Long-tenn Evaluation of Extruded Pellet Diets Compared to Raw Fish Moist Pellet Diet for Growing Flounder, Paralichthys olivaceus

넙치 미성어 건조 배합사료 및 습사료의 장기사육 평가

  • 김장웅 (국립수산과학원 양식사료연구센터) ;
  • 강용진 (국립수산과학원 양식사료연구센터) ;
  • 김경민 (국립수산과학원 양식사료연구센터) ;
  • 이해영 (국립수산과학원 양식사료연구센터) ;
  • 김경덕 (국립수산과학원 양식사료연구센터) ;
  • 배승철 (부경대학교 양식학과)
  • Published : 2005.11.01

Abstract

This experiment was conducted to compare the effects of extruded pellets and raw fish-based pellet on olive flounder Paralichthys. olivaceus. Six diets were prepared for this study: two formulated extruded pellets (FEP1 & FEP2), three commercially available extruded pellets (CEP1, CEP2 & CEP3) and moist pellet (MP). Weight gain offish fed FEP1 and CEP3 were significantly higher (P<0.05) than those of fish fed FEP2, CEP1, CEP2 and MP, while that of fish fed MP was not significantly different (f<0.05) from those of fish fed the FEP2, CEP1 and CEP2. Feed efficiency of fish fed CEP2 was significantly lower (P<0.05) than those of fish fed FEP1, FEP2, CEP1, CEP3 and MP. There was no significant difference in protein efficiency ratio and hepatosomatic index between fish fed FEP1 and CEP3, and among fish fed FEP2, CEP1 and CEP2. There was no significant difference in condition factor among fish fed the FEP1 and CEP3, and between fish fed FEP2, CTP1 and MP. However, fish fed MP had a lower survival rate than fish fed the other five EP These results suggest that diet FEPl could be developed to replace MP for the owing stage of flounder without adverse effects on growth performance.

이 연구는 배합사료(상품사료)및 생사료의 영양학적 효과를 비교${\cdot}$평가를 통해 경제적이고 환경친화적인 넙치용 실용 배합사료를 개발하고자 장기사육시험을 수행하였다. 실험어는 평균 192 g인 넙치 미성어를 사용하였으며, 어체중의 $1{\~}2.5\%$ (건물기준)로 1일 2회(오전 9시, 오후 4시)급이 하였으며, 주 사육실험기간은 12개월간 실시하였다. 실험사료는 충 6가지 사료구로 자체 제작한 실험배합사료 2종(FEP1과 2), 상업용 배합사료 3종(CEP1, 2와 3) 및 습사료(MP)로 공급하였으며, 자체 제작한 넙치 미성어 배합사료(FEP1과 2)는 단백질 함량 $50{\~}51\%$, 지질 $10{\~}12\%$로 설계되었다. 증체율은 FEP1 및 CEP3 사료구가 다른 사료구들과 비교하여 유의적으로 높은 증체율을 보였으며, FEP2, CEP1, CEP2, MP 사료구에서 유의적으로 낮은 증체율을 보였다(P<0.05). 사료효율 및 일간성장률에 있어서는 FEP1 및 CEP3 사료구가 다른 사료구들과 비교하여 유의적으로 높게 나타났다. 단백질 전환효율은 MP 사료구가 FEPl 및 CEP3 사료구가 다른 사료구들과 비교하여 유의적으로 높게 나타났다(P<0.05). 생존율에 있어서 배합사료구가 MP사료구보다 유의적으로 높게 나타났다(P>0.05). 혈청 성분분석 결과는 GOT, GPT 및 Total cholesterol에 있어서는 전체적으로 배합사료구보다 HP 사료구에서 높게 나타났다. 전어체 성분분석에 있어서 조단백질함량은 FEP1 및 CEP3에서 다른사료구보다 높은 경향을 보였다. 따라서, 넙치 미성어의 FEP1은 상업사료 및 습사료보다 충분히 가능성이 있으며, 양식장 현장실험을 거쳐서 산업화 보급을 할 수 있을 것으로 생각된다.

