Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Extraction of Whitening Agents from Natural Plants and Whitening Effect

천연물에 포함된 미백성분의 추출 및 미백효과

  • Jin, Yinzhe (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Ahn, So Young (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Hong, Eun Suk (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Li, Guang hua (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Kim, Eun-Ki (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University) ;
  • Row, kyung Ho (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University)
  • 김은철 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부) ;
  • 안소영 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부) ;
  • 홍은숙 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부) ;
  • 이광화 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부) ;
  • 김은기 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부) ;
  • 노경호 (초정밀생물분리기술연구센터, 인하대학교 생명화학공학부)
  • Received : 2004.10.19
  • Accepted : 2005.02.22
  • Published : 2005.06.10
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Abstract

The extracts from natural and fermented products such as Artemisia plants, Rhodiola Salientness, fermented soybeans and soybean paste were used to investigate the whitening effect. 10 g of Artemisia plant were added to 300 mL of ethanol and extracted by sonification at room temperature for 3 h. The extract was further partitioned by the equal volume percent in the order of the n-hexane, chloroform and ethyl acetate. 5 g of Rhodiola salientness was also added to 150 mL of methanol and extracted at the room temperature for 12 h. The effluents from a chromatographic column ($3.9{\times}250mm$, $C_{18}$, $15{\mu}m$) were collected and concentrated in two parts. The extraction of fermented soybeans and soybean paste were done by 60% ethanol. In this work, tyrosinase inhibitory activity and melanin inhibitory effect were measured to confirm the whitening effect. The water layer of Artemisia princeps Pampan showed the good inhibitory of antioxidant, while the hexane layer of Artemisia iwayomogi Kitamura and the chloroform layer of Artemisia princeps Pampan had the excellent melanin inhibitory effect. The Rhodiola salientness had the superior whitening effect to the arbutin in in-vivo melanin production ratio assay. However, the fermented soybeans and soybean paste did not show any whitening effect.

천연물 중에서 사자발쑥과 인진쑥, 홍경천, 청국장, 된장 추출물로 미백효과를 측정하였다. 쑥 10 g에 300 mL의 에탄올로 상온에서 3 h의 초음파 추출을 진행하였다. 추출물을 차례로 같은 양의 헥산, 클로로포름, 에틸아세테트로 분배과정을 거쳤다. 홍경천 5 g에 150 mL의 메탄올을 첨가하고 상온에서 12 h을 냉침하여 얻은 추출물을 액체 크로마토그래피를 통과하였다. 제조용 컬럼($3.9{\times}250mm$, $C_{18}$, $15{\mu}m$)을 통하여 두개 부분의 분획물을 획득할 수 있었다. 청국장과 된장은 60% 에탄올을 사용하여 추출하였다. 티로시나아제 억제활성과 멜라닌 억제효과로 미백효과를 측정하였다. 쑥의 경우 항산화효과에서는 인진쑥의 물층, 멜라닌 억제 효과에서는 사자발쑥의 헥산층, 인진쑥의 클로로포름층이 우수하였다. 홍경천 추출물은 in-vivo melanin production ratio assay에서 melanin-a 세포를 사용한 결과 미백효과는 알부틴보다 우수하였다. 청국장과 된장의 60% 에탄올 추출물은 미백효과를 나타내지 않았다.

Keywords

Acknowledgement

Supported by : 인하대학교

References

  1. G. C. Romero, et al., J. Clin. Invest. 99, 635 (1997) https://doi.org/10.1172/JCI119206
  2. A. L. Soon and S. J. Lee, Korea Beautymagazine, 6, 239 (2000)
  3. K. Maeda and M. Fukuda, J. Soc. Cosmet. Chem., 42, 361 (1991)
  4. Y. Mishima, S. Hatae, and H. Kondon, Fragrance J., 1, 13 (1996)
  5. K. H. Jung and M. S. Thesis, Konkuk University, Seoul, Korea (2002)
  6. S. J. Lee, Kor. J. Raw Med. 6, 75 (1975)
  7. 농촌진흥청, 표준 영농교본, 산채류재배, 88 (1990)
  8. D. L. Morris, Science 107, 254 (1948) https://doi.org/10.1126/science.107.2775.254
  9. L. N. Han, H. O. Yang, and B. H. Han, Medicinemagazine, 28, 69 (1984)
  10. Y. H. Kim, Master Thesis, Hanyang Univ., Seoul, Korea (1981)
  11. S. A. Moon, Master Thesis, Dongduk Univ., Seoul, Korea (1993)
  12. K. S. Kim, Korea Veterinary medicine, 35, 489 (1995)
  13. S. Jung and D. Y. Kang, Chungnam Medicalmagazine, 12, 27 (1985)
  14. U. K. Han, Ewha Medicalmagazine, 22, 57 (1983)
  15. 한규석, 강원도보건환경연구원보, 6, 51 (1995)
  16. Y. K. Han, Master Thesis, Seoul National Univ., Seoul, Korea (1999)
  17. http://my.netian.com/-asia-co/a01.html
  18. J. D. Laskin and L. A. Piccinini, J. Biol. Chem. 261, 16226 (1986)
  19. M. S. Blosi, Nature, 181, 1199 (1958) https://doi.org/10.1038/1811199a0
  20. S. H. Lee, S. Y. Chol, H. C. Kim, J. S. Hwang, B. G. Lee, J. J. Gao, and S. Y. Kim, Biol. Pharm. Bull. 25, 1045 (2002) https://doi.org/10.1248/bpb.25.1045