Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
권호 표지

Immunotoxicological Evaluation of Pollen Intake Using Mice Model

실험동물을 이용한 화분섭취의 면역안전성 평가

  • Park Hee Sung (The Catholic University f Korea, College of Natural Sciences, Department Plant Biotechnology, Kyongsan) ;
  • Heo Young Jeu (The Catholic University f Korea, College of Natural Sciences, Department Plant Biotechnology, Kyongsan) ;
  • Byun Jung-A (Korea Food & Drug Administration, Center for Food Standard Evaluation, Division of Health Supplement Standard) ;
  • Heo Yong (Cathlic University of Daegu, College of National Sciences, Department Occupational Health)
  • 박희성 (대구가톨릭대학교 자연대학 생명공학과) ;
  • 허영주 (대구가톨릭대학교 자연대학 생명공학과) ;
  • 변정아 (식품의약품안전청 식품규격평가부 건강기능식품규격과) ;
  • 허용 (대구가톨릭대학교 자연대학 산업보건학과)
  • Published : 2005.08.01
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Abstract

Pollen has been used for Prevention or treatment of certain diseases such as diabetes, arthritis, or cancer in traditional medicine. In addition, pollen is under investigation as a host cell for a gene expression. This study was undertaken to evaluate the immunologic safety of pollen intake. BALB/c mice were administered with 500, 50,5, or 0.5 mg/kg bw of lily pollen for five times a week for four weeks through gastric intubation. Comparing the control mice administered with distilled water, no significant changes were observed in body weight gain, weight of liver, spleen, lung, and his-topathological findings of liver and kidney of the mice groups administered with the pollen. Plasma level of IgG1, IgG2a, and IgE was not different among the groups. When splenic B lymphocytes were stimulated in vitro with lipopolysaccharides for 7 days, level of IgGl and IgGwa produced in the culture supernatants was not significantly different among the groups. Furthermore, no significant alteration was observed in IL-4 and $IFN{\gamma}$ producing ability with splenic T lymphocytes stimulated in vitro with phytohemagglutinins for 48 hours between the pollen-administered and the control mice. Overall, this study suggests that the lily pollen intake is Inducing no significant modulation of humoral and cell-mediated immunity in mice.

Keywords

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