Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
권호 표지

Effect of Feeding Chitosan, Hibiscus Extract and L-Carnitine Mixture on Body Weight and Lipid Metabolism in Rats

키토산, 히비스커스 추출물 및 L-카르니틴 함유 식이가 흰쥐의 체중과 지질대사에 미치는 영향

  • Published : 2005.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was peformed to investigate effect of feeding experimental mixture containing chitosan, hibiscus extract and L-carnitine on body weight and lipid metabolism in rats. Forty-eight male rats(Charles River CD) of eight weeks old and weighing $336.5{\pm}2.3g$ were raised for five weeks with high fat diet(40% fat as calorie) to induce obesity. After induction of obesity, rats weighing $560.4{\pm}5.6g$ were blocked into four groups according to body weight and raised for eight weeks with diet containing either 0.09%(+1D group), 0.9%(+10D group) or 4.5%(+50D group) of experimental mixture. Aspartate aminotransferase(AST) and alanine aminotransferase(ALT), total protein and albumin were normal levels in plasma. Body weight gain and epididymal fat pad weight were lower in experimental mixture groups than control group However, weights of perirenal fat pad and brown adipose tissue were not significantly different among all groups. There was no significant difference in plasma and hepatic lipid levels among all groups. Liver citrate lyase and camitine acyltransferase activities were not significantly different among all groups, however, citrate lyase activity was tended to be decreased with increasing experimental mixture level in diet. Fecal total lipid and total cholesterol excretions were highest in +50D group, and triglyceride excretion was highest in +1D group. in conclusion, intake of experimental mixture containing chitosan, hibiscus extract and L-camitine was effective in reducing body weight and body fat, and its inhibitory effects might lead to obesity improvement.

