Journal of The Korean Ophthalmological Society (대한안과학회지)

The Korean Ophthalmological Society (대한안과학회)
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The Effect of Pinitol on Cataractogenesis and Anti-oxidative effect in Streptozotocin Induced Diabetic Rats

피니톨이 당뇨쥐에서 백내장 및 항산화계에 미치는 영향

Park, Jong-Hwa;Lee, Youn-Jung;Kim, Jong-Jin;Shin, Yong-Chul;Kim, Jae-Chan
박종화;이윤정;김종진;신용철;김재찬

  • Published : 20051100
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Abstract

Purpose: Pinitol, a natural extract of soybeans, is metabolized to chiroinositol in the body. We evaluated the ability of pinitol to protect diabetic cataracts from oxidative stress in streptozotocin (STZ)-induced diabetic rats. Methods: We used Sprague-Dawley rats (n=80). Diabetes was induced with STZ (60 mg/kg, i.p.). These STZ-injected rats were administered pinitol or chiroinositol, each 20 mg/$m\ell$, and given access to regular chow. The efficacies of pinitol and chiroinositol were studied by monitoring a series of lens opacity and corneal lesion by photodocumentation. Aqueous humor and lens samples were collected at the 2nd week, 4th week, and 12th week. We measured glutathione, malondialdehyde (MDA) and used ELISA to evaluate its antioxidant effect. Results: STZ-diabetic rats showed typical Y sutural lens opacity beginning on the 3rd day and progressed diffusely to more cortical opacity from the 1st week. However, in pinitol-treated diabetic rats, these cataractous changes were remarkably decreased. Corneal edema and opacity also remarkably reduced in the pinitol-treated group. Glutathione level markedly increased compared to that of the non-treated diabetic group (230.12$\pm$10.96 ${\mu}m$ , 156.42$\pm$5.09 ${\mu}m$, respectively). This resulted in a decrease in peroxidized MDA product in the treated group. Similarly, the anti-cataractogenic and anti-oxidative effects of pinitol were also observed in the chiroinositol-treated group. Conclusions: These results suggest that pinitol could be effective in preventing cataract and cornea edema caused by oxidative stress in a hyperglycemic environment.

목적 : STZ를 이용한 실험적 당뇨병 모델에서 대두에서 분리한 pinitol (3-O-methyl-D-chiroinositol)을 투여한 후 수정체와 항산화 효소계에 미치는 영향을 알아보고자 하였다. 대상과 방법 : 80마리의 흰 쥐를 실험대상으로 하여 STZ를 투여하여 당뇨를 유도한 후 4개의 군으로 나누었다: A군(정상 대조군), B군(STZ로 당뇨병을 유도한 군), C군(STZ로 당뇨병 유도후 CI를 투여한 군), D군(STZ로 당뇨병 유도후 PI를 투여한 군). 그 후 12주까지 각막의 상태 및 수정체 혼탁의 진행 양상을 시간에 따라 관찰하였으며, 2, 4, 12주에는 실험동물의 수정체와 방수에서 GSH와 MDA의 농도 및 AGE의 발현을 측정하여 각 군간의 차이를 비교하였다. 결과 : C군과 D군에서 각막 부종과 수정체 혼탁의 진행이 B군보다 적었고, GSH와 MDA의 농도도 정상 대조군(A군)에 비슷한 결과를 나타내었으며 그 효과는 D군에서 보다 높게 나타났다. AGE의 발현은 관찰되지 않았다. 결론 : STZ로 유발된 당뇨병 모델에서 pinitol이 항산화 효소계에 영향을 미치며 각막 부종 및 수정체 혼탁의 발생과 진행을 억제함을 알 수 있었다.

Keywords

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