Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Histochemical Study of the Intestinal Mucosubstances in Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus and Takifugu pardalis

조피볼락, 용치놀래기, 송곳니베도라치 및 졸복 장관 점액질에 대한 조직화학적 연구

  • Jeong Cil-Nam (Department of Biology Education, Pusan National University) ;
  • ;
  • ;
  • 정길남 (부산대학교 사범대학 생물교육과) ;
  • 조기진 (부산대학교 사범대학 생물교육과) ;
  • 이응희 (부산대학교 사범대학 생물교육과) ;
  • 조운복 (부산대학교 사범대학 생물교육과)
  • Published : 2005.10.01
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Abstract

The conventional histochemical staining were used to study mucosubstances properties of intestinal striated border and goblet cells in four teleostean species, i. e., Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis. The following methods were used; PAS, AB pH 2.5, AB pH 1.0, AB pH 2.5-PAS, AF pH 1.7-AB pH 2.5 and HID-AB pH 2.5 stains. The mucosubstances of striated border in the proximal intestine and rectum of Sebastes schlegeli contained with neutral mucin, middle and distal intestine contained with neutral mucin and acid mucin. The striated border of all the intestines of Halichoeres poecilopterus contained with neutral mucin and acid mucin, and those of Bryzoichthys lysimus and Tnkifugu pardalis contained with neutral mucin only. The amounts of neutral mucin were moderate to considerable in Sebastes schlegeli and Halichoeres poecilopterus, minimal to small in Bryzoichthys lysimus and Tnkifugu pardalis. The amounts and properties in mucosubstances of intestinal goblet cells showed differences in species and regions. The intestinal goblet cells of Bryzoichthys lysimus, and Tnkifugu pardalis contained neutral mucin only while Sehastes schlegeli and Halichoeres poecilopterus contained mixture of neutral mucin, sulfomucin and sialomucin. The amounts of neutral mucin were considerable to large in distal intestine and rectum of Tnkifugu pardalis, while moderate to considerable in all intestines of Sehastes schlegeli, all the intestines except for middle intestine of Bryzoichthys lysimus, and proximal and middle intestine of Tnkifugu pardalis. Also it was minimal to small in middle intestine of Halichoeres poecilopterus. The intestinal goblet cells of Sehastes schlegeli contained mixture of minimal amounts of strong sulfmucin, weak sulfomucin and minimal to small amounts of sialomucin, and those of Halichoeres poecilopterus except for rectum contained mixture of minimal to small amounts of strong sulfomucin and sialomucin.

경골어류 4종(조피볼락, 용치놀래기, 송곳니베도라치 및 졸복)의 장관 상피 선조연 및 배상세포의 점액질의 조직화학적 성상을 밝히기 위해 PAS 반응, AB pH 1.0 및 pH 2.5, AB pH 2.5-PAS, AF pH 1.7-AB pH 2.5 및 HID-AB pH 2.5 염색법을 사용하였다. 장관 선조연의 점액질은 조피볼락의 근위장과 직장은 중성점액질만을, 중간장과 원위장은 중성점액질과 산성점액질의 혼합성이었으나 용치놀래기는 전 장관에서 중성점액질과 산성점액질을 함유하고 있었으며, 송곳니베도라치와 졸복의 전 장관은 중성점액질만을 함유하고 있었다. 중성점액질의 양은 조피볼락과 용치놀래기는 중등량 내지 상당량, 송곳니베도라치와 졸복은 미량 내지 소량이었다. 장 배상세포 점액질의 양과 성상은 어종 및 장 부위에 따라 차이가 있어 송곳니베도라치와 졸복은 중성점액질만을 함유하고 조피볼락과 용치놀래기는 중성 점액질, sulfomucin 및 sialomucin의 혼합성이었다. 중성점액질의 양은 졸복의 원위장 및 직장은 상당량 내지 다량이었고 조피볼락의 전장, 용치놀래기의 근위장, 원위장 및 직장, 졸복의 근위장 및 중간장은 중등량 내지 상당량이었으며 용치놀래기의 중간장은 미량 내지 소량이었다. 조피볼락의 장 배상세포는 미량의 강 sulfomucin, 약 sulfomucin 및 미량 내지 소량의 sialomucin을 함유하고 있었으며 직장을 제외한 용치놀래기의 장 배상세포는 미량 내지 소량의 강 sulfomucin과 sialomucin을 함유하고 있었다.