Keywords

References

  1. 2001년 국민건강.영양조사 총괄편 보건복지부
  2. Korean J Nutr v.31 no.2 Effects of chitosan of different molecular weights on lipid metabolism in rats Lee, Jong-Mee;Son, Bo-Kyung
  3. Korean J Nutr v.30 no.7 Effect of chitosan and N,O-carboxymethyl chitosan of different sources and molecular weights on cadmium toxicity Bea, Kye-Hyun;Kim, Mi-Kyung
  4. J Nutr v.130 Chronic(-) hydroxycitrate administration spares carbohydrate utilization and promotes lipid oxidation during exercise in mice Ishihara, K.;Oyaizu, S.;Onuki, K.;Lim, K.;Fushiki, T.
  5. Nutrition v.17 Necessity of carnitine supplementation in semistarved rats fed a high-fat diet Feng, Y.;Guo, G.;Wei, J.;Yang, J.;Ge, Y.;Gao, L. https://doi.org/10.1016/S0899-9007(01)00601-3
  6. J Vet Intern Med v.14 no.6 The clinical and metabolic effects of rapid weight loss in obese pet cats and the influence of supplemental oral L-carnitine Center, S.A.;Harte, J.;Watrous, D.;Reynolds, A.;Watson, T.D.;Markwell, P.J.;Millington, D.S.;Wood, P.A.;Yeager, A.E.;Erb, H.N. https://doi.org/10.1892/0891-6640(2000)014<0598:TCAMEO>2.3.CO;2
  7. 만성질환 실태와 관리방안 오진주
  8. Acta Toxicol Ther v.17 no.1 Dose effect in lipidlowering activity of a new dieatary integrator (Chitosan, Garcinia cambogia extract and chrome) Girola, M.;Bernardi, M.;Cantos, S.;Tripodi, S.;Ventura, P.;Guarino, C.;Marletta, M.
  9. J Nutr v.123 AIN-93 purified diets for laboratory rodents: Final report of the American institute of nutrition Ad Hoc writing committee on the reformulation of the AIN-76A rodent diet Reeves, P.G.;Nielsen, F.H.;Fahey, G.C.
  10. Am J Clin Nutr v.53 A colorimetric method for determination of total serum lipid based on the sulfuric-phospho-vanillin reaction Frings, C.S.;Dunn, R.T.
  11. Can J Bwchem Physiol v.37 A rapid method of total lipid extraction and purification Bligh, E.G.;Dyer, W.J. https://doi.org/10.1139/o59-099
  12. Micro-kjeldal method. microchemical determination of nitrogen, AOAC official method 960.52
  13. J Clin Invest v.98 no.10 Adenosine triphosphate citrate lyase mediates hypocitratunia in rats Melnick, Joel Z.;Srere, Paul A.;Elshourbagy, Nabil A.;Moe, Orson W.;Preisig, Patricia A.;Alpern, Rpbert J. https://doi.org/10.1172/JCI119051
  14. Kidney Int v.57 Renal citrate metabolism and urinary citrate excretion in the infant rat Melnick, Joel Z.;Preisig, Patricia A.;Alpern, Rpbert J.;Baum, Michel https://doi.org/10.1046/j.1523-1755.2000.057003891.x
  15. J Biol Chem v.34 no.10 The citrate cleavage enzyme Srere, Paul A.
  16. Arch Biochem Biophys v.130 The sulfhydryl groups of citrate cleavage enzyme Cottam, Gene L.;Srere, Paul A. https://doi.org/10.1016/0003-9861(69)90037-X
  17. Methods Enzymol ATP citrate lyase Takeda, Y.;Suzuki, F.;Inoue, H.
  18. Nutrition v.17 Effects of conjugated linoleic acid on serum leptin concentration, body-fat accumulation, and ${\beta}$-oxidation of fatty acid in oletf rats Rahman, Shaikh Mizanoor;Wang, Yu-Ming;Yostumoto, Hiroaki;Cha, Jae-Young;Han, Seo-Young;Inoue, Shuji;Yanagita, Teruyoshi https://doi.org/10.1016/S0899-9007(00)00584-0
  19. Biosci Biotechnol Biochem v.62 no.4 Effects of dietary a-linolenic, eicosapentaenoic and docosahexaenoic acids on hepatic lipogenesis and j3-oxidation in rats Ikeda, Ikuo;Cha, Jae-Young;Yanagita, Teruyoshi;Nakatani, Noriaki;Oogami, Kazuhiro;Imaizumi, Kastumi;Yazawa, Kazunaga https://doi.org/10.1271/bbb.62.675
  20. J Biol Chem v.218 no.10 The subcellular distribution of carnitine acyltransferases in mammalian liver and kidney Markwell, Mary Ann K.;Mcgroarty, Estele J.;Bieber, Loran L.;Tolbert, N.E.
  21. Arch Med Res v.32 Oral theophylline changes renal carnitine palmitoyltransferase activity in rats Alhomida, Abdullah S. https://doi.org/10.1016/S0188-4409(01)00299-5
  22. J Biol Chem v.193 Protein measurement with folin phenol reagent Lowry, O.H.;Rosebrough, N.J.;Farr, A.L.;Randall, R.J.
  23. Korean J Lab Ami Sci v.10 no.1 The effect of fasting time on haematological and blood biochemical parameters in SO rats Song, Chang-Woo;Cho, Kyu-Hyeok;Han, Hyun-Sook;Han, Sang-Seop
  24. Baseline data comparing CO(SO)IGS rats supplied from Charles River Japan Hasegawa, K.;Larson, J.L.;White, W.J.;Clifford, C.B.
  25. 식품영양실험핸드북. 영양편 한국식품영양과학회
  26. Korean J Nutr v.34 no.5 Effect of dry powders, ethanol extracts and juices of radish and onion on lipid metabolism and antioxidative capacity in rats An, So-Jin;Kim, Mi-Kyung
  27. Korean J Nutr v.35 no.7 Effect of grape intakes on lipid metabolism of rats during aging Um, Min-Young;Kim, Mi-Kyung