Clinical and Molecular Hepatology (대한간학회지)

The Korean Association for the Study of the Liver (대한간학회)
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Prostaglandin $A_2$ Induces Caspase-independent Apoptosis in Hepatocellular Carcinoma Cells

Prostaglandin $A_2$에 의한 간세포암종 세포의 Caspase-비의존적 Apoptosis 유도

Kim, Ho-Shik;Shim, Jae-Chun;Choi, Ju-Youn;Rhim, Hyang-Shuk;Kim, In-Kyung
김호식;심재천;최주연;임향숙;김인경

  • Published : 20050300
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Abstract

Background/Aims: Prostaglandin (PG) $A_{2}$ has been reported to inhibit the growth of hepatocellular carcinoma cells via activation of apoptosis, although the molecular mechanisms involved have not been clarified, yet. To investigate the mechanism of the $PGA_{2}$-induced apoptosis, we analyzed the activation of caspases during the apoptosis of hepatoma cell lines. Methods: Induction of apoptosis by $PGA_{2}$ in hepatoma cell lines, Hep 3B and Help G2, was assessed by DAPI staining of nuclei and agarose gel electrophoresis of genomic DNA. The involvement of caspases was analyzed by immunoblot analysis of poly ADP-ribose polymerase (PARP) and by checking the effect of caspase inhibitors on $PGA_{2}$-induced apoptosis. Results: $PGA_{2}$ inhibited the growth of Hep 3B and Hep G2 cells, accompanying nuclear condensation and fragmentation, and genomic DNA laddering, which are the hallmarks of apoptosis. The PARP was not cleaved during the apoptosis of Hep 3B and Hep G2 cells and caspase inhibitors such as z-VAD-Fmk and z-DEVD-Fmk exerted no effect on the $PGA_{2}$-induced apoptosis. Conclusions: These results suggest that $PGA_{2}$ induces apoptosis in Hep 3B and Hep G2 cells via caspase-independent pathway.

Keywords

References

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