Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Muscle Proteome Analysis for the Effect of Panax Ginseng Extracts in Chicken: Identification of Proteins Using Peptide Mass Fingerprinting

  • Jung, K.C. (1Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Yu, S.L. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Lee, Y.J. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Choi, K.D. (The Graduate School of Bio and Information Technology, Hankyong National University) ;
  • Choi, J.S. (Proteome Analysis Team, Korea Basic Science Institute) ;
  • Kim, Y.H. (Proteome Analysis Team, Korea Basic Science Institute) ;
  • Jang, B.G. (National Livestock Research Institute, R.D.A.) ;
  • Kim, S.H. (Department of Biological Science, Kyunghee University) ;
  • Hahm, D.H. (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, J.H. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University)
  • Received : 2004.09.22
  • Accepted : 2005.02.18
  • Published : 2005.07.01
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Abstract

The present study was aimed to investigate proteome affected by Panax ginseng extracts in chicken muscles. The whole muscle proteins from chicken fed boiled extracts of 0% (control), 1%, 3%, and 5% Panax ginseng in water were separated by two-dimensional electrophoresis (2-DE) gels using immobilized non-linear gradient (pH 3-10) strips. More than 300 protein spots were detected on silver staining gels. Among them, four protein spots were distinctively up-regulated by Panax ginseng treatments and further investigated by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The obtained MS data were searched against SwissProt database using the Mascot search engine. The up-regulated proteins were finally identified as $\alpha$-tropomyosin (2 spots), triosephosphate isomerase, and one unknown protein. Based on the known functions of the identified proteins, they are highly related to muscle development and enhanced immunity in chickens. These proteins can give valuable information of biochemical roles for Panax ginseng in chicken meats.

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References

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