Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
권호 표지

The chemical composition and biological activities of volatile flavor components of Elsholtzia splendens

꽃향유 전초의 향기성분 분석과 생리활성 평가

  • Jeong, Jae Hoon (Department of Industrial Plant Science, Colleage of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Lim, Heung Bin (Department of Industrial Plant Science, Colleage of Agriculture, Life & Environment Science, Chungbuk National University)
  • 정재훈 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 임흥빈 (충북대학교 농업생명환경대학 특용식물학과)
  • Received : 2005.07.12
  • Accepted : 2005.10.24
  • Published : 2005.12.26
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Abstract

This study was conducted to investigate the chemical composition of essential oil, absolute and oleoresin isolated from Elsholtzia splendens and their biological activities. Yields of essential oil, absolute and oleoresin extracted from Elsholtzia splendens were 0.28%, 12.45% and 9.95%, respectively. The major component was 2-cyclohexen-1-one in essential oil, methyl linolenate in absolute and 9,12,15-octadecatrienoic acid in oleoresin. Essential oil and oleoresin showed the inhibitory activities in enzyme-dependent, enzyme-independent and autooxidative lipid peroxidation system. $EC_{50}$ values in nuetral red uptake (NRU) assays for the exposure times of 24 h were $46.4{\mu}g/ml$, $681.7{\mu}g/ml$ and $17.6{\mu}g/ml$ in essential oil, absolute and oleoresin, and oleoresins showed the cytotoxic effect at the only high dose. Any mutagenic and antibiotic activity did not show in absolute and oleoresin, but, there were mutagenic and antibiotic activities only when treated with essential oil $500{\mu}g/ml$ above in Ames test. Essential oil and oleoresin might be somewhat effective in prolongating the ciliostasis of rat trachea.

향료자원 꽃향유 전초의 향기성분 essential oil, absolute 및 oleoresin을 추출 분리 동정하고, 지질과산화 억제, 세포독성, 돌연변이유발성 및 거담효과 등의 생리활성을 측정하였다. 꽃향유 전초의 essential oil 수율은 0.28%이었으며, 2-cyclohexen-1-one이 26.81%와 elsholtizia ketone이 13.46%로 가장 많이 함유되어 있었다. 꽃향유 전초의 absolute의 수율은 12.45%이었으며, methyl linolenate가 12.38%이었고, palmitic acid 9.47% 비율로 존재하고 있었다. 꽃향유 전초의 oleoresin의 수율은 9.95%이었으며, 9,12,15-octadecatrienoic acid가 22.15%로 가장 높은 비율을 차지하고 있었다. 세 방법의 지질과산화 억제활성 시험에서 $200{\mu}g/ml$ 처리했을 때 꽃향유전초의 essential oil과 oleoresin은 지질과 산화를 잘 억제하였으나, absolute는 거의 억제하지 못하였다. 꽃향유 전초의 oleoresin은 24시간에서 $EC_{50}$값이 $17.6{\mu}g/ml$로 고농도에서만 약간의 세포독성이 있으며, absolue와 oleoresin은 고 농도에서도 돌연변이 유발성과 항균성이 나타나지 않았으나 essential oil은 $500{\mu}g/ml$이상 처리 시 돌연변이 유발성 뿐만 아니라 항균성도 있는 것으로 나타났다. 꽃향유 전초의 essential oil과 oleoresin은 약간의 거담효과는 있을 것으로 판단된다.

Keywords

Acknowledgement

Supported by : 충북대학교

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