Analysis of Extraction Characteristics of Phytoestrogen Components from Punica granatum L.

석류 phytoestrogen 성분의 추출특성 분석

  • Kim, Seong-Ho (Department of Herb & Food Science, Kyungpook College of Science) ;
  • Kim, In-Ho (Department of Bio-Health Industrial Institute, Kyungpook College of Science) ;
  • Kang, Bok-Hee (Department of Bio-Health Industrial Institute, Kyungpook College of Science) ;
  • Cha, Tae-Yang (Department of Bio-Health Industrial Institute, Kyungpook College of Science) ;
  • Lee, Jin-Hyung (Department of Microbiology, Kyungpook National University) ;
  • Kim, Jong-Myeong (Department of Microbiology, Kyungpook National University) ;
  • Rim, Soon-Ok (Department of Microbiology, Kyungpook National University) ;
  • Song, Kyung-Sik (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Song, Bang-Ho (Department of Biology Education, Kyungpook National University) ;
  • Kim, Jong-Guk (Department of Microbiology, Kyungpook National University) ;
  • Lee, Jin-Man (Department of Herb & Food Science, Kyungpook College of Science)
  • 김성호 (경북과학대학 약용식품과/바이오건강산업연구소) ;
  • 김인호 (경북과학대학/바이오건강산업연구소) ;
  • 강복희 (경북과학대학/바이오건강산업연구소) ;
  • 차태양 (경북과학대학/바이오건강산업연구소) ;
  • 이진형 (경북대학교 미생물학과) ;
  • 김종명 (경북대학교 미생물학과) ;
  • 임순옥 (경북대학교 미생물학과) ;
  • 송경식 (경북대학교 농업생명과학대학) ;
  • 송방호 (경북대학교 생물교육과) ;
  • 김종국 (경북대학교 미생물학과) ;
  • 이진만 (경북과학대학 약용식품과/바이오건강산업연구소)
  • Published : 2005.12.31

Abstract

The optimization of extraction conditions of phytoestrogen from pomegranate by hot water was conducted by analyzing the extraction characteristics. The purpose of this study was effective utilization of bioactive components of pomegranate, and the analyzing was performed with response surface methodology (RSM). This study established 10 sections based on the central composite design with the independent variables of extraction temperature (60, 70, 80, 90, $100^{\circ}C$) and extraction time (1, 2, 3, 4, 5 hr) to predict the optimal conditions for extraction of the effective components. The dependent variables were measured for extracted materials, those were, the major components such as chlorogenic acid, kaempferol, $17-{\alpha}-estradiol\;and\;17-{\beta}-estradiol$ content, and regression analysis was performed by SAS program, and optimal conditions for each characteristics were predicted, and the characteristics of extraction were analyzed by response surface methodology. It was found that chlorogenic acid, kaempferol, and $17-{\alpha}-estradiol$ content were greatly affected by extraction temperature. However, $17-{\beta}-estradiol$ content was affected significantly by extraction time. Regression formulas for each variable were elicited from this study, and the chlorogenic acid, kaempferol, $17-{\alpha}-estradiol\;and\;17-{\beta}-estradiol$ content depending on response surface methodology factor were superimposed. It was shown that optimal temperature and extraction time were $98{\sim}100^{\circ}C\;and\;3{\sim}5$ hrs, respectively.

건강기능성식품의 원료로 이용되고 있는 석류의 주요성분에 대한 효율적인 이용을 위하여 반응표면분석에 의하여 각 성분의 열수 추출조건을 분석하여 최적 추출조건을 확립하였다. 주요성분의 최적 추출조건 예측을 위하여 추출온도$(60{\sim}100^{\circ}C)$, 추출시간$(1{\sim}5hr)$을 독립변수로 하여 중심합성계획에 의해 10구의 추출조건을 설정하였다. 각 추출물의 주요성분 함량 즉, chlorogenic acid, kaempferol, $17-{\alpha}-estradiol$$17-{\beta}-estradiol$ 함량을 측정하여 SAS program에 의해 회귀분석을 하여 각각의 특성을 분석하였다. 그 결과 chlorogenic acid, kaempferol 및 $17-{\alpha}-estradiol$ 함량은 주로 추출온도에 영향을 받는 것으로 나타났으며, $17-{\beta}-estradiol$ 함량은 추출시간에 영향을 받는 것으로 나타났다. 각 변수에 대한 회귀식을 도출하여 공정인자별 석류 추출물의 chlorogenic acid, kaempferol, $17-{\alpha}-estradiol$$17-{\beta}-estradiol$ 함량에 대한 최적 추출조건을 superimpose한 결과 추출온도 $98{\sim}100^{\circ}C$, 추출시간 $3{\sim}4hr$로 각각 예측되었다.

Keywords

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