Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of Multiple Superovulation and Parity on Embryo Production in Hanwoo

한우의 반복 과배란 및 산차가 수정란 생산에 미치는 영향

  • Choi S. H. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Ryu I. S. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Son D. S. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Cho S. R. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Han M. H. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Kim H. J. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Choe C. Y. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Kim Y. K. (Animal Genetic Resources Station, NLRI, RDA)
  • 최선호 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 류일선 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 손동수 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 조상래 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 한만희 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 김현종 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 최창용 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 김영근 (농촌진흥청 축산연구소 가축유전자원시험장)
  • Published : 2005.08.01
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Abstract

This study was performed to investigate the effects of multiple superovulation and parity on embryo production in Hanwoo cows. Donors were superovulate 4 times $1\~2$ months interval and inserted CIDR plus (with the capsule of estradiol benzoate 10mg) on Day 10 from standing heat for 9 days and injected 2.5ml FSH (Antorin R-10) 2 times in a day on 6th day to 10th day from insertion of CIDR and the doses of FSH were decreasing 0.5ml on every 2 times. On 3th day of FSH injection, 25ml $PGF_2{\alpha}$ were injected i.m. and on 4th day, CIDR was removed. After 2 days from removing CIDR, AI was performed 2 times 12 hour apart with 2 straws of Korean Proved frozen Semen and simultaneously 200ug/ml GnRH was injected and embryos were recovered on 7th day from Al. The response rates of superovulated donors were $85.7\%,\;90.5\%,\;62.5\%,\;100\%$ from 1 to 4 times of superovulation, respectively. There were significant differences among No. of superovulation times (P<0.05). The results of transferable embryos were 3.7, 3.4, 3.4, 5.7 from 1 to 4 times of superovualtion, respectively. There were no differences among No. of superovulation times. The results of transferable embryos were 2.5, 3.0, 5.3, 3.0, 3.4 form heifer, first born to 4 the born, respectively. There were significant differences among the parities of donors (P<0.05). These results suggested that even 4 times of superovulations of Hanwoo donors could be able to recover transferable embryos, it might be used the donors maximally and improved the adaptation of embryo transfer to farms safely.

본 연구는 소에 있어서 다량의 수정란을 생산하여, 고능력의 유전인자를 조기에 확산할 수 있도록, 한우 공란우의 반복 과배란을 유도하여 안정적이고 효율적인 수정란 생산을 목적으로 실시하였으며, 그 결과는 다음과 같다. 1. 한우 경산우 및 미경산우를 4회 반복적으로 과배란 처리하였을 때, 수정란 채란이 가능한 공란우는 $85.7\%,\;90.5\%,\;62.5\%,\; 100\%$였으며, 3회 처리시 다소 낮아, 약간의 유의차가 인정되었다(P<0.05). 2. 한우 경산우 및 미경산우를 4회 반복적으로 과배란 처리하였을 때, 수정란 회수는 각각 9.2개, 9.9개, 7.0개, 10.7개였으며, 이식 가능 수정란은 3.7개, 3.4개, 3.4개, 5.7개였다. 반복과배란 처리에 의한 이식 가능 수정란의 회수 율은 유의차가 인정되지 않았다. 3. 한우 공란우의 산차에 따른 수정란의 회수는 미경산우 6.0개였으며, $1\~4$산까지는 각각 8.7개, 11.4개, 9.9개, 7.6개였으며, 이식 가능 수정 란은 2.5개, 3.0개, 5.3개, 3.0개, 3.4개로 나타났으며, 미경산 및 1산차에서 약간 낮은 수준이었다(P<0.05). 이상의 결과로 한우 공란우는 경산우 및 미경산 우를 4회 연속 과배란 처리하여 수정란을 채취하여도 적정량의 수정란을 생산할 수 있었으며, 산차에 의한 수정란회수율에 있어서도 적정량의 수정란을 회수할 수 있으므로, 고능력의 한우 공란우를 알맞은 번식 및 사양관리를 통하여 유전능력을 최대한 이용할 수 있어, 수정란 이식 기술의 산업화를 안정적으로 시행할 수 있을 것으로 기대된다.

Keywords

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