Entomological Research

The Entomological Society of Korea (한국곤충학회)
권호 표지

Inhibitory Effect of Silkworm - Extract (SE) on Monoamine Oxidase Activity in Vitro and in Vivo

  • KANG Young-Kook (Department of Biology, Daejeon University) ;
  • NAM Sang-Ho (Department of Biology, Daejeon University) ;
  • SOHN Hyung-Ok (Laboratory of Biochemistry, KT&G Central Research Institute) ;
  • LEE Dong-Wook (Laboratory of Biochemistry, KT&G Central Research Institute)
  • Published : 2005.09.01
⟨ Previous Next ⟩

Abstract

In vitro, MAO-A activity was inhibited $16-25\%$, and MAO-B activity was inhibited $20-50\%$ by SE treatment (12.5, 25 and $50{mu}g$). In vivo, male C57BL/6 mice received intraperitoneal injection of SE (20 mg/kg/day) for 14 days. The results showed that MAO-A activity of pre-SE-treatment mice brain was inhibited in whole brain, cerebral cortex, substantia nigra. MAO- B activity of pre-SE-treatment mice brain was inhibited in substantia nigra and cerebellum than saline-treated control group. These results suggest that SE inhibits MAO activity in vivo, which would be expected to results in anti -depressive and neuroprotective effects.

