Antioxidant Activity and Total Phenolic Content of Callistemon citrinus Extracts

  • Park, Young-Ki (Div. Biotechnology, Korea Forest Research Institute) ;
  • Lee, Wi-Young (Div. Biotechnology, Korea Forest Research Institute) ;
  • Park, So-Young (Div. Biotechnology, Korea Forest Research Institute) ;
  • Ahn, Jin-Kwon (Div. Biotechnology, Korea Forest Research Institute) ;
  • Han, Mu-Seok (Div. Biotechnology, Korea Forest Research Institute)
  • Published : 2005.04.30

Abstract

Ethanol crude extracts of wood, bark, leaf, and fruit of Callistenwn citrinus were compared for their antioxidant activities based on DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical-scavenging activity and reducing power. Bark extract showed the most potent radical-scavenging activity and reducing power, showing 94.1 and 0.64% at 25 and $100\;{\mu}g/mL$, respectively. Total phenolic content of the bark extracts (275.0 mg GAE/g) was higher than those of others. Further fractionation of the bark extract using hexane, $CH_2Cl_2$, and EtOAc showed EtOAc fraction had the highest antioxidant activity ($IC_{50}\;6.7\;{\mu}g/mL$) and reducing power (0.82 at $100\;{\mu}g/mL$), with total phenolic content of 611.1 mg GAE/g. Total phenolic contents correlated with antioxidant activity ($R^2\;=\;0.7061$) and reducing power ($R^2\;=\;0.7399$).

Keywords

References

  1. Food Chem. v.83 Antioxidant activity and total phenolic content of Iranian Ocimum accessions Javanmardi, J.C.;Locke, S.E.;Vivanco, J.M.
  2. Food Chem. v.83 Antioxidant activity and total phenolic content of Iranian Ocimum accessions Javanmardi, J.C.;Locke, S.E.;Vivanco, J.M.
  3. Drug v.49 Prospects for the use of antioxidant therapies Maxwell, S.J.
  4. Antioxidants;Food Additives Sherwin, F.R.;Branen, R.(ed.)
  5. Food Chem. Toxicol. v.26 Safety evaluation of butylated hydroxyanisole from the prospective of effect on forestomach and oesphageal squamous epithelium Grice, H.C.
  6. Food Chem. Toxicol. v.24 Enhanced tumor development by butylated hydroxy-toluene (BHT) in the liver, lung and gastro-intestinal tract Witschi, H.P.
  7. J. Agric. Food Chem. v.49 Studies on the antioxidative activities of Hsian-tsao (Mesona Procumbens Hemsl) leaf gum Lai, L.S.;Chou, S.T.;Chao, W.W.
  8. Food Chem. v.81 Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L.) Urban Zainol, M.K.;Abd-Hamid, A.;Yusof, S.;Muse, R.
  9. Food Chem. v.85 Screening of radical scavenging activity of some medicinal and aromatic plant extracts Miliauskas, G.;Venskutonis, P.R.;Beek, T.A.V.
  10. P R Health Sci. J. v.17 Plants from Puerto Rico with anti-mycobacterium tuberculosis properties Frame, A.D.;Rios-Olivares, E.;De Jesus, L.;Ortiz, D.;Pagan, J.;Mendez, S.
  11. Pest Manag. Sci. v.57 Mesotrione: a new selective herbicide for use in maize Mitchell, G.;Bartlett, D.W.;Fraser, T.E.;Hawkes, T.R.;Holt, D.C.;Townson, J.K.;Wichert, R.A.
  12. J. Essent. Oil Res. v.10 Chemical composition of essential oil of Callistemon citrinus (Curtis) skeel from reunion Chane-Ming, J.;Vera, R.R.;Fraisse, D.J.
  13. KFRI J. For. Sci. v.65 The screening of antibacterial activity against Streptoccus mutans and glucosyltransferase inhibitory activity from woody plants Lee, W.Y.;Ahn, J.K.;Kwon, Y.J.
  14. Food Chem. v.81 Antioxidant activity and total phenolics of edible mushroom extracts Cheung, L.M.;Cheung, P.C.K.;Ooi, V.E.C.
  15. J. Korean Wood Sci. Technol. v.32 Structure-antioxidant activity relationships of isotlavonoids Park, Y.K.;Choi, D.H.;Lee, H.J.;Lee, S.S.;Lee, W.Y.;Ahn, J.K.
  16. Jpn. J. Nutr. v.44 Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine Oyaizu, M.
  17. Food Sci. Biotechnol. v.13 Antioxidant activity of organic solvent extracts from far infrared-treated rice hulls Lee, S.C.;Kim, J.H.;Jeong, S.M.;Ha, J.U.;Nam, K.C.;Ahn, D.U.
  18. Food Chem. v.79 Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp Siddhuraju, P.;Mohan, P.S.;Becker, K.
  19. J. Agric. Food Chem. v.43 Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves Meir, S.;Kanner, J.;Akiri, B.;Hadas, S.P.
  20. Food Chem. v.85 Antioxidant activity and phenolic compounds of Swiss chard (Beta vulgaris subspecies cycla) extracts Pyo, Y.H.;Lee, T.C.;Logendra, L.;Rogen, R.T.
  21. J. Agric. Food Chem. v.43 Antioxidant activity of various tea extracts in relation to their antimutagenicity Yen, G.C.;Chen, H.Y.
  22. Eur. Food Res. Technol. v.211 Radical scavenging by dietary flavonoids. A kinetic study of antioxidant efficiencies Madsen, H.L.;Andersen, C.M.;Jorgensen, L.V.;Skibsted, L.H.
  23. Eur. Food Res. Technol. v.211 Radical scavenging by dietary flavonoids. A kinetic study of antioxidant efficiencies Madsen, H.L.;Andersen, C.M.;Jorgensen, L.V.;Skibsted, L.H.
  24. Innov. Food Sci. Emerging Technol. v.2 Evaluation of bioactive properties of pollen extracts as functional dietary food supplement Kroyer, G;Hegedus, N.