Cloning of a Manganese Peroxidase cDNA Gene Repressed by Manganese in Trametes versicolor

  • Kim Yongho (Department of Microbiology, Division of Life Sciences, Kangwon National University) ;
  • Yeo Sumin (Department of Microbiology, Division of Life Sciences, Kangwon National University) ;
  • Kum Joohee (Department of Microbiology, Division of Life Sciences, Kangwon National University) ;
  • Song Hong-Gyu (Department of Microbiology, Division of Life Sciences, Kangwon National University) ;
  • Choi Hyoung T. (Department of Microbiology, Division of Life Sciences, Kangwon National University)
  • Published : 2005.12.01

Abstract

White-rot fungi have the following enzyme systems for lignin degradation: laccase, lignin peroxidase and manganese peroxidase. There are other types of peroxidases related to lignin degradation, one of which we have cloned a cDNA gene of manganese-repressed peroxidase (MrP) in Trametes versicolor isolated in South Korea. The mrp transcript level has been decreased by $1{\mu}M\;of\;Mn^{2+}$.

Keywords

References

  1. Collins, P.J., M.M. O'Brien, and A.D.W. Dobson. 1999. Cloning and characterization of a cDNA encoding a novel extracellular peroxidase from Trametes versicolor. Appl. Environ. Microbiol. 65, 1343-1347
  2. Han, M., H. Choi, and H. Song. 2004. Degradation of phenanthrene by Trametes versicolor and its laccase. J. Microbiol. 42, 94-98
  3. Johansson, T. and P.O. Nymann. 1996. A cluster of genes encoding major isozymes of lignin peroxidase and manganese peroxidase from the white-rot fungus Trametes versicolor. Gene 170, 31-38 https://doi.org/10.1016/0378-1119(95)00846-2
  4. Johansson, T., P.O. Nymann, and D. Cullen. 2002. Differential regulation of mnp2, a new manganese peroxidase-encoding gene from the lignolytic fungus Trametes versicolor PRL572. Appl. Environ. Microbiol. 68, 2077-2080 https://doi.org/10.1128/AEM.68.4.2077-2080.2002
  5. Jonsson, L., K. Sjostrom, I. Haggstrom, and P.O. Nymann. 1995. Characterization of a laccase gene from the white-rot fungus Trametes versicolor and structural features of basidiomycete laccases. Biochim. Biophys. Acta 1251, 210-215 https://doi.org/10.1016/0167-4838(95)00104-3
  6. Kim, Y., S. Cheong, S. Kim, H. Song, and H. Choi. 2003. Partial cloning of genes for lignin degrading enzymes in Trametes versicolor. Kor. J. Microbiol. 39, 201-205
  7. Kim, K., Y. Leem, K. Kim, K. Kim, and H. Choi. 2002. Transformation of the medicinal basidiomycete Trametes versicolor to hygromycin B resistance by restriction enzyme mediated integration. FEMS Microbiol. Lett. 209, 273-276 https://doi.org/10.1111/j.1574-6968.2002.tb11143.x
  8. Ruiz-Duenas, F.J., M.J. Martinez, and A.T. Martinez. 1999. Molecular characterization of a novel peroxidase isolated from the lignolytic fungus Pleurotus eryngii. Mol. Microbiol. 31, 223-235 https://doi.org/10.1046/j.1365-2958.1999.01164.x
  9. Schmidt, H.W.H., S.D. Haemmerli, H.E. Schoemaker, M.S.A. Leisola. 1989. Oxidative degradation of 3,4-dimethoxybenzyl alcohol and its methyl ether by the lignin peroxidase of Phanerochaete chrysosporium. Biochem. 28, 1776-1783 https://doi.org/10.1021/bi00430a053
  10. Shin, K.S. 2004. The role of enzymes produced by white-rot fungus Irpex lacteus in the decolorization of the textile industry effluent. J. Microbiol. 42, 37-41
  11. Wariishi, H., K. Valli, and M.H. Gold. 1989. Oxidative cleavage of a phenolic diarylpropane lignin model dimer by manganese peroxidase from Phanerochaete chrysosporium. Biochem. 28, 6017-6023 https://doi.org/10.1021/bi00440a044
  12. Wariishi, H., K. Valli, and M.H. Gold. 1991. In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium. Biochem. Biophys. Res. Comm. 176, 269-276 https://doi.org/10.1016/0006-291X(91)90919-X
  13. Wariishi, H., L. Akileswaran, and M.H. Gold. 1988. Manganese peroxidase from the basidiomycete Phanerochaete chrysosporium: spectral characterization of the oxidized states and the catalytic cycle. Biochem. 27, 5365-5370 https://doi.org/10.1021/bi00414a061