Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Electrochemical Reduction of Xylose to Xylitol by Whole Cells or Crude Enzyme of Candida peltata

  • Park Sun Mi (Department of Biological Engineering, Seokyeong University) ;
  • Sang Byung In (Division of Water Environment and Remediation, KIST) ;
  • Park Dae Won (Department of Energy and Environmental Engineering, Seoul National University of Technology) ;
  • Park Doo Hyun (Department of Biological Engineering, Seokyeong University)
  • Published : 2005.10.01
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Abstract

In this study, whole cells and a crude enzyme of Candida peltata were applied to an electrochemical bioreactor, in order to induce an increment of the reduction of xylose to xylitol. Neutral red was utilized as an electron mediator in the whole cell reactor, and a graphite-Mn(IV) electrode was used as a catalyst in the enzyme reactor in order to induce the electrochemical reduction of $NAD^+$ to NADH. The efficiency with which xylose was converted to xylitol in the electrochemical bioreactor was five times higher than that in the conventional bioreactor, when whole cells were employed as a biocatalyst. Meanwhile, the xylose to xylitol reduction efficiency in the enzyme reactor using the graphite-Mn (IV) electrode and $NAD^+$ was twice as high as that observed in the conventional bioreactor which utilized NADH as a reducing power. In order to use the graphite-Mn(IV) electrode as a catalyst for the reduction of $NAD^+$ to NADH, a bioelectrocatalyst was engineered, namely, oxidoreductase (e.g. xylose reductase). $NAD^+$ can function in this biotransformation procedure without any electron mediator or a second oxidoreductase for $NAD^+/NADH$ recycling

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References

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