Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Novel Cationic Microbial Polyglucosamine Biopolymer from New Enterobacter sp. BL-2 and Its Bioflocculation Efficacy

  • SON MI-KYUNG (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • SHIN HYUN-DONG (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • HUH TAE-LIN (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • JANG JIN-HO (Regional Research Center for Processing and Application of Agriculture Products, Daegu University) ;
  • LEE YONG-HYUN (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
  • Published : 2005.06.01
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Abstract

A new bacterium BL-2 excreting a novel cationic polyglucosamine biopolymer was isolated from the spoiled leaves of Chinese cabbage and identified as Enterobacter sp. BL-2. The isolated Enterobacter sp. BL-2 was cultivated in pH-stat fed-batch culture using acetic acid as the feeding stock at pH 8.0, resulting in 17.11 g/l of cells and 1.53 g/l of an extracellular biopolymer after 72 h. The excreted biopolymer was purified by a three-step procedure, involving ethanol precipitation and deproteinizations, to a nearly homogeneous state, and its molecular weight was found to be 106 kDa. It was composed of glucosamine, rhamnose, and galactose at a molar ratio of 86.4:1.6:1.0, respectively, indicating a rarely found novel high-glucosamine-containing biopolymer. The FT-IR and $^{13}C-NMR$ spectra of the novel cationic polyglucosamine biopolymer PGB-l revealed a close identity with chitosan from crab shell. It can effectively flocculate various suspended solids, including kaolin clay, $Ca(OH)_2,\;Al_{2}O_3$, active carbon, microbial cells, and acidic dyes.

Keywords

References

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