The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Suppression of Meloidogyne arenaria by different treatments of Pasteuria penetrans

Pasteuria penetrans의 처리방법에 따른 땅콩뿌리혹선충 (Meloidogyne arenaria) 방제효과

  • Zhu, Yong-Zhe (Laiyang Agricultural College) ;
  • Park, Dong-Sik (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Cho, Myoung-Rae (Rural Development Adminstration, National Institute of Highland Agriculture) ;
  • Hur, Jang-Hyun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Lim, Chun-Keun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
  • ;
  • 박동식 (강원대학교 농업생명과학대학 자원생물환경학과) ;
  • 조명래 (농촌진흥청 고령지농업연구소) ;
  • 허장현 (강원대학교 농업생명과학대학 자원생물환경학과) ;
  • 임춘근 (강원대학교 농업생명과학대학 생물자원공학부)
  • Published : 2005.12.30
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Abstract

This study was investigated to compare the suppression of Meloidogyne arenaria by different treatments of Pasteuria penetrans which is known for biological control agent against Meloidogyne spp.. In order to select proper number of P. penetrans showing good suppression effect, P. penetrans were mixed with M, arenaria for attachment using three different concentration such as $3{\times}10^4$, $3{\times}10^5$ and $3{\times}10^6$ endospores/5 g medium, followed by treating them onto the roots of tomato. After 14 weeks incubation, P. penetrans at $3{\times}10^6$ endospores showed highest activity against the formation of gall caused by M, arenaria. At a dose of $3{\times}10^5$ endospores/5 g medium, P. penetrans was treated into soil either mixing with soil or spray onto soil surface for comparing of suppressive efficacy. When the antagonistic bacterium was treated by the former method, it suppressed more effectively Using P. penetrans at $3{\times}10^6$ endospores and mixing with soil method, suppression was compared among P. penetrans, $PASTORIA^{(R)}$(Japan) and $Fosthiazate^{(R)}$(Korea). P. penetrans was more potent than $PASTORIA^{(R)}$(Japan) and as similar as $Fosthiazate^{(R)}$(Korea). Therefore, these results suggested that P. penetrans can be used for controling of M. arenaria as biological control agent. Furthermore, thess results can be provided to develop environmentally-friendly nematicide.

본 연구는 국내토착 토양세균인 Pasteuria penetrans 내생포자의 처리방법에 따른 땅콩뿌리혹선충(Meloidogyne arenaria) 방제효과를 파악하고자 수행하였다. 이를 위해 P. penetrans 내생포자의 농도($3{\times}10^4$, $3{\times}10^5$ 및 $3{\times}10^6$ endospores/5 g medium)별로 500마리 땅콩뿌리혹선충에 각각 부착한 후 기주식물인 토마토 뿌리에 접종하여 14주 후 뿌리에 형성된 뿌리혹 개체 수로 농도에 따른 선충방제 효과를 파악하였다. 그 결과 80%이상 방제효과를 보인 $3{\times}10^5$ 수준의 P. penetrans 내생포자를 500마리의 선충만을 접종한 토양과 혼합하여 처리한 시험구(토양혼화처리)와 토양표면에 처리한 시험구(토양표면처 리)로 구분하여 방제효과를 비교하였다. 이 중 방제효과가 높았던 토양혼화처리 방법을 이용하여 P. penetrans와 일본 상품인 $PASTORIA^{(R)}$그리고 국내에 등록된 살선충제인 선충탄(fosthiazate)과의 방제효과를 상호 비교하였다. 실험결과 P. penetrans의 농도가 높고 토양과 혼합하여 처리할 시 방제효과가 높았으며, P. penetrans 처리가 일본 제품보다는 우수한 방제효과를 보였고 국내 시판 화학방제제와는 유사하였다.

Keywords

References

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