Journal of Trauma and Injury

The Korean Society of Traumatology (대한외상학회)
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The Effect of Methylene Blue on Inducible Nitric-oxide Synthase in a Rat Model of Acute Lung Injury Induced by Paraquat

파라쿼트를 투여한 백서의 급성 폐 손상 모델에서 메틸렌블루 투여가 Inducible Nitric Oxide Synthase 유전자 발현에 미치는 효과에 관한 연구

  • Park, Hyun Soo (Department of Emergency Medicine and Surgery, Seoul National University College of Medicine) ;
  • Lee, Chang Hyun (Department of Surgery, Seoul National University College of Medicine) ;
  • Jung, Sung Goo (Department of Emergency Medicine and Surgery, Seoul National University College of Medicine) ;
  • Suh, Gil Joon (Department of Emergency Medicine and Surgery, Seoul National University College of Medicine) ;
  • Jung, Sung Eun (Department of Surgery, Cheju National University College of Medicine) ;
  • Youn, Yeo Kyu (Department of Surgery, Seoul National University College of Medicine)
  • 박현수 (서울대학교 의과대학 응급의학교실) ;
  • 이창현 (서울대학교 의과대학 외과학교실) ;
  • 정성구 (서울대학교 의과대학 응급의학교실) ;
  • 서길준 (서울대학교 의과대학 응급의학교실) ;
  • 정성은 (제주대학교의과대학 외과학교실) ;
  • 윤여규 (서울대학교 의과대학 외과학교실)
  • Received : 2005.05.16
  • Accepted : 2005.06.20
  • Published : 2005.06.30
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Abstract

Purpose: This study was designed to determine if methylene blue inhibited the lipid peroxidation, the production of NO, and the gene expression of iNOS in acute lung injury induced by paraquat and if the inhibitory effect was dose dependent. Methods: Female Sprague-Dawley rats were divided into four groups: the control group, the group treated with paraquat only, the group treated with paraquat and a low dose of methylene blue (2 mg/kg), and the group treated with paraquat and a high dose of methylene blue (20 mg/kg). Methylene blue was administered via the jugular vein 1 h after paraquat administration, and animals were sacrificed 6 and 24 h after paraquat administration. Malondialdehyde (MDA) as lipid peroxidation, reduced glutathione (GSH) as an antioxidant defense, the plasma NO concentration, and the expression of iNOS mRNA in the lung tissue were measured Results: Lung MDA contents decreased, with no significant difference between the methylene-blue groups and the paraquat-only group. Lung GSH contents were significantly elevated at 24 h in the methylene-blue groups compared with the paraquat-only group. Plasma NO concentrations were significantly reduced at 6 and 24 h in the methylene-blue groups compared with the paraquat-only group. There was also a significant decrease in the plasma NO concentration at 6 h in the high-dose methylene-blue group compared with the low-dose methylene-blue group. The expression of iNOS mRNA in the lung tissue was slightly decreased in the methylene-blue groups. It was also markedly increased at 24 h in the paraquat-only group compared with the methylene-blue groups. The gene expression was relatively decreased in the high-dose methylene-blue group compared with the low-dose methylene-blue group. Conclusion: This study suggests that methylene blue has an inhibitory effect on the plasma NO concentration and the expression of iNOS mRNA in lung injury induced by paraquat. No inhibitory effect of methylene blue on lipid peroxidation or dose-dependent inhibitory effects were clearly shown.

Keywords

References

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