Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Identification of MC1R gene variants of Hanwoo and Holstein meat using PCR-RFLP

PCR-RFLP를 이용한 한우와 젖소고기의 MC1R 유전자변이 검출

  • Koh Ba-Ra-Da (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Kim Yong-Hwan (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Park Seong-Do (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Na Ho-Myung (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Kim Jeong-Nam (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Sung Chang-Min (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Lee Sam-Soo (Gwangju Metropolitan Health & Environment Research Institute)
  • 고바라다 (광주광역시 보건환경연구원) ;
  • 김용환 (광주광역시 보건환경연구원) ;
  • 박성도 (광주광역시 보건환경연구원) ;
  • 나호명 (광주광역시 보건환경연구원) ;
  • 김정남 (광주광역시 보건환경연구원) ;
  • 성창민 (광주광역시 보건환경연구원) ;
  • 이삼수 (광주광역시 보건환경연구원)
  • Published : 2005.09.01
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Abstract

The melanocortin 1 receptor (MC1R) encoded by the coat color extension gene (E) plays a key role in the signaling pathway of melanin synthesis. The primers for the amplification of bovine MC1R gene were designed based on a bovine MC1R gene sequence (GenBank accession no. Y19103). A size of 483bp (482bp for Hanwoo) was amplified by PCR, digested with Hpa II restriction enzyme and electrophoresed in $1.5\%$ agarose gel. When the amplified DNA product (483 bp) was digested with Hpa II restriction enzyme, Hanwoo meat showed a single band of 482bp, whereas two fragments of 325bp and 158bp were detected in Holstein, Angus and meat of Hanwoo / Holstein cross cow having back coat color phenotype, respectively. The results of this experiment Indicate that new designed primers of bovine MCIR gene may be useful for identification of Hanwoo meat from Holstein, Black Angus and Hanwoo / Holstein cross cow meat.

Keywords

References

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