Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Isolation and Characterization of a Water-Soluble Polysaccharide from the Mycelia of Solid Cultured Phellinus linteus

  • Chung, Ha-Yull (Department of Food Science & Biotechnology, and Food & Biotechnical Research Center, Hankyong National University) ;
  • Kim, Tae-Wook (Department of Food Science & Biotechnology, and Food & Biotechnical Research Center, Hankyong National University)
  • Published : 2005.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Fraction (PMEx-AH-${\beta}$) of water-soluble polysaccharide, showing stimulating activity against macrophages, was isolated from mycelia of solid cultured Phellinus linteus by hot-water extraction, ethanol precipitation, and chromatography. Chemical characteristics of PMEx-AH-${\beta}$ were as follows: carbohydrate content, 71%; monosaccharide composition, Man:Glu:Gal (9:64:27); molecular weight, $1-7{\times}10^4$; uronic acid content, 6.8%. Fundamental structure of PMEx-AH-${\beta}$ is deduced as ${\beta}$-($1{$\rightarrow}6$)-D-glucan with ${\beta}$-($1{\rightarow}3$)-D-glucosidic side chains based on methylation analysis.

Keywords

References

  1. Protein Nucleic Acid Enz. v.21 Antitumor polysaccharides and host defence against cancer: a new way for cancer immunochemotherapy Maeda, Y.Y.;Ishimura, K.;Chihara, G.
  2. Chem. Pharm. Bull. v.43 B-lymphocyte stimulating polysaccharide from mushroom Phellinus linteus Song, K.S.;Cho, S.M.;Lee, J.H.;Kim, H.M.;Han, S.B.;Ko, K.S.;Yoo, I.D.
  3. Int. J. Immunopharmacol v.18 Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus Kim, H.M.;Han, S.B.;Oh, G.T.;Kim, Y.H.;Hong, D.H.;Hong, N.D.;Yoo, I.D.
  4. KSM News Letters v.12 A study on hypoglycemic effects of Phellinus linteus. mycelia in sreptozotocin induced diabetic rats Yang, B.K.;Kim, D.H.;Jeong, S.C.;Park, J.B.;Cho, S.P.;Hur, N.J.;Das, S.;Song, C.H.
  5. Korean J. Food Sci. Technol. v.32 Biological activities of polysaccharide extracted from the fruit body and cultured mycelia of Phellinus linteus 1Y001 Lee, J.W.;Baek, S.J.;Bang, K.W.;Kang, S.W.;Kang, S.M.;Kim, B.Y.;Ha, I.S.
  6. J. Korean Soc. Food Sci. Nutr. v.29 Antimutagenic and cytotoxicity effects of extracts Ji, J.H.;Kim, M.N.;Chung, C.K.;Ham, S.S.
  7. Biochem. Mol. BioI. Int. v.47 antitumor polysaccharide from the mycelium of liquid-cultured Agaricus blazei Murill Mizuno, M.;Minato, K.;Ito, H.;Kawade, M.;Terai, H.;Tsuchida, H.
  8. Food Sci. Biotechnol. v.13 The selective antitumor activity of water-soluble extracts of the fruiting bodies and the cultivated mycelia of Agaricus blazei Murill Kim, J.E.;Lee, W.S.;Chung, H.Y.;Jang, S.J.;Kim, J.S.;Lee, J.B.;Song, C.S.;Park, S.Y.
  9. Food Sci. Biotechnol. v.14 Isolation and characterization of a water-soluble polysaccharide from the mycelia of solid cultivated Agaricus blazei Murill Chung, H.Y.;Cho, Y.J.;Kim, T.
  10. Culturing process of mushroom mycelia using edible by-products Chung, H.Y.;Taekyong Nongsan Co.
  11. Biochem. Biophys. Res. Comm. v.197 Nitric oxide production from macrophages is regulated by arachidonic acid metabolites Imai, Y.;Kolb, H.;Burkart, V.
  12. Anal. Chem. v.28 Colorimetric method for determination of sugars and related substances Dubois, M.;Gilles, K.A.;Hamilton, J.K.;Rebers, P.A.;Smith, F.
  13. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry, O.H.;Rosebrough, N.J.;Farr, A.L.;Randall, R.J.
  14. Anal. Biochem. v.201 A colorimetric method for the quantitation of glucuronic acids and a specific assay for galacturonic acid Taylor, K.A.;Buchanan-Smith, J.G.
  15. Carbohydr. Res. v.103 Permethylation of polysaccharides by a modified Hakomori method Narui, T.;Takahashi, K.;Kobayashi, M.;Shibata, S.
  16. Carbohydr. Res. v.337 Structual characterization of a water-soluble ${\beta}$-D-glucan from fruiting bodies of Agaricus blazei Murill Dong, Q.;Yao, J.;Yang, X.T.;Fang, I.N.
  17. J. Microbiol. Biotechnol. v.6 Immunostimulating activitiy and characterizationation of polysaccharides from mycelium of Phellinus linteus Lee, J.H.;Cho, S.M.;Song, K.S.;Han, S.B.;Kim, H.M.;Hong, N.D.;Yoo, I.D.
  18. Cabohydr. Polym. v.12 Formolysis of a potent antitumor (1 ~6)-${\beta}$-glucan-protein complex from Agaricus blazei fruiting bodies and antitumor activity of the resulting products Kawagishi, H.;Kanao, T.;Inagaki, R.;Mizuno, T.
  19. Chem. Comm. v.8 A practical guide to the methylation analysis of carbohydrates Jansson, P.;Kenne, L.;Liedgren, H.;Lindberg, B.;Lonngren, J.
  20. Monosaccharides;Carbohydrate analysis Chaplin, M.F.; Chaplin, M.F.;Kennedy, J.F.(ed.)
  21. Cabohydr. Res. v.140 Fukuoka F. antitumor polysaccharide from P. ostreatus (FR.) QUEL.: isolation and structure ofa ${\beta}$-glucan Yoshioka, Y.;Tabeta, R.;Saito, H.;Uehara, N.