Keywords

References

  1. Alam, M. S., S. I. Teshima, S. Kosiho and M. Ishikawa, 2002 Arginine requirement of juvenile Japanese flounder, Paralichthys olivaceus estimated by growth and biochemical parameters. Aquaculture, 205, 127-140 https://doi.org/10.1016/S0044-8486(01)00670-6
  2. AOAC (Association of Official Analytical Chemists), 1984, Official methods of analysis, 16th edition. AOAC International Arlington, Virginia
  3. Bai, S. C, Y. T. Cha and X. J. Wang, 2002. A preliminary study on the dietary protein requirement of larval Japanese flounder. North American Journal of Aquaculture, 63, 92-98 https://doi.org/10.1577/1548-8454(2001)063<0092:APSOTD>2.0.CO;2
  4. Cho, S. H., S. M. Lee and J. H. Lee, 2005. Effects of the extruded pellets and raw fish-based moist pellet on growth and body composition of flounder, Parlichthys olivaceus for 10 months. J. Aquacul., 18, 60-65
  5. Forster, I. and H. Y. Ogata, 1998. Lysine requirement of juvenile Japanese flounder, Paralichthys olivaceus and juvenile red sea bream, Pagrus major. Aquaculture, 161, 131-142 https://doi.org/10.1016/S0044-8486(97)00263-9
  6. Garrido, L. G., R. M. Chapuli and A. V. Andres, 1990. Serum cholesterol and triglyceride levels in Scyliorhinus canicula (L.) during sexual maturation. J. Fish Biol., 36, 499-509 https://doi.org/10.1111/j.1095-8649.1990.tb03552.x
  7. Gordon, R. B., 1968. Distribution of transaminase (Aminotransferases) in the tissues of the pacific salmon, with emphasis on the properties and diagnostic use of glutamic oxaloacetic transaminase. J. fish. Res. Bd. Can., 25, 1247-1268 https://doi.org/10.1139/f68-108
  8. Jauncey, K., 1982. The effects of varying dietary protein level on the growth, food conversion, protein utilization and body composition of juvenile tilapias (Sarotherodon mossambicus). Aquaculture, 27, 43-54 https://doi.org/10.1016/0044-8486(82)90108-9
  9. Kikuchi, K., T. Furuta and H. Honda, 1997. Use of Meat and Bone Meal as a protein source in the diet of juvenile Japanese flounder. Fish. Sci., 63, 29-32 https://doi.org/10.2331/fishsci.63.29
  10. Kim, J. D., S. H. Shin, K. J. Cho and Y. H. Lee, 2002c. Effect of daily and alternate day feeding regimens on growth and food utilization by juvenile flounder. J. Aquacul., 15, 15-21
  11. Kim, K. D. and S. M. Lee, 2004. Requirement of dietary n-3 highly unsaturated fatty acids for juvenile flounder Paralichthys olivaceus. Aquaculture, 229, 315-323 https://doi.org/10.1016/S0044-8486(03)00356-9
  12. Kim, K. D., S. M. Lee, H. G Park, S. C. Bai. and Y. H. Lee, 2002b, Essentiality fo dietary n-3 highly unsaturated fatty acids in juvenile Japanese flounder, Paralichthys olivaceus. J. World Aquacult. Soc., 33, 432-440 https://doi.org/10.1111/j.1749-7345.2002.tb00022.x
  13. Kim, K. W, X. J. Wang and S. C. Bai, 2002a. Reevaluation of the Dietary Protein Requirement of Japanese Flounder Paralichthys olivaceus. Aquaculture research, 33, 673-679 https://doi.org/10.1046/j.1365-2109.2002.00704.x
  14. Kim, K. W, X. J. Wang, S. M. Choi, G J. Park and S. C. Bai, 2004, Evaluation of optimum dietary protein-to-energy ratio in juvenile olive flounder, Paralichthys olivaceus. Aquacult. Res., 35, 250-255 https://doi.org/10.1111/j.1365-2109.2004.01003.x
