Journal of Apiculture (한국양봉학회지)

The Apicultural Society of Korea (한국양봉학회)
권호 표지

Identification of Deformed Wing Virus from the Honeybee in Korea and Establishment of PCR Detection Method

한국산 꿀벌에서 Deformed Wing Virus의 발견과 Real-Time PCR 검출법의 확립

Lee, Hye-Min;Lee, Do-Bu;Han, Sang-Hoon;Lee, Myeong-Lyeol;Lim, Yoon-Kyu;Yoon, Byoung-Su
이혜민;이도부;한상훈;이명렬;임윤규;윤병수

  • Published : 20051200
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Abstract

Deformed wing virus (DWV) causes a wing deformity and a premature death of an adult honeybee. Although those symptoms were found in korean honeybees, this is first report, in our knowledge, that the infection the DWV have been presented in Korea. Rapid detection system may be needed for the management of hive because DWV generally persists as a latent infection with no apparent symptom and there is no available control method for this disease yet. In this study, our laboratory confirmed the presence of the DWV using PCR detection method from adult honeybees which have wing deformities. PCR product size is 479 base pairs with a specific primer set(DWV-SF/DWV-SR). With T-vector cloning, the sequence of the PCR product was analyzed. And then, Real-Time PCR detection method for a rapid detection of DWV was developed. The PCR system was able to detect easily the existence of the virus from honeybee sample. Specific PCR method showed improved sensitivity in the artificially contaminated templates.

Keywords

References

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