Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Screening and Extraction Condition of Antiaging Bioactive Substances from Medicinal Plants

각종 약용 식물로부터 노화 억제 관련 생리활성 물질의 탐색 및 추출 조건

  • Yu, Hyung-Eun (Dept. of Life Science and Genetic Engineering, Paichai Univerity) ;
  • Dela Paz, Leaniza Michella M. (Dept. of Life Science and Genetic Engineering, Paichai Univerity) ;
  • Bae, Young-Joo (Dept. of Life Science and Genetic Engineering, Paichai Univerity) ;
  • Lee, Dae-Hyoung (Dept. of Life Science and Genetic Engineering, Paichai Univerity) ;
  • Park, Jong-Sang (Central Research Institute, Pan Korea Co.) ;
  • Kwak, Hahn-Shik (Dept. of Life Science and Genetic Engineering, Paichai Univerity) ;
  • Kim, Ha-Kun (Dept. of Life Science and Genetic Engineering, Paichai Univerity) ;
  • Lee, Jong-Soo (Dept. of Life Science and Genetic Engineering, Paichai Univerity)
  • 유형은 (배재대학교 과학기술 바이오대학 생명유전공학과) ;
  • ;
  • 배영주 (배재대학교 과학기술 바이오대학 생명유전공학과) ;
  • 이대형 (배재대학교 과학기술 바이오대학 생명유전공학과) ;
  • 박종상 ((주)판코리아 중앙 연구소) ;
  • 곽한식 (배재대학교 과학기술 바이오대학 생명유전공학과) ;
  • 김하근 (배재대학교 과학기술 바이오대학 생명유전공학과) ;
  • 이종수 (배재대학교 과학기술 바이오대학 생명유전공학과)
  • Published : 2005.10.01
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Abstract

To develop new anti-aging foods or cosmetics by using antioxidants, SOD activator and elastase inhibitor, both potent anti-aging substances, were screened from various extracts of medicinal Plants and its optimal extraction conditions were investigated. Antioxidant activity has showed the highest in methanol extracts of Prunus persica (seed; 98.0$\%$). Methanol extracts of Morus alba (leave; 41.0$\%$) showed the highest elastase inhibitory activity while Lycium chinense (fruit; 197$\%$) showed the highest activation effect in SOD activity. The Prunus persica extract that exhibited the highest activity was extracted by treatment of Prunus persica powder with methanol at 40$^{\circ}C$ for 18 h and the SOD activity was maximum with extract from Lycium chinense extracted with deionized water at 30$^{\circ}C$ for 12 h. Elastase inhibitory activity of Morus alba was maximally extracted when it was treated with 70$\%$ methanol at 50$^{\circ}C$ for 12 h.

본 연구에서는 비교적 가격이 저렴하고 부작용이 없으면서 효능이 우수한 노화 억제 관련 생리 기능성 제품을 개발하고자 먼저 다양한 종류의 약용식물을 대상으로 물과 메탄올 추출물을 제조한 후 이들의 항산화 활성, SOD 활성과 elas-tase저해 활성을 측정하여 우수 약용식물을 선발한 후 이들로부터 노화 억제 관련 생리 활성 물질들의 추출 최적 조건을 검토하였다. 124종의 약용식물에 대한 물과 메탄올 추출물 가운데 항산화 활성은 도인(Prunus persica)의 메탄올 추출물이 98.4$\%$로 가장 높았고, SOD 활성은 구기자(Lycium chinense)의 물 추출물이 197$\%$로, elastase 저해활성은 상엽(Morus alba) 메탄올 추출물이 41$\%$로 가장 높았다 또한 항산화물질은 도인(Prunus persica)을 메탄올로 1 :20 현탁시킨 후 40$^{\circ}C$, 12시간 추출하였을 때 가장 많이 추출되었고 SOD 활성 물질은 구기자(Lycium chinense)를 1:30의 물로 30$^{\circ}C$, 12시간, elastase 저해 물질은 상엽 (morus alba)을 메탄올로 50$^{\circ}C$에서 12시간 진탕시켰을 때 가장 많이 추출되었다.

Keywords

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