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Occurrence and Symptoms of Tomato Spotted Wilt Virus on Vegetables in Korea (I)

채소류의 토마토 반점 위조 바이러스 발생과 병징 (I)

  • Cho Jeom-Deog (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim Jeong-Soo (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim Jin-Young (Gyunggi Province Agricultural Research and Extension Service) ;
  • Kim Jae-Hyun (KT&G Central Research Institute) ;
  • Lee Sin-Ho (National Horticultural Research Institute, Rural Development Administration) ;
  • Choi Gug-Seoun (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim Hyun-Ran (National Horticultural Research Institute, Rural Development Administration) ;
  • Chung Bong-Nam (National Horticultural Research Institute, Rural Development Administration)
  • Published : 2005.12.01

Abstract

Tomato spotted wilt virus (TSWV) occurred abruptly with a high incidence rate in 14 vegetable crops in Anyang area, Gyunggido in 2004. TSWV was identified by the symptoms on the indicator plants, immunocaptured reverse transcription-polymerase chain reaction (IC/RT-PCR), virion captured (VC)RT-PCR and RT-PCR using total RNA from the infected plants. Vegetable crops infected with TSWV included table tomato, cherry tomato, red pepper, lettuce, chicory, red leaf chicory, red mustard, dragon tongue, treviso, potato, perilla, sesame, pumpkin, and ssamchoo (hybrid of oriental cabbage and cabbage). The incidence of TSWV in fields ranged from 30 to $100\%$. TSWV usually produced necrosis, wilt and/or severe mosaic with typical single or double ring spots on the leaves. TSWV could be detected in Frankliniella occidentalis collected from the crops in the fields with $90\%$ rate by IC/RT-PCR.

토마토 반점위조 바이러스(Tomato spotted wilt virus; TSWV)가 2004년 경기도 안양지역에서 토마토, 고추 등을 포함한 14개 채소 작물에서 발생하였다. TSWV검정은 지표식물 검정, IC/RT-PCR, VC/RT-PCR 및 Total RNA를 이용한 RT-PCR방법을 이용하였으며, TSWV가 발생한 작물의 종류는 토마토, 방울 토마토, 고추, 시금치, 치커리, 적치커리, 적겨자, 용설채, 트레비소, 감자, 들깨, 참깨, 호박, 쌈추 이었다. 포장에서의 발생율은 토마토, 고추 등 주요 작물에서 $30\%$에서 $100\%$ 발생하였으며,병징은 대부분 전형적인 원형반점이었으며, 괴저, 위조 및 심한 모자이크 병징으로 진전되었다. TSWV가 발생한 포장에서 채집한 꽃노랑 총채벌레(Frankliniella occidentalis)를 IC/RT-PCR 검정한 결과 감염율이 $90\%$이었다.

Keywords

References

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