Journal of Apiculture (한국양봉학회지)

The Apicultural Society of Korea (한국양봉학회)
권호 표지

Real-Time PCR을 이용한 백묵병 원인균 Ascosphera apis의 신속 검출

Lee, Hye-Min;Lee, Do-Bu;Han, Sang-Hoon;Nam, Seoung-Hee;Lim, Yoon-Kyu;Yoon, Byoung-Su
이혜민;이도부;한상훈;남성희;임윤규;윤병수

  • Published : 20051200
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Abstract

Chalkbrood is a well known fungal disease of honeybees, that was caused by a fungus, Ascosphaera apis. Because there is no available control method for disease, and because co-infection with other pathogens could be induced a rapid detection system will be important in a hive management. In this study, Real-time PCR method for the rapid detection of chalkbrood was developed. A specific primer-pair was designed based on 185 rRNA gene of Ascosphaera apis. This PCR could easily detect the existence of the pathogen from larvae samples. In addition, the minimum concentration of template DNA was about 6 copies. From this result, shortening the detection time in Chalkbrood disease could induce a strong advance in this field.

Keywords

References

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