Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of Hepatitis C Virus Structural Proteins in Saccharomyces cerevisiae

  • LEE JONG-SOO (BioLeaders Corporation) ;
  • YU JUNG (Laboratory of Infectious Disease, College of Veterinary Medicine, Chungnam National University) ;
  • SHIN HYUN-JIN (BioLeaders Corporation) ;
  • KIM YOUNG-SANG (Department of Biochemistry, Chungnam National University) ;
  • AHN JEONG-KEUN (Microbiology, College of Natural Sciences, Chungnam National University) ;
  • LEE CHONG-KIL (Department of Pharmacy, Chungbuk National University) ;
  • POO HARYOUNG (Proteome Research Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • KIM CHUL-JOONG (Laboratory of Infectious Disease, College of Veterinary Medicine, Chungnam National University)
  • Published : 2005.08.01
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Abstract

Expression in yeast may prove more amenable to generating large amounts of viral antigens for a vaccine candidate. We, therefore, cloned the gene encoding the Hepatitis C virus (HCV) structural proteins (C-El-E2, c740) fused in-frame with, and immediately 3' to, the chicken-lysozyme signal peptide (C-SIG) gene and under the control of the yeast glyceraldehyde-3-phosphate dehydrogenase gene promoter. In yeast, the HCV structural proteins were expressed in two different forms: a processed and a nonprocessed aggregated form. Biophysical characterization by sucrose linear gradient centrifugation revealed that both forms were present in the same fractions with a buoyant density of 1.127-1.176 g/$cm^3$. These findings suggest that the efficient synthesis of HCV structural proteins in yeast may be an important tool to study virus assembly and may lead to the development of an HCV vaccine.

Keywords

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