Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antimicrobial Activity against Helicobacter pylori and Antioxidant Activity of Yerusalem sage (Phlomis frutcosa L.)

예루살렘세이지(Phlomis frutcosa L.) 추출물의 Helicobacter pylori에 대한 항균활성과 항산화활성

  • Kim, Jeung-Hoan (Department of Food Engineering Sangju National University) ;
  • Yoon, So-Jeung (Department of Food Engineering Sangju National University) ;
  • Cho, Young-Je (Department of Food Engineering Sangju National University)
  • 김정환 (상주대학교 식품공학과) ;
  • 윤소정 (상주대학교 식품공학과) ;
  • 조영제 (상주대학교 식품공학과)
  • Published : 2005.06.30
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Abstract

The concentration of total phenolic compounds of the water and ethanol extracts of Yerusalem sage were 9.4 mg/g and 11.1 mg/g respectively. The total antioxidant activities of water and ethanol extracts of Yerusalem sage were 96.8% and 91.4% in ABTS assay and 97.6% and 87.2% in DPPH assay, PF of the water and ethanolic extracts were 1.1 and 1.6, respectively. TBARS were $0.9{\times}10^{-3}\;{\mu}M$ and $0.3{\times}10^{-3}\;{\mu}M$, respectively. Antimicrobial effect against H. pylori of the ethanol extract was $9{\sim}15\;mm$ of clear zone diameter at $50\;{\mu}g/ml{\sim}200\;{\mu}g/ml$ of phenolic compound. Phenolic profiles in Yerusalem sage chlorogenic aicd was the highest both in water and ethanol extracts in HPLC analyses.

Yerusalem sage를 물과 에탄올로 추출하여 생리 기능성을 알아보았다. 추출물의 총 페놀 함량은 물 추출물이 9.4 mg/g, 알코올 추출물이 11.1 mg/g로 나타났고 추출물의 항산화 효과는 DPPH가 물 추출물과 알코올 추출물 각각 97.6%, 87.2%로 나타났고, antioxidant protection factor는 각각 1.1, 1.6으로 알콜 추출물이 물 추출물보다 높게 나타났다. ABTS는 물 추출물과 알코올 추출물에서 각각 96.8%, 91.4%로 높게 나타났으며 TBARS 값은 $0.9{\times}10^{-3}\;{\mu}M,\;0.3{\times}10^{-3}\;{\mu}M$로 대조구 $1.1{\times}10^{-3}\;{\mu}M$에 비해 malonaldehyde와 thiobarbituric acid가 반응하여 생성되는 복합체 TBARS값이 낮아 추출물이 지방 산화에 영향을 주는 것을 알 수 있었다. Helicobacter pylori균의 항균활성 측정을 위하여 알콜 추출물의 phenolic compound 함량을 $50\;{\mu}g$에서 $200\;{\mu}g$까지 $50\;{\mu}g$씩 disc paper에 증가시켜 첨가한 결과 저해환의 직경이 첨가량이 증가할수록 9 mm, 10 mm, 12 mm, 15 mm로 크게 나타났다. HPLC로 항산화 및 항균력을 가진 페놀산의 존재유무를 분석한 결과 물과 알코올 추출물에서 chlorogenic acid함량이 가장 많은 것으로 나타났다. 이 결과로 Yerusalem sage는 인공 합성 항산화제가 가지는 단점을 보완할 수 있는 천연 항산화제 및 위 십이지장 계통의 질환을 예방하는 기능을 겸비한 기능성 물질로 개발이 기대되었다.

Keywords

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