Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
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Anticariogenic Effects and Inhibition of Glucosyltransferase Activity of Chrysanthemun indicum L. Extracts

감국(Chrysanthemun indicum L.) 추출물의 항충치효과와 Glucosyltransferase 저해활성 탐색

  • Kim, Seon-Jae (Dept. of Food Engineering and Food Industrial Technology Research Center, Mokpo National University(RRC)) ;
  • Park, Yun-Mi (Dept. of Food Engineering and Food Industrial Technology Research Center, Mokpo National University(RRC)) ;
  • Jung, Soon-Teck (Dept. of Food Engineering and Food Industrial Technology Research Center, Mokpo National University(RRC))
  • 김선재 (목포대학교 식품공학과 및 식품산업기술연구센터) ;
  • 박윤미 (목포대학교 식품공학과 및 식품산업기술연구센터) ;
  • 정순택 (목포대학교 식품공학과 및 식품산업기술연구센터)
  • Published : 2005.06.30
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Abstract

The anticariogenic effects and inhibition of glucosyltransferase activity of Chrysanthemun indicum L. extracts were investigated against 5 strains of microorganisms. The ethanol extracts of Chrysanthemun indicum L. showed the growth inhibition effects on the Streptococcus mutans and oral bacteria. The inhibition rate of glucosyltransferase activity of Chrysanthemun indicum L. extracts showed 78.4 to 92.3% range. The extracts were analysed by using solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 column chromatography, TLC, HPLC techniques. Three components whose molecular weights ranged from 200 to 400 were confirmed to have the anticariogenic activity.

감국에 존재하는 항충치활성 물질 및 glucosyltransferase 저해 효과에 대한 정보를 얻고자 본 연구를 시도하였다. 감국 ethanol 추출물의 항충치활성을 S. mutans 및 구강세균에 대해 검색한 결과, S. mutans와 2종의 구강세균에 대하여 활성이 높게 나타났다. 감국 ethanol 추출물의 농도에 따라 GTase 저해효과는 $78.4{\sim}92.3%$의 범위로 나타나 농도가 증가할 수록 활성도 함께 증가하는 경향을 나타냈다. 감국을 ethanol로 추출, 용매분획하여 얻어진 활성성분을 silica gel adsorption chromatography, Sephadex LH-20 chromatography, TLC 그리고 HPLC 등의 방법으로 분석한 결과, 감국에 존재하는 항충치활성 물질은 약간의 극성을 띠는 중성물질로 분자량이 200-400정도 되는 저분자 물질인 것으로 확인되었다.

Keywords

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