Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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A Dipstick-Type Electrochemical Immunosensor for The Detection of The Organophosphorus Insecticide Fenthion

  • Cho, Young-Ae (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Cha, Geun-Sig (Department of Chemistry, Kwangwoon University) ;
  • Lee, Yong-Tae (Department of Biochemistry, and Institute of Natural Sciences, Yeungnam University) ;
  • Lee, Hye-Sung (Department of Food Science and Nutrition, Kyungpook National University)
  • Published : 2005.12.31
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Abstract

A dipstick-type immunochemical biosensor for the detection of the organophosphorus insecticide fenthion was developed using a screen-printed electrode system as an amperometric transducer with polyclonal antibodies against fenthion as a bioreceptor. The assay of the biosensor involved competition between the pesticide in the sample and pesticide-glucose oxidase conjugate for binding to the antibody immobilized on the membrane. This was followed by measurement of the activity of the bound enzyme by the supply of the enzyme substrate (glucose) and amperometric determination of the enzyme reaction product ($H_2O_2$). The activity of the bound enzyme was inversely proportional to the concentration of pesticide. The optimized sensor system showed a linear response against the logarithm of the pesticide concentration ranging from $10^{-2}$ to $10^3\;{\mu}g/L$.

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References

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