KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Characteristics of Biochemical Markers and Whole-Wheat Flours Using Small-Scaled Sampling Methods in Korean Wheats

  • Park Chul Soo (Division of Winter Cereal and Upland Crops, Homan Agricultural Research Institute, NICS) ;
  • Kim Yang-Kil (Division of Winter Cereal and Upland Crops, Homan Agricultural Research Institute, NICS) ;
  • Han Ouk-Kyu (Division of Winter Cereal and Upland Crops, Homan Agricultural Research Institute, NICS) ;
  • Lee Mi Ja (Division of Winter Cereal and Upland Crops, Homan Agricultural Research Institute, NICS) ;
  • Park Jong-Chul (Division of Winter Cereal and Upland Crops, Homan Agricultural Research Institute, NICS) ;
  • Seo Jae-Hwan (Division of Winter Cereal and Upland Crops, Homan Agricultural Research Institute, NICS) ;
  • Hwang Jong-Jin (Division of Winter Cereal and Upland Crops, Homan Agricultural Research Institute, NICS) ;
  • Kim Jung-Gon (Division of Winter Cereal and Upland Crops, Homan Agricultural Research Institute, NICS) ;
  • Kim Tae Wan (Department of Plant Resources Science, Hankyong National University)
  • Published : 2005.12.01
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Abstract

To investigate the application of biochemical markers' and small-sample methods using whole-wheat flours for screening in early generation in Korean wheat breeding system, 74 Korean wheats, including cultivars, local breeding lines and experimental lines, were analyzed. Seed storage protein and amylose contents of grains were evaluated. Biochemical makers, including granule bound starch synthase (GBSS), high molecular weigh glutenin subunits (HMW-GS) and friabilin were also evaluated by using one-dimensional sodium dodecyl sulfate-polyacryla-mide gel electrophoresis with a single kernel. The small­sample methods, including modified SDS-sedimentation test (MST), micro-alkaline water retention capacity (AWRC) and whole-wheat flour swelling volume (WSV) were also tested in this study. Protein content, MST and AWRC was $11.0 - 15.8\%$, 2.7 - 26.2 ml and $71.9 - 109.7\%$, respectively. Apparent and total amylose content and WSV was $20.6 - 25.0\%$, $26.1 - 32.4\%$ and 9.0 - 16.9 ml, respectively. There were highly significant correlations between MST and AWRC (r=0.592, P<0.001), but Korean wheats showed no significant difference in protein content, amylose content and small-sample methods. In the biochemical markers, Korean wheats contained all three GBSS encoded by Wx loci, except for Suwon 252. Korean wheats showed the high frequency ($58.1\%$) of 1Dx2.2 + 1Dy12 subunits of HMW-GS. Friabilin band was present in 46 lines ($62.2\%$) and absent in 28 lines ($37.8\%$). Friabilin-absence lines showed the higher MST (14.9 ml) and AWRC ($92.1\%$) value than friabilin-presence lines (8.5 ml and $82.4\%$, respectively).

Keywords

References

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