Effect of Enzymatic Pretreatment on Acid Fermentation of Food Waste

효소 전처리가 음식물 쓰레기의 산발효에 미치는 영향

  • Kim, H.J. (School of Civil and Environmental Engineering, Inha University) ;
  • Kim, S.H. (Department of Civil Engineering, Chosun University) ;
  • Choi, Y.G. (School of Civil and Environmental Engineering, Daegu University)
  • 김희준 (인하대학교 토목환경공학부) ;
  • 김성홍 (조선대학교 토목공학과) ;
  • 최영균 (대구대학교 건설환경공학부)
  • Published : 2005.08.01

Abstract

Food waste can be a valuable carbon source in biological nutrient removal (BNR) systems because of high C/N and C/P ratios. However, food waste should be pretreated to promote its hydrolysis rate because hydrolysis reaction would be a rate-limiting step. This study investigates the influence of the enzymatic pretreatment on acid fermentation of food waste. Solubilization of particulate matter in food waste by using commercial enzymes was examined. The acidification efficiency and the volatile fatty acids (VFAs) production potential of enzymatically pretreated food waste were also examined. The highest volatile suspended solids (VSS) reduction was obtained with an enzyme mixture ratio of 1:2:1 of carbohydrase:protease:lipase. An optimum enzyme dosage for solubilization of food waste was $0.1\%$(V/V) with the enzyme mixture ratio of 1:2:1. In the acid fermentation of enzymatically pretreated food waste, $0.1\%$(V/V) enzyme mixture dosage for pretreatment result in the maximum VFAs production and the best VFAs fraction in soluble COD(SCOD). The VFAs production at this addition level was 3.3 times higher than that of no-enzyme added fermenter. The dominant VFAs present was n-butyrate followed by acetate.

Keywords

References

  1. Choi, E. S., Lee, H. S., Lee, J. W. and Oa, S. W. : Another carbon source for BNR system. Water Science and Technology, 34(1-2), 363-369, 1996
  2. Park, J. A., Kim, S. H. and Hur, J. M. : Nutrient release during the aerobic and alternant aerobic sludge digestion. 한국환경위생학회지, 26(4), 82-87, 2000
  3. Moser-Engeler, R., Udert, K. M., Wild, D. and Siegrist, H. : Products from primary sludge fermentation and their suitability for nutrient removal. Water Science and Technology, 38(1), 265-273, 1998
  4. 김영규, 김인배, 김민호 : 슬러지를 이용한 유기산 발 효 공정의 외부탄소원으로 활용. 한국환경위생학회, 27(4), 79-83, 2001
  5. Lee, S. I., Park, J. H., Ko, K. B. and Koopman, B. : Effect of fermented swine wastes on biological nutrient removal in sequencing batch reactors. Water Research, 31, 1807-1812, 1997 https://doi.org/10.1016/S0043-1354(97)00004-3
  6. Lee, C. Y., Shin, H. S., Chae, S. R., Nam, S. Y. and Paik, B. C. : Nutrient removal using anaerobically fer mented l eachate of f ood waste in t he BNR process. Water Science and Technology, 47(1), 159-165, 2002
  7. Kim, H. J., Kim, S. H., Kim, D. Y. and Chung, T. H. : Effect of alkaline pretreatment on acid fermentation of food waste. In Proc. of the Enviro 2002 and IWA 3rd World Water Congress, Melbourne, 7-12, April, 2002
  8. Barlindhaug, J. and Odegaard, H. : Thermal hydrolysis for the production of carbon source for denitrification. Water Science and Technology, 34(1-2), 371-378, 1996
  9. Stucky, D. C. and McCarty, P. L. : Thermochemical treatment of nitrogenous materials to increase methane yield. Biotechnology and Bioengineering, 8, 219- 233, 1978
  10. Rajan, R. V., Lin, J. G. and Ray, B. T. : Low-level chemical pretreatment for enhanced sludge solubilization. Journal Water Pollution Control Federation, 61, 1678-1683, 1989
  11. American Public Health Association, American Water Works Association and Water Environment Federation : Standard Methods - for the Examination of Water and Wastewater. Ed. by Greenberg, A.C., Clesceri, L.S., Eaton, A.D. 18th Edition, APHA, 1992
  12. Higgins, M. J. and Novak, J. T. : Characterization of exocellular protein and its role in bioflocculation. Journal of Environmental Engineering, 127, 479-485, 1997
  13. Parmar, N., Singh, A. and Ward, O. P. : Enzyme treatment to reduce solids and improve setting of sewage sludge. Journal of Industrial Microbiology and Biotechnology, 26, 383-386, 2001 https://doi.org/10.1038/sj.jim.7000150
  14. Eastman, J. A. and Ferguson, J. F. : Solubilization of particulate organic carbon during the acid phase of anaerobic digestion. Journal Water Pollution Control Federation, 53, 352-366, 1981
  15. Zoetemeyer , R. J ., van den Heuvel, J . C. and Cohen, A. : pH influence on acidogenic dissimilation of glucose in an anaerobic digestor. Water Research, 16, 303-311, 1982 https://doi.org/10.1016/0043-1354(82)90190-7
  16. Argelier, A., Delgenes, J. Ph. and Moletta, R. : Design of acidogenic reactors for the anaerobic treatment of the organic fraction of solid food waste. Bioprocess Engineering, 18, 309-315, 1998 https://doi.org/10.1007/s004490050448