Journal of Apiculture (한국양봉학회지)

The Apicultural Society of Korea (한국양봉학회)
권호 표지

Studies on the Antimicorbial Effect of Collected Bee Venom Using Electric Shock Method (Ⅰ)

전기충격법으로 채취한 국내산 봉독의 향균 효과 (Ⅰ)

Han, Sang-Mi;Lee, Kwang-Gill;Yeo, Joo-Hong;Kweon, Hae-Yong;Woo, Soon-Ok;Lee, In-Kyung;Lee, Myeong-Lyeol;Lee, Man-Young;Baek, Ha-Ju;Bae, Ki-Hwan
한상미;이광길;여주홍;권해용;우순옥;이인경;이명렬;이만영;백하주;배기환

  • Published : 20050600
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Abstract

This study was carried out to evaluate antimicrobial effects of bee venom. We collected bee venom using bee venom collector devices that use electrical impulses to stimulate the bees to sting. We demonstrated that the component of collected bee venom and bee venom standards is similar in its composition. The antimicrobial activity against Bacillus subtilis, Salmonella typhimurium, Staphylococcus aureus and Streptococcus mutans was investigated. The minimal inhibitory concentration (MIC) of the bee venom against microorganisms were also examined. Antimicrobial activities of amocla as references were compared to those of bee venom. The antimicrobial activity of bee venom had growth inhibition activity against gram-negative (MIC ＜ 5 ${\mu}g/ml$) and-positive (MIC ＜ 10 ${\mu}g/ml$) bacteria. These results suggest that the Korean bee venom may be a valuable choice for the study on the growth inhibition activity of microorganisms.

Keywords

References

  1. 이건섭. 1996. 진단병원미생물학. 고려의학 pp 589-601
  2. Abbadie, C. and J. M. Besson. 1994. Chronic treatments with aspirin of acetaminophen reduce both the development of polyarthrithritis and Fos-like immunoreactivity in rat lumbar spinal cord. Pain. 57:45-54 https://doi.org/10.1016/0304-3959(94)90106-6
  3. Chen, J., C. Lou and H. L. Li. 1998. The contribution of spinal neuronal changes to development of prolonged, tonic nociceptive responses of the cat induced by subcutaneous bee venom injection. Eur J Pain 2:359-376 https://doi.org/10.1016/S1090-3801(98)90034-9
  4. Choi, S H., S K. Cho, S S. Kang, C S. Bae, Y H. Bai, S H. Lee and S C. Pak. 2003. Effect of apitherapy in piglets with preweaning diarrhea. The American Journal of Chinese Medicine. 31: 321-326 https://doi.org/10.1142/S0192415X03001004
  5. Dotimas, E. M. and R. C. Hider. 1987. Honeybee venom. Bee World 68:51-70 https://doi.org/10.1080/0005772X.1987.11098915
  6. Dunn, J. D. and J. J. Killion. 1988. Effect of melittin on pituitary adrenal responsiveness to stress. Acta. Endorinol. 119: 339-344
  7. Gauldie, J., J. M. Hanson, R. A. Shipolini, C. A. Vernon. 1978. The structures of some peptides from bee venom. Em J Biochem. 83:405-410 https://doi.org/10.1111/j.1432-1033.1978.tb12106.x
  8. Habermann, E. and K. G. Reiz. 1965. On the biochemistry of bee venom peptides, melittin and apamin. Biochem. 343:192-203
  9. Kim, H W., S D. Park, A R. Lee, J. H. Jang, K H. Kim and K S. Kim. 2002. The effect of bee venom acupuncture on protease activity and free radical damage in synovial fluid from collagen-induced rthritis in rats. The Korean Acupuncture and Moxibustion Society. 19:161-175
  10. Kim, H W., Y. B. Kwon, T W. Ham, D. H. Rho, S. Y. Yoon, H. J. Lee, H. J. Han, J. S. Yang, A J. Beitz and J. H. Lee. 2003. Acupoint stimulation using bee venom attenuates formalin-induced pain behavior and spinal cord for expression in rats. The Journal of Veterinary Medical Science. 65: 349-355 https://doi.org/10.1292/jvms.65.349
  11. Kwon, Y B., J. D. Lee, H. J. Lee, J. J. Han, W. C. Mar, S. K. Kang, A. J. Beitz and J. H. Lee. 2001. Bee venom injection into an acupuncture point reduces arthritis associated edema and nociceptive responses. Pain. 90:271-280 https://doi.org/10.1016/S0304-3959(00)00412-7
  12. Lin, J. H. and P. A. M. Rogers. 1980. Acupuncture effects on the body's defense systems. A veterinary Review. Vet Bulle. 50:633-640
  13. Rudenko, S. V. and E. E. Nipot. 1996. Modulation of melittin-induced hemolysis of erythrecytes. Biokhimiia. 61:2116-2124
  14. Shkenderov, S. H. 1982. Adolapin-a newly analgetic and antiinflammatory polipeptide from bee venom. Toxicon. 20:317-321 https://doi.org/10.1016/0041-0101(82)90234-3
  15. Sprott, H., S. Franke, H. Kluge and G. Hein. 1998. Pain treatment of fibromyalgia by acupuncture. Rheumatol Int. 18:35-36 https://doi.org/10.1007/s002960050051
  16. Vick, J. A., B. Brooks and W. Shipmane. 1972. Therapeutic applications of bee venom and its components in the dog. Am Bee J. 11:414-416