YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Inhibitory Effect on the Melanogenesis of Capsosiphon fulvescens

매생이 추출물의 멜라닌생성 억제효과

  • Mun, Yeun-Ja (Department of Herbal Resources, Professional Graduated School, Wonkwang University) ;
  • Yoo, Hyun-Ju (Department of Herbal Resources, Professional Graduated School, Wonkwang University) ;
  • Lee, Kyung-Eun (R&D Center, Hanbul Cosmetics Co.) ;
  • Kim, Jin-Hui (R&D Center, Hanbul Cosmetics Co.) ;
  • Pyo, Hyeong-Bae (R&D Center, Hanbul Cosmetics Co.) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduated School, Wonkwang University)
  • 문연자 (원광대학교 한의학전문대학원) ;
  • 유현주 (원광대학교 한의학전문대학원) ;
  • 이경은 (한불화장품 기술연구소) ;
  • 김진희 (한불화장품 기술연구소) ;
  • 표형배 (한불화장품 기술연구소) ;
  • 우원홍 (원광대학교 한의학전문대학원)
  • Published : 2005.10.01
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Abstract

The green marine algae, Capsosiphon fulvescens is one of the important economic seaweeds cultured in Korea. In this study, we investigated the effects of Capsosiphon fulvescens on melanogenesis using B16 cells. Our results showed that Capsosiphon fulvescens significantly inhibits melanin synthesis and it reduces the activity of tyrosinase, the rate-limiting melanogenic enzyme. Western Blot analysis using anti-tyrosinase antibody revealed that Capsosiphon fulvescens ($10\~40\;{\mu}g/ml$ decreased tyrosinase protein levels. Cell proliferation was dose-dependently inhibited by 10, 20 and 40 ${\mu}g/ml$ Cap­sosiphon fulvescens, without cytotoxicity and morphological change. These results suggest that the depigmenting effect of Capsosiphon fulvescens is correlated with the suppression of tyrosinase activity and protein level, which are key enzymes for melanogenesis.

