Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Skin Permeation and Crosslinking with Biological Tissue of the Hydrolyzed Products of Gardeniae Fructus Extract

치자엑스 가수분해물 제제의 피부투과 및 생체 피부조직과의 교차결합에 관한 연구

  • Published : 2005.02.20
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Abstract

Gardeniae Fructus is consisted of geniposide and it's derivatives. For the purpose of treatment of skin disease, geniposide and hydrolyzed products (HP) of Gardeniae Fructus were studied on skin permeation and cross1inking with biological tissue. The hydrolyzed products (HP) and active ingredients of Gardeniae Fructus were identified and investigated about skin permeability. Genipin has provided low cytotoxic cross1inking reagents and formed stable and biocompatible crosslinked products. The permeation enhancing effects of geniposide and genipin under the hydrolyzed products of cream and hydrogel preparations were tested using Franz type diffusion cell and the skin of hairless mouse. The remaining proportions of geniposide and genipin were measured in the hydrolyzed products of cream and hydrogel preparations. The crosslinking of epidermic and endodermic tissue with genipin under the hydrolyzed prodcuts of cream and hydrogel preparation was observed using light microscopy. Increased absorption ratio of the skin of hairless mouse about genipin was higher than that of geniposide. Loads at break, tensile strengths and skin permeation rate of the hydrolyzed products (HP) of cream and hydrogel preparations were higher than the nonhydrolyzed products (NHP). The hydrolyzed products (HP) of cream and hydrogel of Gardeniae Fructus Extracts were proper preparations and crosslinking agents to increase the transdermal absorption with epidermic and endodermic tissue.

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References

  1. Y. Kawata, M. Hattori, T. Akao, K. Kobashi and T. Namba, Formation of nitrogen containing metabolites from geniposide and gardenoside by human intestinal bacteria, Planta Med., 57, 536-542 (1991) https://doi.org/10.1055/s-2006-960201
  2. D.U. Lee, C.H. Park, S.I. Kang, E.G. Min, Y.H. Han and C.K. Lee, Isolation of the component transformed into blue pigments by aerobic bateria in the fruits of gardenia jasminoides, Kor. J. Pharmacogn., 29(3), 204-208 (1998)
  3. Y. Paik, C. Lee, M. Cho and T. Hahn, Physical stability of the blue pigments formed from geniposide of gardenia fruits: effects of pH, temperature and light, J. Agric. Food Chem., 49(1), 430-432 (2001) https://doi.org/10.1021/jf000978f
  4. Y.N. Han, H.K. Oh, K.H. Hwang and M.S. Lee, Antioxidant components of gardenia Fruit, Kor. J. Pharmacogn., 25(3), 226-232 (1994)
  5. Y. Liu, G.R. Lu and J. Lu, HPLC determination of iridoids in cape jasmine fruit(zhizi), Zhingguo Zhong Yao Za Zhi, 18(5), 298-299 (1993)
  6. T. Oshima, K. Sagara, T. Yoshida, Y.Y. Tong, G.D. Zhang and Y.H. Chen, Determination of geniposide, gardenoside, geniposidic acid and genipin-1-beta-gentiobioside in gardenia jasminoides by high performance liquid chromatography, J. Chromatogr., 25(455), 410-414 (1988)
  7. T. Akao, K. Kobashi and M. Aburada, Enzymic studies on animal and intestinal bacterial metabolismof geniposide, Bio. Pharm. Bull., 17(12), 1573-1576 (1994) https://doi.org/10.1248/bpb.17.1573
  8. F.L. Mi, Y.C. Tan, H.C. Liang, R.N. Huang and H.W. Sung, In vitro evaluation of a chitosan membrane cross-linked with genipin, J. Biomater Sci Polym Ed., 12(8), 835-850 (2001) https://doi.org/10.1163/156856201753113051
  9. Y. Chang, C.C. Tsai, H.C. Liang and H.W. Sung, In vivo evaluation of celluar and acellular bovine pericadia fixed with a naturally occurring crosslinking agent(genipin), J. Biomater Sci, 12(8), 851-852 (2001) https://doi.org/10.1163/156856201753113060
  10. C.C. Tsai, Y. Chang, H.W. Sung, J.C. Hsu and C.N. Chen, Effects of heparin immobilization on the surface characteristics a biological tissue fixed with a naturally occurring crosslinking agent (genipin): an in vitro study, Biomaterials, 22(6), 523-533 (2001) https://doi.org/10.1016/S0142-9612(00)00206-4
  11. Lynn L.H. Huang, Hsing-wen Sung, Chen-Chi Tsai, DonMou Huang, biocompatibility study of a biological tissue fixed with a naturally occurring crosslinking reagent, J. Biomed. Mater. Res. 42(4), 568-576 (1998) https://doi.org/10.1002/(SICI)1097-4636(19981215)42:4<568::AID-JBM13>3.0.CO;2-7
  12. Hsing-wen Sung, Rong-Nan Huang, Lynn L. H. Huang, Chen-Chi Tsai, Chi-Tung Chiu, feasibility study of a natural crosslinking reagent for biological tissue fixation, Journal of Biomedical Materials Research, 42(4), 560-567 (1998) https://doi.org/10.1002/(SICI)1097-4636(19981215)42:4<560::AID-JBM12>3.0.CO;2-I
  13. H.W. Sung, C.N. Chen, R.N. Huang, J.C. Hsu, W.H. Chang, In vitro surface characterization of a biological patch fixed with naturally occurring crosslinking agent, Biomaterials, 21(13), 1353-1362 (2000) https://doi.org/10.1016/S0142-9612(00)00017-X
  14. J.H. Yang and N.H. Lee, Skin Permeation and Antiinflammatory Effects of Hydrolyzed Products of Gardeniae Fructus Extracts, Journal of Korean Pharmaceutical Sciences, 34(2), 115-123 (2004)
  15. S.H. Suh, M.T. Cho, B.B. Lee and D.I. Kim, Development of the unsteady capillary tube viscometer and viscosity measurement of blood analogue fluid, Journal of the Korean Society for Vascular Surgery, 18(1), 23-30 (2002)
  16. W.S. Ha, I.J. Roh and J.H. Kim, Surface tension and antithrombogenicity of silk fibroin films graft-copolymerized with methyl methacrylate and styrene by cold methane plasma initiation, Journal of the Korean Society of Textile Engineers and Chemists, 26(4), 33-39 (1989)
  17. B.H. Park and Y.C. Ko, Temperature effect on tensile strength of filled natural rubber vulcanizates, Elastomer, 36(4), 255-261 (2001)
  18. K. Sato, K. Sugibayashi and Y. Morimoto, Effect and mode of action of aliphatic esters on the in vitro skin permeation of nicorandil, Int. J. Pharm., 43, 31-40 (1988) https://doi.org/10.1016/0378-5173(88)90055-5