Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Synergistic Effect of Physico-chemical Treatment and Bacteriocin Produced by Enterococcus faecium MJ-14

Enterococcus faecium MJ-14가 생산하는 박테리오신과 물리화학적 처리의 상승효과

  • Lim Sung-Mee (Department of Hotel Cuilinary Arts, Tongmyong College)
  • Published : 2005.12.01
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Abstract

When L. monocytogenes ($10^{5}CFU/mL$) at exponential phase cells were heated for 5 min at $65^{\circ}C$ in the presence of the bacteriocin (30 BU/mL) produced by E. faecium MJ-14, the number of viable cells was markedly reduced at p < 0.05. The bactericidal effect of bacteriocin showed synergism with combination ot organic acids (citric acid or acetic acid) or chemical preservatives (sodium benzoate, sodium lactate, sodium nitrate or potassium nitrate). For example, the number of viable cells was reduced by 4.8 log units under combination of the bacteriocin (30 BU/mL) and sodium nitrate ($100{\mu}g/mL$), while it was reduced by 1.1 log unit only under single treatment of the bacteriocin after 12 k at $37^{\circ}C$. The addition of the bacteriocin (300 BU/mL) into skim milk inoculated with L. monocytogenes ($10^{5}$CFU/mL) reduced the cells by 1.5 log unit, in case of the cell suspension stored at $4^{\circ}C$ for 24 hr. Moreover, L. monocytogenes was reduced by 2 log unit when stored at $-20^{\circ}C$ for 7 days in gound pork added with 300 BU/mL of 린e bacteriocin.

E.faecium MJ-14가 생산하는 박테리오신 30 BU/ML 처리에 의해 L. monocytogepes 균수는 0.6 log unit 감소 되었으나, 박테리오신 (30 BU/mL $65^{\circ}C$, 5분간 가열처리와 병용한 경우에는 약 2.7 log unit가 감소하였다. 박테리오신 30 BU/mL와 itric acid혹은 acetic acid $0.5\%$를 병용 처리한 경우에는 24시간 만에 초기균수가 각각 약 2 log unit와 3 log unit감소하였다. 박테리오신 30 BU/mL와 NaCl $8\%$를 병용 처리했을 때에는 박테리오신 단독 처리구보다 약 1 log unit더 감소되었다. 또한, 박테리오신 30 BU/mL단독 처리했을 때에 비해 sodium benzoate $500{\mu}g/mL$혹은 sodium lactate $1000{\mu}g/mL$와 각각 병용 처리하면 2 log unit가 더 감소되었으며, sodium nitrate $100{\mu}g/mL$와 potassium nitrate $100{\mu}g/mL$와의 병용 처리한 경우 박테리오신 단독 처리구에 비해 각각 3.5 log unit와 2 log unit이상 더 감소하였다. 한편, 탈지유에 E. faecium MJ-14의 박테리오신 300 BU/mL을 첨가한 경우 $4^{\circ}C$에서 24시간 만에 L. monocytogenes의 균수는 대조구에 비해 각각 약 1.5 log unit감소되었으며,돈육에 첨가한 경우 $-20^{\circ}C$에서 7일간 만에 대조구보다 약 2 log unit의 감소효과가 나타났다.

Keywords

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