Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Ethanol Extract of Antler Velvet Attenuates Testicular Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD ) in Rats

랫드에 있어 녹용 알콜 추출물의 TCDD-유발 고환 독성 방어 효과

  • Choi, Kyung-Yun (Dept. of Life Science, College of Biomedical & Health Science, Konkuk University) ;
  • Hwang, Seock-Yeon (Dept. of Pathology, Chungbuk National University Hospital) ;
  • Kim, Si-Kwan (Dept. of Life Science, College of Biomedical & Health Science, Konkuk University)
  • 최경운 (건국대학교 의료생명대학 생명과학부) ;
  • 황석연 (충북대학교 의과대학 부속병원 임상병리실) ;
  • 김시관 (건국대학교 의료생명대학 생명과학부)
  • Published : 2005.10.01
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Abstract

This study was carried out to investigate the biological effect of deer's antler velvet on the testicular toxicity of rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD ). Thirty male rats were divided into three equal groups. The control group received vehicle (DMSO/acetone/soybean oil mixture) and saline; single dose of 50 $/mu$g/kg body weight TCDD was injected intraperitoneally into the single TCDD-treated and test group. Test group received ethanol extract of antler velvet (EAV) at daily dose of 20 mg/kg body weight for 5 weeks from one week before TCDD exposure. Decrease in body weight increment was less remarkable in test group compared with that of TCDD-treated group. TCDD-induced decrease in testicular weight, microtubular diameter and Johnson's score, and lesion were significantly alleviated by the treatment of EAV. This result led us to the conclusion that antler velvet can attenuate TCDD-induced testicular toxicity in rats.

본 연구는 다이옥신(2,3,7,8-tetrachlorodibenzo-P-dioxin, TCDD)에 노출된 랫드에 있어 녹용의 고환 독성에 대한 방어 효과를 조사하기 위하여 수행되었다. 실험은 30마리의 수컷 랫드를 10마리씩 3개군으로 나누어 정상대조군(CO)에는 vehicle을 TCDD-단독투여군(TA)과 녹용 에탄올 추출물(EAV) 투여군(TE)에는 50 $\mu$g/kg body weight의 다이옥신을 일회 복강내 주사하였다. TE는 다이옥신 노출 1주 전부터 녹용 에탄올 추출물을 20 mg/kg body weight씩 5주 동안 매일 복강 주사하였다 TA에서는 다이옥신에 의한 체중감소(CO 대비 61.1$\%$)가 현저하였으며 TE 역시 체중은 감소(CO 대비 77.0$\%$)하였으나 YA와 비교해 볼 때 그 정도는 유의하게 경감되 는 것을 알 수 있었다. 다이옥신(TCDD)에 의하여 야기되는 고환 중량, 정세관의 직경 및 Johnsen's score의 감소와 고환조직의 병변은 녹용 에탄올 추출물 투여에 의하여 유의하게 경감되었다. 이상의 결과로 보아 녹용은 랫드에 있어 다이옥신에 의하여 야기되는 고환 독성을 경감시킬 수 있다고 판단한다.

Keywords

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