J.P.;Heinecke, J.W.
  10. Life Science v.71 Three different pathways for human LDL oxidation are inhibited in vitro by water extracts of the medicinal herb Gugliucci, A.;Menini, T.
  11. J. Agric. Food Chem. v.40 Antioxidative substances in leaves of Polygonum hydropiper Haraguchi, H.;Hashimoto, K.;Yagi, A.
  12. Biol. Pharm. Bull. v.24 Effects of peppermint (Mentha piperita L.) extracts on experimental allergic rhinitis in rats Inoue, T.;Sugimoto, Y.;Masuda, H.;Kamei, C.
  13. Korean J. Food Sci. Technol. v.25 Effect of solvents and some extraction conditions on antioxidant activity in cinnamon extracts Kim, N.M.;Sung, H.S.;Kim, W.J.
  14. J. Agric. Food Chem. v.52 Screening of free radical scavenging compounds in water extracts of mentha samples using a postcolumn derivatization method Kosar, M.;Dorman, H.J.D.;Basar, K.H.C.;Hiltunen, R.
  15. Biofactors v.22 Anticancer and radioprotective potentials of Mentha piperita Kumar, A.;Samarth, R.M.;Yasmeen, S.;Sharma, A.;Sugahara, T.;Terado, T.;Kimura, H.
  16. CRC critical Rev. Food Technol. Kinetics of lipid oxidation in foods Labuza, T.P.
  17. Steroids v.61 In vitro antioxidant effects of estrogens with a hindered 3-OH function on the copper-induced oxidation of low density lipoprotein Miller, C.P.;Jirkovsky, I.;Hayhurst, D.A.;Adelman, S.J.
  18. Biochem. Biophys. Res. Commun. v.46 The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen Nishikimi, M.;Rao, N.A.;Yagi, K.
  19. Indian J. Exp. Biol. v.41 Mentha (Linn.) leaf extract provides protection against radication induced chromosomal damage in bone marrow of mice Samarth, R.M.;Kumar, A.
  20. Eur. J. Clin. Chem. Clin. Biochem. v.32 Ethanol-induced oxidative stress and membrane injury in rat erythrocytes Sozmen, E.Y.;Tanyakin, T.;Onat, T.;Kufay, F.;Erlacin, S.
  21. International Journal of Toxicology v.3 Final report on the safety assessment of Mentha piperita (peppermint) oil, Mentha piperita (peppermint) leaf extract, Mentha piperita (peppermint) leaf, and Mentha piperita (peppermint) leaf water Taylor, F.
  22. Biol. Pharm. Bull. v.21 Anti-HIV-1 activity of herbs in Labiatae Yamasaki, K.;Nakano, M.;Kawahata, T.;Mori, H.;Otake, T.;Ueda, N.;Oishi, I.;Inami, R.;Yamane, M.;Nakamura, M.;Murata, H.;Nakanishi, T.
  23. Environ. Mol. Mutagen. v.44 Antimutagenic activity of spearmint Yu, T.W.;Xu, M.;Dashwood, R.H.
  24. J. Agric. Food Chem. v.49 Antioxidant activity and phenolic compounds in selected herbs Zheng, W.;Wang, S.Y.