Antibacterial Activity of Topical Agent Extracted from Phellodendri cortex, Forsythia fructus and Glycyrrhizae radix Against Staphylococcus intermedius in Dogs

황백, 연교 및 감초 추출물을 이용한 국소제제의 Staphylococcus intermedius에 대한 항균효과

  • Jeong Hyo-Hoon (College of Veterinary Medicine, Kyungpook National University) ;
  • Oh Tae-Ho (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2005.12.01

Abstract

This study was performed to examine the antibacterial efecacy of topical agent extracted from Phellodendri cortex, Forsythia fructus and Glycyrrhizae radix as an alternative dermatological agent. Also, the most efficacious concentration of this agent and the skin-contact time inhibiting the vegetation of bacterial cells in various dilutions were determined. Five strains of Staphylococcus intermedius were exposed to various dilutions of this agent. The efficacy was assessed after exposure for time periods varying between 0 and 30 min. The antibacterial efficacy was shown to be the strongest at dilution of 1: 2. The number of viable organisms decreased significantly after 8 minutes of exposure time and was the smallest after 30 minutes of exposure time. This alternative dermatological agent is considered to be effective against Staphylococcus intermedius and has a potential to be a new topical agents far the treatment of pyoderma in dogs.

Keywords

References

  1. Berkelman RL, Holland BW, Anderson RL. Increased bactericidal activity of dilute preparations of povidone-iodine solutions. J Clin Microbiol 1982; 15: 635-639
  2. Cuellar MJ, Giner RM, Recio MC, Manez S, Rios JS. Topical anti-inflammatory activity of some Asian medicinal plants used in dermatological disorders. Fitoterapia 2001; 72: 221-229 https://doi.org/10.1016/S0367-326X(00)00305-1
  3. DeBoer JD. Strategies for management of recurrent pyoderma in dogs. Vet Clin North Am Small Anim Pract 1990; 20: 1509-1525
  4. Fukai T, Marumo A, Kaitou K, Kanda T, Terada S, Nomura T. Anti-Helicobacter pylori flavonoids from licorice extract. Life Sciences 2002; 71: 1449-1463 https://doi.org/10.1016/S0024-3205(02)01864-7
  5. Guaguere E. Topical treatment of canine and feline pyoderma. Vet Dermato 1996; 7: 145-151 https://doi.org/10.1111/j.1365-3164.1996.tb00239.x
  6. Higaki S, Hasegawa Y, Morohashi M. The correlation of kampo formulations and their ingredients on anti-bacterial activities against propionibacterium acnes. J Dermatol 1995; 22: 4-9
  7. Higaki S, Nakamura M, Morohashi M. Activity of eleven kampo formulations and eight kampo crude drugs against Propionibacterium acnes isolated from acne patients: retrospective evaluation in 1990 and 1995. J Dermatol 1996; 23: 871-875
  8. Hill PB, Moriello KA. Canine pyoderma. J Am Vet Med Assoc 1994; 204: 334-340
  9. Kitagawa S, Nishibe S, Baba H. Studies on the Chinese crude drug 'Forsythia fructus' . Yakugaku Zasshi 1987; 107: 274-278
  10. Koch HJ, Vercelli A. Shampoos and others topical therapies. In: Advances in Veterinary Dermatology Vol 2. Oxford: Pergamon Press. 1993: 409-411
  11. Kwochka KW. Recurrent pyoderma. In: Current Veterinary Dermatology. St. Louis: Mosby-year Book. 1993: 3-21
  12. Lemarie RJ, Hosgood G. Antiseptics and disinfectants in small animal practice. Compend Contin Educ Pract Vet 1995; 17: 1339-1351
  13. Lozier SM. Topical wound therapy. In: Surgical Complications and Wound Healing in the Small Animal Practice. Philadelphia: WB Saunders. 1993: 63-88
  14. Nagle TM, Torres SM, Horne KL, Grover R, Stevens MT. A randomized, double-blind, placebo-controlled trial to investigate the efficacy and safety of a Chinese herbal product (P07P) for the treatment of canine atopic dermatitis. Vet Dermatol 2002; 12: 265-274 https://doi.org/10.1046/j.0959-4493.2001.00267.x
  15. Nakamoto K, Sadamori S, Hamada T. Effects of crude drugs and berberine hydrochloride on the activities of fungi. J Prosthetic Dent 1990; 64: 691-694 https://doi.org/10.1016/0022-3913(90)90298-Q
  16. Scott DW, Miller WH, Griffin CE. Dermatologic therapy. In: Muller & Kirk's Small Animal Dermatology, 6th ed. Philadelphia: WB Saunders. 2001: 207-273
  17. Scott DW, Miller WH, Griffin CE. Bacterial skin diseases. In: Muller & Kirk's Small Animal Dermatology, 6th ed. Philadelphia: WB Saunders. 2001: 274-335
  18. Tanaka Y, Kikuzaki H, Fukuda H, Nakatani N. Antibacterial compounds of licorice against upper airway respiratory tract pathogens. J Nutr Sci Vitaminol 2001; 47: 270-273 https://doi.org/10.3177/jnsv.47.270
  19. Tsukiyama RI, Katsura H, Tokuriki N, Kobayashi M. Antibacterial activity of licochalcone A against spore-forming bacteria. Antimicrob Agents Chemother 2002; 46: 1226-1230 https://doi.org/10.1128/AAC.46.5.1226-1230.2002