Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Screening of Biological Activities of the Extracts from Basil (Ocimum basilicum L.)

Basil(Ocimum basilicum L.) 추출물의 생리활성 탐색

  • Kim, Jeung-Hoan (Department of Food Engineering Sangju National University) ;
  • Yoon, So-Jung (Department of Food Engineering Sangju National University) ;
  • Lee, Kyoung-Hwan (Department of Food Engineering Sangju National University) ;
  • Kwon, Hyo-Jung (Department of Food Engineering Sangju National University) ;
  • Chun, Sung-Sook (Department of Food Science & Technology Yeungnam University) ;
  • Kim, Tae-Wan (Institute of Agricultural Science & Technology Kyungpook National University) ;
  • Cho, Young-Je (Department of Food Engineering Sangju National University)
  • 김정환 (상주대학교 식품공학과) ;
  • 윤소정 (상주대학교 식품공학과) ;
  • 이경환 (상주대학교 식품공학과) ;
  • 권효정 (상주대학교 식품공학과) ;
  • 천성숙 (영남대학교 식품가공학과) ;
  • 김태완 (경북대학교 농업과학 기술연구소) ;
  • 조영제 (상주대학교 식품공학과)
  • Published : 2005.06.30
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Abstract

Physiological functionalities of water and ethanol extracts from Basil were determined. The concentration of total phenolic compounds of the water and ethanol extracts were $286.0\;{\mu}g/ml$, $250.0\;{\mu}g/ml$. Antioxidant activities of Basil extracts were determined using 2,2'-azinobis (3-ethyl benzothiazoline-6-sulfonic acid) radical cations (ABTS), 2,2-diphenyl-1-picryl hydrazyl radicals (DPPH), antioxidant protection factor and thiobarbituric acid reactive substances. The total antioxidant activities of Basil extracts using ABTS were 96.8% in the water extracts and 94.7% in the ethanol extract, DPPH were 87.0%, 93.9%, PF were 0.69, 1.16 and TBARS were $0.2{\times}10^{-3}\;{\mu}M,\;0.6{\times}10^{-3}\;{\mu}M$. Angiotensin converting enzyme inhibitory activity and xanthine oxidase inhibitory activity of Basil were higher in ethanol extracts (99.7%, 100.0%) than those of water extracts (39.9%, 54.7%). Phenolic profiles in Basil extracts were analyzed using HPLC. The result was that among the 6 phenolics, rosemarinic acid was the highest in ethanol extracts.

Basil을 물과 에탄올로 추출하여 생리 기능성을 알아 보았다. 추출물의 총 페놀 함량은 열수 추출물이 $286.0\;{\mu}g/ml$, 알코올 추출물이 $250.0\;{\mu}g/ml$로 나타났다. 추출물의 항산화 효과는 DPPH가 열수 추출물과 알코올 추출물이 각각 87.0%, 93.9%로 나타났고, antioxidant protection factor는 각각 0.7, 1.2으로 낮게 나타났다. ABTS는 열수 추출물이 96.8%, 알코올 추출물이 94.7%로 높게 나타났으며 TBARS는 $0.2{\times}10^{-3}\;{\mu}M,\;0.6{\times}10^{-3}\;{\mu}M$로 대조구 $2.9{\times}10^{-3}\;{\mu}M$에 비해 malonaldehyde와 thiobarbituric acid가 반응하여 생성되는 복합체 TBARS값이 낮아 추출물이 지방 산화에 영향을 주는 것을 알 수 있었다. ACE 저해 효과와 XOase 저해 효과는 알코올 추출물에서 각각 96.6%, 100.0%로 열수 추출물 각각 39.9%, 54.7%보다 높게 나타났다. HPLC로 분석한 결과 알콜 추출물에서 rosemarinic acid가 가장 많은 것으로 나타 났다. 이 결과로 Basil은 인공 합성 항산화제가 가지는 단점을 보완할 수 있는 천연 항산화제 및 통풍 저해와 항 고혈압 기능을 겸비한 기능성 소재로의 개발이 가능할 것으로 생각된다.

Keywords

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