Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of Novel SNPs in Bovine Insulin-like Growth Factor Binding Protein-3 (IGFBP3) Gene

  • Kim, J.Y. (Department of Genetic Epidemiology, SNP Genetics, Inc.) ;
  • Yoon, D.H. (National Livestock Research Institute, RDA) ;
  • Park, B.L. (Department of Genetic Epidemiology, SNP Genetics, Inc.) ;
  • Kim, L.H. (Department of Genetic Epidemiology, SNP Genetics, Inc.) ;
  • Na, K.J. (National Livestock Research Institute, RDA) ;
  • Choi, J.G. (National Livestock Research Institute, RDA) ;
  • Cho, C.Y. (National Livestock Research Institute, RDA) ;
  • Lee, H.K. (Department of Biotechnology, Hankyong National University) ;
  • Chung, E.R. (Department of Biotechnology, Sangi University) ;
  • Sang, B.C. (Department of Dairy Science, Graduate School, Chungnam National University) ;
  • Cheong, I.J. (National Livestock Research Institute, RDA) ;
  • Oh, S.J. (National Livestock Research Institute, RDA) ;
  • Shin, Hyoung Doo (Department of Genetic Epidemiology, SNP Genetics, Inc.)
  • Received : 2003.09.08
  • Accepted : 2004.02.09
  • Published : 2005.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

The insulin-like growth factors (IGFs), their receptors, and their binding proteins play key roles in regulating cell proliferation and apoptosis. Insulin-like growth factor binding protein-3 (IGFBP3, OMIM #146732) is one of the proteins that bind to the IGFs. IGFBP3 is a modulator of IGF bioactivity, and direct growth inhibitor in the extravascular tissue compartment. We identified twenty-two novel single nucleotide polymorphisms (SNPs) in IGFBP3 gene in Korean cattle (Hanwoo, Bos taurus coreanae) by direct sequencing of full gene including -1,500 bp promoter region. Among the identified SNPs, five common SNPs were screened in 650 Korean cattle; one SNP in promoter (IGFBP3 G-854C), one in 5'UTR region (IGFBP3 G-100A), two in intron 1 (IGFBP3 G+421T, IGFBP3 T+1636A), and one in intron 2 (IGFBP3 C+3863A). The frequencies of each SNP were 0.357 (IGFBP3 G-854C), 0.472 (IGFBP3 G-100A), 0.418 (IGFBP3 G+421T), 0.363 (IGFBP3 T+1636A) and 0.226 (IGFBP3 C+3863A), respectively. Haplotypes and their frequencies were estimated by EM algorithm. Six haplotypes were constructed with five SNPs and linkage disequilibrium coefficients (|D'|) between SNP pairs were also calculated. The information on SNPs and haplotypes in IGFBP3 gene could be useful for genetic studies of this gene.