Keywords

References

  1. Shimizu, H. : Chemical and biological perspectives. In Marine Natural Products Academic Press, New York, p. 1 (1978)
  2. Kobayashi, J. and Ishibashi, M. : Bioactive metabolites of symbiotic marine microorganism. Chem. Rev. 93, 1753 (1993) https://doi.org/10.1021/cr00021a005
  3. 박영현 : 해양생물독의 혈소판 응집작용에 관한 연구. 해양식품위생안정성학회지 10, 73 (1995)
  4. Kobayashi, T., Urabe, K., Winder, A., Jimenez-Cervantes C., Imokawa, G., Brewington, T., Solano, F., Garcia-Borron, J. C. and Hearing, V. J. : Tyrosinase related protein (TRP-1) functions as a DHICA oxidase activity in melanin biosynthesis. EMBO J. 13, 5818 (1994)
  5. Curto, E. V., Kwong, C., Hermersdorfer, H., Glatt, H., Santis, C., Virador, V., Hearing, V. J. and Dooley, P. : Inhibitors of mamalian melanocytes tyrosinase: In vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Biochemical Pharmacology 57, 663 (1999) https://doi.org/10.1016/S0006-2952(98)00340-2
  6. No, J. K, Soung, D. Y, Kim, Y. J., Shim, K. H., Jun, Y. S., Rhee, S. H., Yokizawa, T. and Chung, H. Y. : Inhibition of tyrosinase by green tea components. Life Science 65, 241 (1999)
  7. Prota, G. : Recent advances in the chemistry of melanogenesis in mammals. J Invest. Dermatol. 75, 31 (1990)
  8. Lee, S. H., Park, J. S., Kim, S. Y., Kim, J. J. and Chung, S. R. : Isolation of inhibitory components oa tyrosinase activity from the bark of Paeonia moutan. Yakhak Hoeji 42, 353 (1998)
  9. 박경아 외, 조직학, 고려의학, 서울, p. 405 (1999)
  10. Bloom, W. and Fawcett, D. W. : A Textbook of Histology 11th ed., W.B. Saunders Company, USA, p. 543 (1986)
  11. De Leeuw, S. M., Smit, N. P., Van Veldhoven, M., Pennings, E. M., Pavel, S., Simons, J. W. and Schothorst, A. A. : Melanin content of cultured human melanocytes and UV-induced cytoyoxicity. J Photochem. Photobiol. B. 61, 106 (2001) https://doi.org/10.1016/S1011-1344(01)00168-3
  12. Sugai, T. : Clinical effects of arbutin in patients with chlosma in Japanease. Hifu. Skin Res. 34, 522 (1992)
  13. Bliding, C. J. : A critical survey of european taxa in Ulvales, Part I, Capsosiphon, Percursaria, Blidingia, Enterromorpha. Opera Botanica 8, 1 (1963)
  14. 김도기, 서명배, 박돈희 : 녹조식물 매생이(Capsosiphon fulvescens)의 인공채묘 기술 개발. 한국생물공학회, 2001년도 춘계학술발표대회 논문집, p. 239 (2001)
  15. Hwang, E. K., Yi, Y. H., Shin, W. J. and Sohn, C. H. : Growth and maturation of a green alga, Capsosiphon fulvescens, as a new candidate for seaweed cultivation in Korea. International Seaweed Association, Proceedings of the 17th International Seaweed Symposium, p. 59 (2003
  16. 박종철, 최재수, 송상호, 최명락, 김광용, 최종원 : 해조류 추출물과 페놀성화합물의 in vitro 및 in vivo 간보호활성. 생약학회지 28, 239 (1997)
  17. Mosmann, T. : Rapid colorimetric assay for the cellular growth and survival; application to proliferation and cytotoxic assay. J. Immun. Methods 65, 55 (1983) https://doi.org/10.1016/0022-1759(83)90303-4
  18. Hosoi, J. E., Suda, T. and Kuroki, T. : Regulation of melanin synthesis of B16 mouse melanoma cells by $1{alpha}$,25 - dehydro-xyvitamin D3 and retinoic acid. Cancer Res. 45, 1474 (1985)
  19. Hunt, G., Todd, C., Cresswell, J. E. and Thody, A. J. : Alpha-melanocyte stimulating hormone and its analogue Nle4DPhe7 alpha-MSH affect morphology; tyrosinase activity and melano-genesis in cultured human melanocytes. J. Cell Sci. 107, 205 (1994)
  20. Hill, S. E., Buffey, J., Thody, A.J., Oliver, I., Bleehen, S. S. and Mac Neil, S. : Investigation of the regulation of pigmentation in alpha-melanocyte stimulating hormone responsive and unresponsive cultured B16 melanoma cells. Pigment Cell Res. 2, 161 (1989) https://doi.org/10.1111/j.1600-0749.1989.tb00181.x
  21. Martinez-Eparza, M., Jimenez-Cervantes, C., Solano, F., Lozano, J. A. and Garcia-Borron, J. C. : Mechanism of melano-genesis inhibition by tumor necrosis factor - alpha in B16/F10 mouse melanoma cells. Eur. J. Biochem. 255, 139 (1998) https://doi.org/10.1046/j.1432-1327.1998.2550139.x
  22. Chakraborty, A. K., Funasaka, Y., Komoto, M. and Ichihashi, M. : Effect of arbutin on melanogenic proteins in human melanocytes. Pigment Cell Res. 11, 206 (1998) https://doi.org/10.1111/j.1600-0749.1998.tb00731.x
  23. Hearing, V. J. and Jimenez, M. : Mammalian tyrosinase-the critical regulatory control point in melanocyte pigmentation. Int. J. Biochem. 19, 1141 (1987) https://doi.org/10.1016/0020-711X(87)90095-4
  24. Kameyama, K., Takemura, T., Hamada, Y., Sakai, C., Kondoh, S., Nishiyama, S., Urabe, K. and Hearing, V. J. : Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinase-related protein 1 (TRP1), DOPAchrome tautomerase (TRP2), and a melanogenic inhibitor. J. Invest. Dermatol. 100, 126 (1993) https://doi.org/10.1111/1523-1747.ep12462778
  25. Wakamatsu, K. and Ito, S. : Advanced chemical methods in melanin determination. Pigment Cell Res. 15, 174 (2002) https://doi.org/10.1034/j.1600-0749.2002.00056.x
  26. Naeyaert, J. M., Eller, M., Gordon, P. R., Park, H. Y. and Gilchrest, B. A. : Pigment content of cultured human melanocytes does not correlate with tyrosinase message level. Br. J. Dermatol. 125, 297 (1991) https://doi.org/10.1111/j.1365-2133.1991.tb14161.x
  27. Fuller, B. B., Drake, M. A., Spaulding, D. T. and Chaydhry, F. : Downregulation of tyrosinase activity in human melanocyte cell cultures by yohimbine. J. Invest. Dermatol. 114, 268 (2000) https://doi.org/10.1046/j.1523-1747.2000.00860.x
  28. Franchi, J., Coutadeur, M. C., Marteau, C., Mersel, M. and Kupferberg, A. : Depigmenting effects of calcium D-pantetheine-S-sulfonate on human melanocytes. Pigment Cell Res. 13, 165 (2000) https://doi.org/10.1034/j.1600-0749.2000.130308.x
  29. 이상화, 김진준, 김호정, 이종태, 강세훈 : 반하추출물이 B-16 마우스 흑색종세포의 멜라닌 생성과 타이로시네이즈 mRNA 양에 미치는 영향. 대한화장품학회지 23, 23 (1997)
  30. Ando, S., Ando, O., Suemoto, Y. and Mishima, Y. : Tyrosinase gene transcription and its control by melanogenic inhibitors. J. Invest. Dermtol. 100, 150S (1993) https://doi.org/10.1111/1523-1747.ep12465056