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Root Rot of Japanese Angelica Caused by Phytophthora cactorum in Nursery and Mycological Characteristics of the Isolates

두릅나무 묘목생산포의 역병 발생 및 분리균의 균학적 특성

  • Lee, Sang-Hyun (Department of Forest Diseases and Insect Pests, Korea Forest Research Institute) ;
  • Lee, Jae-Pil (Department of Forest Diseases and Insect Pests, Korea Forest Research Institute) ;
  • Kim, Kyung-Hee (Department of Forest Diseases and Insect Pests, Korea Forest Research Institute) ;
  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, College of Life and Environmental Sciences, Korea University)
  • 이상현 (국립산림과학원 산림병해충과) ;
  • 이재필 (국립산림과학원 산림병해충과) ;
  • 김경희 (국립산림과학원 산림병해충과) ;
  • 신현동 (고려대학교 환경생태공학부)
  • Published : 2005.12.30

Abstract

In 2003 to 2005, the root rot of Japanese angelica (Aralia elata) was surveyed in nursery beds of Korea, where incidence of the disease often reached up to 100%. Three isolates were obtained from the infected roots, and identified as Phytophthora cactorum on the basis of cultural, morphological characteristics and molecular analysis. The isolates were characterized by having markedly papillate and broadly ovoid deciduous sporangia. The optimum temperature for mycelium growth was at $25^{\circ}C$ on V8 juice agar. Pathogenicity of the isolates was confirmed by soil mixture inoculation. Approximately 900 bp of ITS rDNA was amplified from all 3 isolates and band pattern of restriction fragments observed by Alu I, Msp I, and Taq I digestion also supported the result of the morphological identification when compared with PhytID database.

2003년부터 2005년 사이에 한국의 두릅나무에 심각한 역병이 발생하였다. 감염된 두릅나무와 토양으로부터 병원균이 분리되었으며, 이 균은 배양적인 그리고 형태적인 특징 및 병원성 검증 실험에 의해 P. cactorum(Lebert&Cohn) J. Schrt.으로 동정되었다. 이 균은 뚜렷한 돌출형이고, 둥근 난형모양의 탈락성 유주포자낭의 특정으로 다른 역병균으로부터 형태적으로 구분되었다. 최적의 생장 온도는 V8 배지와 Oat meal 배지에서의 $25^{\circ}C$이었다. PCR을 통해 ITS rDNA 영역의 약 900 bp의 길이가 증폭되었으며, 세 가지 효소인 Alu I, Msp I, Taq I을 이용한 PCR-RFLP 분석 결과를 PhytID 의 데이터베이스에서 분석한 결과 형태적인 그리고 배양적인 특징에 의한 결과와 일치했다.

Keywords

References

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