Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effective In Vitro Propagation from Pedicel Culture of Hippeastrum hybridum Hort. 'Dazzler'

아마릴리스 (Hippeastrum hybridum Hort. 'Dazzler') 소화경 배양에 의한 효율적 기내번식

  • Kim Myung Jun (Horticultural Science Major, Faculty of Biological Resources Science, College of Agriculture, Chonbuk National University) ;
  • Kim Young Sook (Horticultural Science Major, Faculty of Biological Resources Science, College of Agriculture, Chonbuk National University) ;
  • Kim Hyun Soon (Horticultural Science Major, Faculty of Biological Resources Science, College of Agriculture, Chonbuk National University) ;
  • Ko Jeong Ae (International Technical Cooperation Center Rural Development Administration)
  • 김명준 (전북대학교 농업생명과학대학 생물자원과학부 농업과학기술연구소) ;
  • 김영숙 (전북대학교 농업생명과학대학 생물자원과학부 농업과학기술연구소) ;
  • 김현순 (농촌진흥청 국제기술협력과) ;
  • 고정애 (전북대학교 농업생명과학대학 생물자원과학부 농업과학기술연구소)
  • Published : 2005.10.01
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Abstract

This study was conducted to establish the system of effective in vitro propagation by various explant sources culture of Bippeastrum hybridum Hort, 'Dazzler'. We tested the effects of optimal explant source, plant growth regulators on bulblet formation and plant regeneration. Callus was readily produced on the different tissues excised from floral buds whereas, bulbs and shoots were formed only on pedicel explants as compared with anthers, styles and ovaries. Pedicel is the best optimal explant for in vitro propagation. Two distinct pathways, organogenesis through callus and direct bulblet formation, could be recognized in pedicel culture. Up to the $80-100\%$ of bulblet formation and shoot organogenesis from the pedicel in fifteen days before anthesis were effectively induced by MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA. Plantlet regeneration was successfully achieved from pedicel-derived callus, via shoot bud induction or direct bulblet formation. The bulblets with blooming flower were produced within 2 years.

아마릴리스 (Hippeastrum hybridum Hort. 'Dazzler')의 효율적인 기내 급속 대량 증식체계를 확립시키고자 개화15일전 화뢰와 부착된 소화경을 재료로 하여 적합한 치상체의 선별과 캘러스 및 식물체 분화에 미치는 식물생장 조절물질의 효과를 조사하였다. 어린 화뢰내 약, 화사, 주두, 화주 및 자방은 배양 중 식물체 재분화를 위한 캘러스 및 소자구를 형성하지 못하여 배양재료로 부적합하였으나 소화경절편에서는 캘러스 및 자구가 형성되어 치상재료로 적합하였다. 배지내 첨가된 식물생장조절물질에 따라 치상체에서 직접 자구를 형성하거나 캘러스를 통해 식물체로 분화되는 2가지 경로로 형성되었는데 개화 15일전 소화경 절편을 0.5 mg/L LAA와 1.0 mg/L BA가 혼용처리된 MS 배지에 배양하여 $100\%$ 소자구형성 및 신초가 분화되어 효과적이었으며 소화경에서 직접 형성된 자구 또는 캘러스를 통해 분화된 신초는 성공적으로 식물체로 재분화 되었다.

Keywords

References

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