  15. Lee, C. H., 1993, The development of ceroidosis in cultured flounder, paralichthys olivaceus. J. Fish Pathol., 6, 143-161
  16. Lee, S. M., C. S. Park and I. C. Bang, 2002. Dietary protein requirement of young Japanese flounder Paralichthys olivaceus fed isocaloic diets. Fish. Sci., 68, 158-164 https://doi.org/10.1046/j.1444-2906.2002.00402.x
  17. Lee, S. M., S. H. Cho and K. D. Kim, 2000. Effects of dietary protein and energy levels on growth and body composition of juvenile flounder Paralichthys olivaceus. J. Aquacult. Soc., 31, 306-315
  18. Lee, S. M., K. D. Kim and S. P. Lall, 2003. Utilization of glucose, maltose, dextrin and cellulose by juvenile flounder. Aquaculture, 221, 427-138 https://doi.org/10.1016/S0044-8486(03)00061-9
  19. Mosconi-Bac, N., 1987, Hepatic desturbances induced by an artificial feed in the sea bass (Dicentrarchus labrax) during the first year of life. Aquaculture, 67, 93-99 https://doi.org/10.1016/0044-8486(87)90012-3
  20. Ministry of Maritime Affairs and fisheries (MOMAF), 2004. 해양수산부
  21. Murai, T., T. Akiyama and T. Takeuchi, 1985, Effect of dietary protein and lipid levels on performance and carcass composition of fingerling carp. Bull. Jap. Soc. Sci. Fish, 51, 605-608 https://doi.org/10.2331/suisan.51.605
  22. Nandeesha, M. C, S. S. De Silva and D. S. Murthy, 1995, Use of mixed feeding schedules in fish culture: performance of common carp, Cyprinus carpio L., on plant and animal protein based diets. Aquacutl. Res., 26, 161-166 https://doi.org/10.1111/j.1365-2109.1995.tb00898.x
  23. National Research Council (NRC). Nutrient Requirements of Fish. National Acad. Press, Washington, DC, 1993
  24. New, M. B., 1999, Global Aaquaculture: Current trends and challenges for the 21st Century. Aquaculture, 30, 8-13
  25. Peres, H. and A. Oliva-Teles, 1999, Effect of dietary lipid level on growth performance and feed utilization by European sea bass juvenile (Dicentrarchus labrax). Aquaculture, 179, 325-334 https://doi.org/10.1016/S0044-8486(99)00168-4
  26. Rodriguez-Serna, M., M. A. Olvera-Novoa and C. Carmona-Osalde, 1996. Nutritional value of animal by-product meal in practical diets for Nile tilapia Oreochromis niloticus (L.) fry. Aquacult. Res., 27, 67-73 https://doi.org/10.1111/j.1365-2109.1996.tb00967.x
  27. Sato, T., 1998, Development of formulated feeds for juvenile Japanese flounder. Ph. D thesis. Tokyo University of Fisheries, Tokyo, Japan
  28. Sato, T. and K. Kikuchi, 1997. Meat meal as a protein source in the diet of juvenile Japanese flounder, Fish. Sci, 63, 877-880 https://doi.org/10.2331/suisan.63.877
  29. Scott, M. L., M. G Nesheim and R. J. Young, 1976, Feedstuffs for poultry. In: Nutrition of the Chicken, 2nd edn. M. L. Scott and Associates, Ithaca, NY, Chapter 8, 428-466
  30. Serrano, J. A., G R. Nematipour and D. M. Gatlin, 1992, Dietary protein requirement of the red drum (Sciaenops ocellatus) and relative use of dietary carbohydrate and lipid. Aquaculture, 101, 283-291 https://doi.org/10.1016/0044-8486(92)90031-F
  31. Wang, X. J., K. W. Kim and S. C. Bai, 2002, Effects of different dietary levels of L-ascorbyl-2-polyphosphate on growth and tissue vitamin C concentrations in juvenile olive flounder, Paralichthys olivaceus, Aquacul. Res., 33, 261-267 https://doi.org/10.1046/j.1355-557x.2002.00669.x