Keywords

References

  1. Ahmad, M., S. Saleem, A.S. Ahmad, S. Yousuf, M.A. Ansari, M.B. Khan, T. Ishrat, R.K. Chaturvedi, A.K. Agrawal, and F. Islam, 2005. Ginkgo biloba affords dose-dependent protection against 6-hydroxydopamininduced parkinsonism in rats: neurobehavioural, neurochemical and immunohistochemical evidences. J. of Neurochemistry. 93: 94-104 https://doi.org/10.1111/j.1471-4159.2005.03000.x
  2. Bach, H.W.J., N.C. Lan, D.L. Johnson, C.W. Abell, M.E. Bembenek, S.W. Kwan, P.H. Seeburg, and J.C. Shih, 1988. DNA cloning of human liver monoamine oxidase A and B; molecular basis of differences in enzymatic properties. Proc. Natl. Acad. Sci. USA. 85: 4934-4938
  3. Chung, S.H., J.H. Ryu, and I.J. Kim, 1996. Blood glucose lowering of silkworm. J. HyungHee Pharm. 24: 95-100
  4. Foley, P., M. Gerlach, M.B.H. Youdim, and P. Rieder, 2000. MAO-B inhibitors: multiple roles in the therapy of neurodegenerative disorders? Parkinsonism and Related Disorders. 6: 25-47 https://doi.org/10.1016/S1353-8020(99)00043-7
  5. Fujikawa, T., S. Miguchi, N. Kanada, N. Nakai, M. Ogata, I. Suzuki and K. Nakashima, 2005. Acanthopanax senticosus Harms as a prophylactic for MPTP-induced Parkinson's diseases in rats. J. of Ethnopharmacology. 97: 375-381 https://doi.org/10.1016/j.jep.2004.11.031
  6. Kalaria, R.N., M.J. Mitchell, and S.I. Harik, 1987. Correlation of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine neurotoxicity with blood brain barrier monoamine oxidase activity. Proc. Natl. Acad. Sci. USA. 84: 3521- 3525
  7. Kampen, J.V., H. Robertson, T. Hagg, and R. Drobitch, 2003. Neuroprotective actions of the ginseng extract G115 in two rodent models of Parkinson's disease. Experimental Neurology. 184: 521-529 https://doi.org/10.1016/j.expneurol.2003.08.002
  8. Kang, Y.K., H.B. Lim, H.O. Sohn, Y.G. Lee, D.W. Lee, and S.H. Nam, 2000. Effect of silkworm-extract on mucus secretion in rat tracheobronchial lumen. Kor. J. Entomol. 30(2): 71-75
  9. Kang, Y.K., SK Choo, and SH Nam, 2001. Effects of silkworm- extract on blood component's levels in mice. Korean J. of Ent. 31(4): 243-247
  10. Kim, S.H., K.S. Kim, J.H. Lee, E.K. Chung, Y.S. Park, Y.J. Park, and H.Y. Lee, 1997. Comparison of glucose-lowering activity of the extracts from Kangwon-do mountain mulberry leaves (Moli folium) and silkworm. Kor. J. Appl. Microbiol. Biotechnol. 25(4): 391-395
  11. Kim, M.S., R.W. Choue, S.H. Chung, and S.J. Koo, 1998. Blood glucose lowering effects of mulberry leaves and silkworm extracts in mice fed with high-carbohydrate diet. The Korean Nutrition Society. 31(2): 117-125
  12. Knoll, J., 1982. Selective inhibition of B type monoamine oxidase in the brain. A drug strategy to improve the quality of life in senescence. In: Keverling-Buisman, J.A. (Ed), Strategy of Drug Research. 107-135
  13. Kwon, Y.S., H.S. Ann, T. Nabeshima, E.J. Shin, W.K. Kim, J.H. Jhoo, W.K. JHoo, M.B. Wie, Y.S. Kim, K.J. Jang, and H.C. Kim, 2004. Selegiline potentiates the effects of EGb 761 in response to ischemic brain injury. Neurochemistry International. 45: 157-170 https://doi.org/10.1016/j.neuint.2003.10.005
  14. Le, W., J. Jankovic, W. Xie, R. Kong, and S.H. Appel, 1997. (-)-deprenyl of 1-methyl-4-phenylpyridinium ion ($MPP^{+}$)-induced apotosis independent of MAO-B inhibition. Neuroscience Letters. 224: 197-200 https://doi.org/10.1016/S0304-3940(97)00170-5
  15. Levites, Y., O. Weinreb, G. Maor, M.B.H. Youdim, and S. Mandel, 2001. Green tea polyphenol (-)-epigallocatechin- 3-gallate prevents N-methyl-4-phenyl-1, 2, 3, 6-terahydropyridine-induced dopaminergic neurodegeneration. J. of Neurochemistry. 78: 1073- 1082 https://doi.org/10.1046/j.1471-4159.2001.00490.x
  16. Lowry, O.H., H.J. Rosebrough, A.L. Farr, and R.J. Randall, 1951. Protein measurment with the Folin-phenol reagent. J. Biol. Chem. 193: 265-275
  17. Pardon, M.C., C. Jounert, F. Perez-Diaz, Y. Christen, J.M. Launay, and C. Cohen-Salmon, 2000. In vivo regulation of cerebral monoamine oxidase activity in senescent controls and chronically stressed mice by long-term treatment with Ginkgo biloba extract (EGb 761). Mechanisms of Ageing and Development. 113: 157-168 https://doi.org/10.1016/S0047-6374(99)00107-4
  18. Piccinin, G.L., G. Finali, and M. Piccirilli, 1990. Neuropsychological effects of L-deprenyl in Alzheimer's type dementia. Clin. Neuropharmacol. 13(2): 147-163 https://doi.org/10.1097/00002826-199004000-00004
  19. Riederer, P., and k. Jellinger, 1983. Neurochemical insights into monoamine oxidase inhibitors with special reference to deprenyl (selegiline). Acta. Neurol. Scand Suppl. 95: 43-55
  20. Rojas, P., C. Rojas, M. Ebadi, S. Montes, A. Monroy-Noyola, and N. Serrano-Garcia, 2004. EGb761 pretreatment reduces monoamine oxidase activity in mouse corpus striatum during 1-methyl-4-phenylpyridinium neurotoxicity. Neurochemical Research. 29(7): 1417-1423 https://doi.org/10.1023/B:NERE.0000026406.64547.93
  21. Ryu, K.S., H.S. Lee, S.H. Chung, and P.D. Kang, 1997. An activity of lowering blood-glucose levels according to preparative conditions of silkworm powder. Kor. J. Seric. Sci. 39(1): 79-85
  22. Saura, J., N. Andres, C. Andrade, J. Ojuel, K. Eriksson, and N. Mahy, 1997. Biphasic and region-specific MAO-B response to aging in normal human brain. Neurobiol. Aging. 8(5): 497-507
  23. Schwarz, M.J., P.J. Houghton, S. Rose, P. Jenner, and A.D. Lees, 2003. Activities of extract and constituents of Banisteriopsis caapi relevant to parkinsonism. Pharmacology, Biochemistry, Behavior. 75: 627-633 https://doi.org/10.1016/S0091-3057(03)00129-1
  24. Soto-Otero, R., E. Mendez-Alvarez, A. Hermida- Ameijeiras, I. Sanchez-Sellero, A. Cruz-Landeira, and M.L.R. Lamas, 2001. Inhibition of brain monoamine oxidase activity by the generation of hydroxyl radicals potential implications in relation to oxidatives stress. Life Science. 69: 879-889 https://doi.org/10.1016/S0024-3205(01)01178-X
  25. Welyer, W., and J.I. Salach, 1985. Purification and properties of mitochondrial monoamine oxidase type A from human placenta. J. Biol. Chem. 260(24): 13199-13207
  26. White, H.L., P.W. Scates, and B.R. Cooper, 1996. Extract of Ginkgo biloba leaves inhibit monoamine oxidase. Life Science. 58(16): 1315-1321 https://doi.org/10.1016/0024-3205(96)00097-5
  27. Wu, W.R., and X.Z. Zhu, 1999. Involvement of monoamine oxidase inhibition in neuroprotective and neurorestorative effects of Ginkgo biloba extract against MPTPinduced nigrostriatal dopaminergic toxicity in C57 mice. Life Science. 65(1): 157-164 https://doi.org/10.1016/S0024-3205(99)00232-5