Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Free Radical Scavenging Effect and Extraction Condition of Ethanol Extracts and Fractions of Wild Grape Seed (Vitis coignetiea)

머루종자의 적정 추출조건 및 추출 분획물의 Free Radical 소거능 효과

  • Kim, Nan-Young (School of Biotechology and Bioengineering, Kangwon National University) ;
  • Kim, Young-Kuk (School of Biotechology and Bioengineering, Kangwon National University) ;
  • Bae, Ki-Ja (School of Biotechology and Bioengineering, Kangwon National University) ;
  • Choi, Jae-Ho (School of Biotechology and Bioengineering, Kangwon National University) ;
  • Moon, Jea-Hak (Division of Applied Bioscience & Biotechnology, Chonnam National University) ;
  • Park, Geun-Hyung (Division of Applied Bioscience & Biotechnology, Chonnam National University) ;
  • Oh, Deog-Hwan (School of Biotechology and Bioengineering, Kangwon National University)
  • 김난영 (강원대학교 바이오산업공학부) ;
  • 김영국 (강원대학교 바이오산업공학부) ;
  • 배기자 (강원대학교 바이오산업공학부) ;
  • 최재호 (강원대학교 바이오산업공학부) ;
  • 문제학 (전남대학교 응용생물공학부) ;
  • 박근형 (전남대학교 응용생물공학부) ;
  • 오덕환 (강원대학교 바이오산업공학부)
  • Published : 2005.07.01
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Abstract

Wild grape is a traditional medicine plant in north-eastern part of Asia and has been known to have healing properties for various illnesses. This study was to determine the optimum extraction condition and antioxidant activity of ethanol extracts of wild grape (V. coignetiae) seed. Also, organic solvent fractions of hexane, chloroform, ethyl acetate and butanol were obtained from the ethanol extract of wild grape seed at different temperatures. Total ethanol extraction yield of wild grape seed ranged from $4\%\;to\;12\%$ depending on the ethanol concentration, extraction temperature and time condition. The highest extraction yield of $11.9\%$ was obtained at $90\%$ ethanol condition for 12 hour at $70^{\circ}C$. However, the strongest free radical scavenging effect $(RC_{50})$ with $20.93\mu g/mL$ was observed in $70\%$ ethanol extract of wild grape seed extracted for 6 hour at $70^{\circ}C,\;while\;RC_{50}\;with\;40.42$\mu g/mL$ was observed in $90\%$ ethanol extract for 12 hour at $70^{\circ}C$. Antioxidant activity of ethanol extracts of wild grape seed increased as total phenol contents increased. Among each fraction obtained from organic solvents, ethyl acetate fraction was found to have the strongest $RC_{50}\;(8.6\mu g/mL)$ and 636.77 mg GAE/g phenol contents .

식품 산업에서 발생되는 부산물의 가치를 재고하는데 있어서 머루 종자 에탄올 추출물을 대상으로 free radical 소거 능을 탐색하여 머루종자의 기능성을 찾고자 본 실험을 수행하였다. 그 결과, 추출수율은 에탄을 농도, 추출온도 및 시간에 따라 전반적으로 $4\~12\%$를 나타내었으며 $90\%$ 에탄올로 $70^{\circ}$C에서 12시간 추출한 추출물의 수율은 $11.9\%$로 가장 높았지만 free radical 소거능(RC$_{50}$)은 40.42 $\mu$g/mL로 나타났다. 그러나 70$\%$ 에탄올로 $70^{\circ}C$에서 6시간 추출을 하였을 때 추출수율은 $6.9\%$를 나타냈지 만 $RC_{50}$은 $20.93\mu g/mL$로 가장 높았고 총 페놀 함량도 330.71 mg GAE/g으로 가장 높게 나타났다. 본 연구결과, free radical 소거능이 높을수록 총 페놀 함량이 가장 높은 것으로 나타났으며 머루종자 에탄을 추출물의 free radical 소거능이 총 페놀 함량과 밀접하게 관련이 있는 것으로 나타났다. 한편, $70\%$ 에탄올을 $70^{\circ}C$, 6시간 추출물로부터 유기 용매별로 순차적으로 분획한 결과,추출수율은 폴리 페놀 화합물들을 많이 함유하고 있는 ethyl acetate 분획층에서 약 $8\%$로 높았으며, $RC_{50}$도 ethyl acetate 분획층에서 $8.65\mu g/rnL$로 가장 높았고 총 페놀 함량도 ethyl acetate 분획층에서 636.77 mg GAE/g으로 가장 높게 나타났다.

Keywords

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