Keywords

References

  1. Baxter, R. C. 1993. IGF binding protein-3 and the acid-labile subunit: formation of the ternary complex in vitro and in vivo. Adv. Exp. Med. Biol. 343:237-244.
  2. Clemmons, D. R. 1993. IGF binding proteins and their functions. Mol. Reprod. Dev. 35(4):368-74; discussion 374-375. https://doi.org/10.1002/mrd.1080350409
  3. Clemmons, D. R. 1997. Insulin-like growth factor binding proteins and their role in controlling IGF actions. Cytokine Growth Factor Rev. 8(1):45-62. https://doi.org/10.1016/S1359-6101(96)00053-6
  4. Collett-Solberg, P. F. and P. Cohen. 1996. The role of the insulinlike growth factor binding proteins and the IGFBP proteases in modulating IGF action. Endocrinol. Metab. Clin. North Am. 25(3):591-614.
  5. D'Ercole, A. J. 1987. Somatomedins/insulin-like growth factors and fetal growth. J. Dev. Physiol. 9(6):481-495.
  6. Ferry, R. J., Jr., R. W. Cerri and P. Cohen. 1999. Insulin-like growth factor binding proteins: new proteins, new functions. Horm. Res. 51(2):53-67.
  7. Ferry, R. J., Jr., L. E. Katz, A. Grimberg, P. Cohen and S. A. Weinzimer. 1999. Cellular actions of insulin-like growth factor binding proteins. Horm. Metab. Res. 31(2-3):192-202.
  8. Hedrick, P. W. 1987. Gametic disequilibrium measures: proceed with caution. Genetics 117(2):331-341.
  9. Jones, J. I. and D. R. Clemmons. 1995. Insulin-like growth factors and their binding proteins: biological actions. Endocr. Rev. 16(1):3-34.
  10. Kappes, S. M., J. W. Keele, R. T. Stone, R. A. McGraw, T. S. Sonstegard, T. P. Smith, N. L. Lopez-Corrales and C. W. Beattie. 1997. A second-generation linkage map of the bovine genome. Genome. Res. 7(3):235-249.
  11. Liu, F., D. R. Powell and R. L. Hintz. 1990. Characterization of insulin-like growth factor-binding proteins in human serum from patients with chronic renal failure. J. Clin. Endocrinol. Metab. 70(3):620-628.
  12. Maciulla, J. H., H. M. Zhang and S. K. DeNise. 1997. A novel polymorphism in the bovine insulin-like growth factor binding protein-3 (IGFBP3) gene. Anim. Genet. 28(5):375.
  13. Oh, Y., H. L. Muller, G. Lamson and R. G. Rosenfeld. 1993. Insulin-like growth factor (IGF)-independent action of IGFbinding protein-3 in Hs578T human breast cancer cells. Cell surface binding and growth inhibition. J. Biol. Chem. 268(20):14964-14971.
  14. Phillips, L. S., C. I. Pao and B. C. Villafuerte. 1998. Molecular regulation of insulin-like growth factor-I and its principal binding protein, IGFBP3. Prog. Nucleic Acid Res. Mol. Biol. 60:195-265.
  15. Ryu, Y., J. Yang, D. Seo, and Y. Ko. 2003. Effect of Serum IGF-I on Progesterone Concentration during Early Pregnancy in Korean Native Cattle (Hanwoo). Asian-Aust. J. Anim. Sci. 16(1):176-179.
  16. Stewart, C. E. and P. Rotwein. 1996. Growth, differentiation, and survival: multiple physiological functions for insulin-like growth factors. Physiol. Rev. 76(4):1005-1026.
  17. Thissen, J. P., J. M. Ketelslegers and L. E. Underwood. 1994. Nutritional regulation of the insulin-like growth factors. Endocr. Rev. 15(1):80-101.
  18. Thue, T. D. and F. C. Buchanan. 2002. A new polymorphism in the cattle IGFBP3 gene. Anim. Genet. 33(3):242.
  19. Valentinis, B., A. Bhala, T. DeAngelis, R. Baserga and P. Cohen. 1995. The human insulin-like growth factor (IGF) binding protein-3 inhibits the growth of fibroblasts with a targeted disruption of the IGF-I receptor gene. Mol. Endocrinol. 9(3):361-367.

Cited by

  1. Identification of Functional and In silico Positional Differentially Expressed Genes in the Livers of High- and Low-marbled Hanwoo Steers vol.20, pp.9, 2005, https://doi.org/10.5713/ajas.2007.1334
  2. DdeI Polymorphism in Coding Region of Goat POU1F1 Gene and Its Association with Production Traits vol.20, pp.9, 2007, https://doi.org/10.5713/ajas.2007.1342
  3. Association Analysis between Insulin-like Growth Factor Binding Protein 3 (IGFBP3) Polymorphisms and Carcass Traits in Cattle vol.21, pp.3, 2005, https://doi.org/10.5713/ajas.2008.60476
  4. Single nucleotide polymorphism in Egyptian cattle insulin-like growth factor binding protein-3 gene vol.12, pp.2, 2014, https://doi.org/10.1016/j.jgeb.2014.07.001