The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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The Effect of Fungicide Carbendazim on Hepatic detoxication systems of rat

살균제 carbendazim이 랫드 간 해독체계에 미치는 영향

  • Lee, Je-Bong (National Institute of Agricultural Science and Technology) ;
  • Shin, Jin-Sup (National Institute of Agricultural Science and Technology) ;
  • Jeong, Mi-Hye (National Institute of Agricultural Science and Technology) ;
  • Park, Yeon-Ki (National Institute of Agricultural Science and Technology) ;
  • Kang, Kyu-Young (Dept. of Environ. Biotechnology, Gyeong Sang National University)
  • 이제봉 (농촌진흥정 농업과학기술원 농약안전성과) ;
  • 신진섭 (농촌진흥정 농업과학기술원 농약안전성과) ;
  • 정미혜 (농촌진흥정 농업과학기술원 농약안전성과) ;
  • 박연기 (농촌진흥정 농업과학기술원 농약안전성과) ;
  • 강규영 (경상대학교 환경생명화학과)
  • Published : 2005.12.30
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Abstract

Serum alanine aminotransferase(ALT), aspartate aminotransferase (AST), hepatic glutathione, glutathione S-transferase(GST), cytochrome P450 and cytochrome P450 reductase activity were measured to investigate the effects of hepatic detoxication system and metabolic activities of carbendazim in Sprague Dawley(S.D.) male rat at dose levels of 375, 750 or 1,500 mg/kg body weight. Serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) activities were slightly increased in all test groups after 120 minutes of administration. Glutathione was increased about 20% at high and medium dose level within 120 minutes after administration, while activity of glutathione S-transferase was decreased $36{\sim}50%$. However, the enzyme activity was recovered from all test groups after 240 minutes of administration. Cytochrome P450 and activity of cytochrome P450 reductase were decreased $25{\sim}50%$ until 120 minutes after administration, but recovered after 240 minutes.

Carbendazim의 랫드 간 해독체계에 대한 영향을 검색하기 위하여 간장독성의 지표 효소인 혈장 ALT(alanine aminotransferase) 및 AST(aspartate aminotransferase) 활성, 간에 대한 독성, 해독 및 대사에 대한 영향을 구명하기 위하여 간 GSH(glutathione), GST(glutathione-S-transferase), cytochrome P450 및 cytochrome P450 reductase 활성을 375, 750, 1,500 mg/kg 약량에서 측정한 결과 혈장 ALT 및 AST 활성이 120분 후에 약간의 증가가 있었으나 특이한 독성증상은 관찰되지 않았다. GSH는 고농도와 중농도 120분에서 20%의 함량증가가 있었고, GST는 120분까지 $36{\sim}50%$ 정도의 활성저해가 있었으나 240분에는 활성이 회복되었다. Cytochrome P450함량은 60분까지 $25{\sim}50%$까지 함량이 저하되었으나 120분에 70%이상 회복되었고, 240분 저농도에서는 거의 회복되었으며, cytochrome P450 reductase도 120분까지 $25{\sim}50%$ 활성저해가 있었으나, 240분에는 무처리 군의 활성과 유사하게 회복되어 대사관련 효소에 대한 손상은 크지 않을 것으로 판단되었다. 이상의 결과에서 benzimidazole 계 살균제인 carbendazim은 생체내 해독 및 대사관련 체계에 영향이 적은 농약으로 급성적인 중독은 일으키지 않을 것으로 판단되었다.

Keywords

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