Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of Oxolinic Acid in Fish Products Using HPLC

HPLC를 이용한 어류 중의 Oxolinic Acid 분석

  • LEE Hee Jung (National Fisheries Research and Development Institute) ;
  • LEE Tae Seek (National Fisheries Research and Development Institute) ;
  • SON Kwang Tae (National Fisheries Research and Development Institute) ;
  • KIM Poong Ho (National Fisheries Research and Development Institute) ;
  • JO Mi Ra (National Fisheries Research and Development Institute) ;
  • PARK Mi Jung (National Fishes Products Quality Inspection Service) ;
  • Yi Young Ho (The National Assembly)
  • Published : 2005.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

A high-performance liquid chromatography assay method for oxolinic acid in fish products was developed, evaluated and validated through the monitoring of oxolinic acid based on farming and distribution. The recovery rate of the developed method was $102.3-106.7\%$ as compared to conventional methods. The stock solution was stable for 3 weeks under refrigerated condition at $4^{\circ}C$ The performance limit was evaluated as 0.01ppm of oxolinic acid in fish muscle. 478 fish samples such as olive flounder, genuine porgy, common sea bass and black rock fish collected from fish farms in the coastal area from September 2001 to October 2004 were analyzed to evaluate overall efficiency of the modified method and to monitor the actual condition of oxolinic acid usage in fish farm. According to the monitoring results, the modified method was suitable for analysis of oxolinic acid in fish muscle and oxolinic acid might be used in a small portion of fish farms. The suggested analysis method of oxolinic acid was registered in the Korean Official Methods of Food Analysis and is being utilized for fishery products by the Korea Food and Drug Adminstration and the National Fisheries Products Quality Inspection Service.

Keywords

References

  1. Chun, S.K. 1992. Guidance for use of aquaculture medicine. In: Disease of marine farmed fish. Susan Times, Seoul, 126-131
  2. Hamamoto, K. 1986. Rapid high-performance liquid chromatographic method for the determination of oxolinic acid in chicken plasma. J. Chromatogr. B, 381, 453-456 https://doi.org/10.1016/S0378-4347(00)83614-2
  3. Horii, S., C. Yasuoka and M. Matsumoto. 1987. Highperformance liquid chromatographic method for the simultaneous determination of oxolinic, nalidixic and piromidic acids in cultured fish. J. Chromatogr. A, 388, 459-466 https://doi.org/10.1016/S0021-9673(01)94507-8
  4. Hustvedt, S.O., R. Salte and V. Vassik. 1991. Absorption, distribution and elimination of oxolinic acid in Atlantic salmon after various routes of administration. Aquaculture, 95, 193-199 https://doi.org/10.1016/0044-8486(91)90086-M
  5. Ikai, Y., H. aka, N, Kawamura, M. Yamada, K. Harada, M. Suzuki and H. Nakazawa. 1989. Improvement of chemical analysis of antibiotics: XVI. Simple and rapid determination of residual pyridonecarboxylic acid antibacterials in fish using a prepacked amino cartridge J. Chromatogr. A, 477, 397-406 https://doi.org/10.1016/S0021-9673(01)89648-5
  6. Inglis, V. and R.H. Richards. 1991. The in vitro susceptibility of Aeromonas salmonicida and other fish pathogenic bacteria to 29 antimicrobial agent. J. Fish Dis., 14, 641-650 https://doi.org/10.1111/j.1365-2761.1991.tb00622.x
  7. Jacobsen, M.D. 1989. Withdrawal times of freshwater rainbow trout after treatment with oxolinic acid, oxytetracycline and trimethoprim. J. Fish Dis. 12, 29-36 https://doi.org/10.1111/j.1365-2761.1989.tb01287.x
  8. Jeong, H.D. and S.K. Chun. 1992. The utilization of antibiotics and the treatment of bacterial diseases in fish. J. Fish Pathol., 5, 37-48
  9. Kasuga, Y., A. Sugitani and F. Yamada. 1982. Simultaneous determination of oxolinic, nalidixic and piromidic acids in fishes by high performance liquid chromatography. J. Food Hyg. Soc. Jpn., 23, 344-347 https://doi.org/10.3358/shokueishi.23.344
  10. KAHPA (Korea Animal Health Products Association). 2001. The Guidebook of Veterinary Antibiotics, Kyungsung Munhwasa, GyeongGi-Do, 645-653
  11. KFDA (Korea Food and Drug Administration). 2000. Korea Food Code (A supplementary), Chapter 7. General method for examination, Munyoungsa, Seoul, 363-365
  12. Kim, K.H., M.R. Song, S.N. Choe, M.S. Choe and K.H. Park. 1998. Oxolinic acid residue in the cultured eel tissues and its change to heating process. J. Fd. Hyg. Safety, 13, 14-19
  13. KQS (Kobe Quarantine Station). 1996. Analysis of synthetic antimicrobials in livestock and seafood products imported. - Rapid determination of residual synthetic antibacterials in fish and meat by HPLC with linear gradient elution. Bull. Kobe Quarant. Station, 1-7
  14. Larocque, L., M. Schnurr, S. Sved and A. Weninger. 1991. Determination of oxolinic acid residues in salmon muscle tissue by liquid chromatography with fluorescence detection. J. Assoc. Off. Anal. Chern., 74, 608-611
  15. Nose, N., Y. Hashino, Y. Kikuchi, M. Horie, K. Saitoh, T. Kawachi and H. Nakazawa. 1987. Simultaneous liquid chromatographic determination of residual synthetic antibacterials in cultured fish. J. Assoc. Off. Anal. Chern., 70, 714-717
  16. Pouliquen, H. and F. Armand. 2000. Determination of oxolinic acid in faeces and turbot (Scophthalmus maximus) by high-performance liquid chromatography using fluorescence detection. J. Chromatogr. B, 749, 127-133 https://doi.org/10.1016/S0378-4347(00)00394-7
  17. Ueno, R., M. Okumura, Y. Horiguchi and S.S. Kubota. 1988. Levels of oxolinic acid in cultured rainbow trout and amago salmon after oral administration. Nippon Suisan Gakkaishi, 54, 485-489 https://doi.org/10.2331/suisan.54.485

Cited by

  1. Determination of oxolinic acid residues in the muscle tissue of olive flounder (Paralichthysolivaceus) by a lateral flow immunoassay vol.27, pp.3, 2016, https://doi.org/10.1080/09540105.2015.1104656
  2. Alcohol Metabolizing Activity of Fermented Sea Tangle Juice vol.43, pp.1, 2010, https://doi.org/10.5657/kfas.2010.43.1.001
  3. Residues of Antibiotics in Wild and Cultured Fishes Collected from Coast of Korea vol.43, pp.1, 2010, https://doi.org/10.5657/kfas.2010.43.1.012