Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antimicrobial Effects of Camellia Japonica L. Leaves Extract on Food-borne Pathogenic Microorganisms

동백나무(Camellia japonica L.) 잎 추출물이 식품유해 미생물에 미치는 항균 효과

  • 한영숙 (성신여자대학교 식품영양학과)
  • Published : 2005.02.28
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Abstract

Antimicrobial effects of Camellia japonica L. were determined against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Listeria monocytogenes using paper disc method, and minimum inhibitory concentrations (MICs) were measured. Methanol extract (MEex), water fraction (WAfr), and butanol fraction(BUfr) showed antimicrobial effects against all tested microorganisms, with MEex showing strong antimicrobial effect against S. aureus and L. monocytogenes, and WAfr, Bufr, and ethylacetate fraction (EAfr)against S. aureus. No effects were observed in n-hexane fraction (HEfr) and chloroform fraction (CHfr) against all tested microorganisms. All species grown in the medium adding fractions of Camellia Japonica L. leaves extact were inhibited from WAfr and BUfr, repectively.(meaning not clear) MEex showed over 25% inhibitory effect against all tested microorganisms. BUfr showed over 50% inhibitory effect against all microorganisms except L. monocytogenes. EAfr and WAfr showed over 30% effect against S. aureus and L. monocytogenes. MICs of MEex against S. typhimurium and BUfr against S. aureus were 625 g/mL, indicating C. japonica L. extract can exert antimicrobial activity even at low concentration.

인체에 무해한 천연 항균 소재를 탐색하기 위한 목적으로 국내에 자생하는 동백나무(Camellia japonica L.) 잎의 methanol 추출물 및 분획물로 4종의 식품 유해 미생물(Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Listeria monocytogenes)에 대한 항균활성을 살펴보았다. Paper disc 법을 사용하여 조사한 용매별 항균효과는 methanol 추출물이 4종의 시험균주 모두에서 항균효과를 나타내었다. 특히 S. aureus와 L. monocytogenes에 대하여 강한 항균효과를 나타내었다. 분획물 중에서는 water 분획물과 butanol 분획물이 S. aureus에 대하여 강한 항균효과를 나타내었다. n-hexane 분획물과 chloroform 분획물은 4종의 시험균주 모두에서 항균효과를 나타내지 않았으며, ethylacetate 분획물은 S. aureus에 대해서만 항균효과를 나타내었다. 동백나무 잎 methanol 추출물과 용매별 분획물을 농도별로 처리하여 균들의 생육도를 균주별로 조사한 결과, 공통 적으로 모든 균들이 methanol 추출물에서 항균효과를 나타내었고, 분획물 중에서는 butanol 분획물, water 분획물에서 항균효과가 뚜렷이 나타났다. 항균 저해율을 확인한 결과, methanol 추출물은 모든 균에 대해 약 25% 이상의 저해율을 나타내었다. 분획물 중에서는 butanol 분획물이 L. monocytogenes를 제외한 균에서 50% 이상의 강한 저해율을 나타내었으며 ethylacetate 분획물과 water 분획물에서는 각각 S. aureus, L. monocytogenes가 30% 이상의 높은 생육 저해율을 나타내었다. 균의 최소저해 농도(MIC)를 측정한 결과는 S. typhimurium에 대하여 methanol 추출물이 $625{\mu}g/mL$의 농도를 나타내었다. 분획물 중에서 S. aureus에 대하여 butanol 분획물이 $625{\mu}g/mL$의 농도로 가장 낮은 값을 나타내어 적은 양으로도 항균활성을 나타낼 수 있음을 알 수 있었다.

Keywords

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