Keywords

References

  1. Allen, A, D. A Hutton, A J. Leonard, J. P. Pearson and J. A Sellers. 1986. The role of mucus in the protection of the gastroduodenal mucosa. Scand J. Gastroenterol. 21(suppI. 125), 71-77
  2. Anderson, T. A. 1986. Histological and cytological structure of the gastrointestinal tract of the luderick, Girella tricuspidata in relation to diet, J. Morp. 190, 109-119 https://doi.org/10.1002/jmor.1051900110
  3. Andrew, W. and C. P. Hickman. 1974. Histology of the vertebrates. Mosby Co., Saint Louis, pp. 70
  4. Bucke, D. 1971. The anatomy and histology of the alimentary tract of the carnivorous fish, the pike, Esox lucius. J. Fish BioI. 3, 421 https://doi.org/10.1111/j.1095-8649.1971.tb05914.x
  5. Chakrabarti, P., S. Ganguly and D. K. MandaI. 1994. Histochemical studies of the digestive system of freshwater cichlid fish, Oreochromis mossambicus (Peters). J. Freshwat. 6, 63-69
  6. Chan, V. M. 1941. The histology of the alimentary tract of the deep water gumard, Peristedion longispatha. Univ. Nebraska. Stu. Sci. Tech. 1, 7-30
  7. Curry, E. 1939. The histology of the digestive tube of the carp, Cyprinus carpio communis). J. Morp. 65, 53-78 https://doi.org/10.1002/jmor.1050650104
  8. Eversole, J. R. 1972. The mucoprotein histochemistry of human mucous acinar cell containing salivary glands : submandibular and sublingual glands. Arch, Biol. 17, 4354
  9. Gutierrez, M. and M. C. Sarasquete. 1986. Distribuci6n histoquimica de carbohidratos y proteinas en est6mago e intestino de Anguilla anguilla L., 1758 (Osteichthyes, Anguillidae) de las salinas de Cadiz, lav. Preq. 50, 553-564
  10. Huebner, E. and G. Chee. 1978. Histological and ultrastructural specialization of the digestive tract of the intestinal air breather in Hoplosternum thoracatum (Teleost). J. Morp. 157, 301-328 https://doi.org/10.1002/jmor.1051570305
  11. Jabom, A, J. C. Olsson, A Westerdahl, P. L. Conway and S. Kjelleberg. 1997. Colonization in the fish intestinal tract and production of inhibitory substances in intestinal mucus and faecal extracts by Carnobacterium sp. strain K1. Journal of Fish Diseases 20, 383-392 https://doi.org/10.1046/j.1365-2761.1997.00316.x
  12. Johnson, W. C., F. B. Johnson and E. B. Herwig. 1962. Effect of varying the pH on reaction for acid mucopolysaccharides. J. Histochem. Cytochem. 10, 684
  13. Krementz, A B. and G. B. Chapman. 1975. Ultrastructure of the posterior half of the intestine of the Channel Catfish, lctalurus punctatus. J. Morp. 145, 441-482 https://doi.org/10.1002/jmor.1051450405
  14. Lev, R. and S. S. Spicer. 1964. Specific staining of sulfate groups with alcian blue at low pH. J. Histochem. Cytochem. 12, 309 https://doi.org/10.1177/12.4.309
  15. McManus, J. F. A 1968. Periodic acid Schiff's reaction. Stain Techn., 23; 99-108, 1948 (AFlP modification). Copyright by manual of histologic staining methods of the armed forces institute of pathology. 3rd ed. pp. 160
  16. Mowry, R. W. 1956. Alcian blue technics for the histochemical study of acidic carbohydrates. J. Histochem. Cytochem. 4, 407
  17. Neutra, M. R. and J. F. Forstner. 1987. Gastrointestinal mucus; Synthesis, secretion and function. In: Physiology of the gastrointestinal tract. Johnson, J. D. ed. Raven Press, New York, pp. 975-1009
  18. Parillo F., A M. Gargiulo and O. Fagioli. 2004. Complex Carbohydrates Occurring in the Digestive Apparatus of Umbrina Cirrosa (L.) Fry. Veterinary Research Communications 28, 267-278 https://doi.org/10.1023/B:VERC.0000026671.88218.08
  19. Prasad, T. N., D. K. Sahu and A Moitra. 1992. Histochemical observations on the nature of mucous secreting cells in the gastrointestinal tract of Channa punctatus. J. Frewater 4, 267-272
  20. Reifel, C. W. and A. A. Travill. 1979. Structure and carbohydrate histochemistry of the intestine in ten teleostean species. J. Morp. 162, 343-360 https://doi.org/10.1002/jmor.1051620305
  21. Sheahan, D. G. and H. R. Jervis. 1976. Comparative histochemistry of gastrointestinal mucosubstances. Am. J. Anat. 146, 103-117 https://doi.org/10.1002/aja.1001460202
  22. Spicer, S. S. and D. B. Meyer. 1960. Histochemical differ.entiation of add mucopolysaccharides by means of combined aldehyde fuchsin-aldan blue staining. Am. J. Clin. path. 33, 453-460 https://doi.org/10.1093/ajcp/33.5_ts.453
  23. Spicer, S. S. 1965. Diamine methods for differentiating mucosubstances histochemically. J. Histochem. Cytochem. 13(3), 211-234 https://doi.org/10.1177/13.3.211
  24. Suganuma, T. T., M. Katsuyarna, M. Tsukahala, Y. Tatematsu, Y. Sakakura and F. Murata. 1981. Comparative Histochemical study of alimentary tracts, with special reference to the mucous neck cells of the stomach. Am. J. Anat. 161, 219-238 https://doi.org/10.1002/aja.1001610206
  25. Yamada, K. 1974. The effect of digestion with chondroitinases upon certain cytochemical reactions of mucopolysaccharidecontaining tissue. J. Histochem. Cytochem. 22, 266-275 https://doi.org/10.1177/22.4.266
  26. 김용국. 1970. 담수어류의 소화관 점막에 관한 비교조직화학적 연구. 부산대학교 논문집 14, 135-160
  27. 변경애, 조운복. 1985. 감성돔. 퉁쏠치 및 볼락 장관 점액질의 조직화학적 성상. 부산대학교 자연과학 논문집 40, 251-269
  28. 조운복, 김봉선, 최인장, 백선용, 신인성. 1984. 경골어 장관 점액세포내 점액질의 조직화학적 성상. 부산대학교 자연과학 논문집 37, 318-327
  29. 조운복, 박해춘. 1972. 양서류 및 어류의 소화기관 점막에 관한 비교 조직화학적 연구. 부산대학교 논문집, 13, 383-403
  30. 조운복. 1974. 하동척추동물 소화관 점막내 산성점액질의 조직화학적 성상에 관한 연구. 대한해부학회지 7, 19-30