DOI QR코드

DOI QR Code

Effect of Different Level of Monensin Supplemented with Cold Process Urea Molasses Mineral Block on In vitro Rumen Fermentation at Different Days of Adaptation with Monensin

  • De, Debasis (lCAR Research Complex for NEH Region, Sikkim Centre) ;
  • Singh, G.P. (Division of Dairy Cattle Nutrition, National Dairy Research Institute)
  • Received : 2004.02.16
  • Accepted : 2004.07.22
  • Published : 2005.03.01

Abstract

Effect of period of adaptation and levels of monensin were studied for microbial fermentation/ digestibility to find out the optimum period of adaptation of monensin in rumen and suitable level of monensin in wheat straw+concentrate and wheat straw+UMMB diet. The mean digestibility of dry matter was decreased upto T-3 treatment (49.17%), however, digestibility of DM was affected upto period (P-2). NDF digestibility was affected due to treatment under P1 and P2 (p<0.05). Average digestibility of ADF was increased to 53.33% at T-3 level of monensin and P4 days of adaptation. TVFA (mmole/100 ml) were decreased from 9.49 in T-1 to 7.70 in T-7. Periods were not effectives except P2 (14 days of adaptation). Similarly, total gas was decrease with the increase of monensin levels in diet. Although acetate percentage in TVFA was not affected either due to level of monensin or period of adaptation but propionate was increased due to increase in monensin at 21 days of adaptation (P-3). Butyrate (%) was decreased significantly in T-2 to T-6 as compared to T-1 group. Total gas was significantly (p<0.01) higher in group T-1 (control) and it reduced significantly in T-5, however, differences in gas production between group T-3, T-5 and T-7 at P-1 was not significant. Methane production was reduced on P-3 and P-4 level of adaptation due to treatment. The overall result indicated that 21 days of adaptation with monensin was sufficient to mask the inhibiting effect of monensin to cell wall digestibility and 35 ppm monensin is optimum to reduce methane production and increase propionate productions.

Keywords

References

  1. Barnett, A. J. G. and R. L. Reid. 1957. Studies on the production of volatile fatty acid production from tiesh grass. J. Agric. Sci. Camb. 48:315.
  2. Beever, D. E., H. R. Losada, D. L. Gala, M. C. Spooner and M. S. Dhanoa. 1987. The use of monensin or formaldehyde to control the digestion of the nitrogenous constituents of perennial rye grass and white clover in the rumen of cattle. Br. J. Nutr. 57:57-67.
  3. Bergen, W. G. and D. B. Bates. 1984. Ionophores: Their effect on production efficiency and mode of action. J. Anim. Sci. 58:1465-1483.
  4. Boling, J. A., N. W. Bradley and L. D. Campbell. 1977. Monensin levels for grazing and finishing steers. J. Anim. Sci. 44:867-871.
  5. Chen, M. and M. J. Wolin. 1979. Effect of monensin and lasalocid-sodium on the growth of methanogenic and rumen Saccharolytic bacteria. Appl. Environ. Appl. Microbial. 38:72-77.
  6. Debasis De and G. P. Singh. 2003. Effect of ionophore enriched cold processed mineral block supplemented with urea molasses on rumen fermentation and microbial growth in cross bred cattle. Asian-Aust. J. Anim. Sci. 16:852-862.
  7. Dinius, D. A., H. K. Goering, R. R. Oltjen and H. R. Cross. 1978. Finishing beef steerson forage diets with additives and supplemental lipids. J. Anim. Sci. 46:761-768.
  8. Dawson, K. A. and J. A. Boling. 1983. Monensin-resistant bacteria in the rumens of calves on monensin containing and unmedicated diets. Appl. Environ. Microbial. 46:160-164.
  9. Erwin, E. S., G. A. Macro and E. M. Emery. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44:1768-1775.
  10. Goodrich, R. D., J. E. Garrett, D. R. Gast, M. A. Krick, D. A. Larson and J. C. Meiske. 1984. Influence of monensin on the performance of cattle. J. Anim. Sci. 58:1484.
  11. Haney, M. E. Jr. and Hoehn. 1968. Monensin, a new biologically active compound. I. Discovery and isolation. Antimicrob. Agents Chemother, 1967. pp. 349-352.
  12. Joblin, K. N. 1981. Isolation, enumeration and maintenance of rumen anaerobic fungi in rolled tubes. Appl. Environ Microbiol. 42:1119-1122.
  13. Kearl, C. L. 1982. Nutrients requirement of ruminants in developing countries. International Feedstuffs Institute. Utah Agricultural Experiment Station, Utah State University, Logan Utah.
  14. Mohini, M. 1991. Effect of urea molasses mineral block supplementation to straw based diets on fibre digestibility, rumen fermentation pattern and nutrient utilization and growth in buffalo calves, Ph.D Thesis, NDRI, Kamal.
  15. Mc Dougall, E. I. 1948. The composition and output of sheep's saliva. Biochem. J. 43:99.
  16. Menke, K. H., L. Raap, A., Salawsky, H. Steingaso, D. Fritz and W. Scheneider. 1979. The estimation of the digestibility and metabolisable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. J. Agric. Sci. Camb. 93:217-223.
  17. Nagaraja, T. G. and M. B. Taylor. 1987. Susceptibility and resistance of ruminal bacteria to antimicrobial feed additives. Appl. Environ. Microbiol. 53:1620-1625.
  18. Owaimer, A. N., M. S. Kraidees, M. Al. Saiady, S. Zahran and M. A. Abouheif. 2003. Effect of feeding monensin in combination with zeranol implants on performance, carcass trait and nutrient digestively of growing lambs. Asian-Aust. J. Anim. Sci. 16:1274-1279.
  19. Potter, E. L., A. P. Raun, C. O. Cooley, R. P. Rathmacher and L. F. Richardson. 1976. Effect of monensin on carcass characteristics, carcass composition and efficiency of converting feed to carcass. J. Anim. Sci. 43:678-683.
  20. Richardson, L. F., A. P. Raun, E. L. Potter, C. O. Cooley and R. P. Rathmacher. 1976. Effect of monensin on rumen fermentation in vitro and in vivo. J. Anim. Sci. 43:657-664.
  21. Simpson, M. E. 1978. Effects of certain antibiotics on in vitro cellulose digestibility and volatile fatty acid production by ruminal organisms. J. Anim. Sci. 47(suppl.1):439.
  22. Simpson, M. E. 1980. Effect of added antibiotics on in vitro rate and extent of digestion of a wheat straw cell wall. J. Anim. Sci. (suppl. 1):394.
  23. Singh, G. P. and M. Mohini. 1999. Effect of different levels of rumensin in diet on rumen fermentation, nutrient digestibility and methane production in cattle. Asian-Aust. J. Anim. Sci. 12:1215-1221.
  24. Snedecor, G. W. and W. G. Cochran. 1986. Statistical Methods. Oxford and IBH Publishing Co., Calcutta, India.
  25. Tilley, J. M. A. and R. A. Terry. 1963. A two stage technique for the in vitro digestion of forage crops. J. Br. Grassland Soc. 18:104-112.
  26. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Method of dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3582-3597.
  27. Wang, Y., T. A. Mcallister, J. Baah, R. Wilde, K. A. Beauchemin, L. M. Rode, J. A. Shelford, G. M. Kamande and K. J. Cheng. 2003. Effects of Tween 80 on In vitro fermentation of silages and interactive effects of tween 80, monensin and exogenous fibrolytic enzymes on growth performance by feedlot cattle. Asian-Aust. J. Anim. Sci. 16(7):986-978.
  28. Zinn, R. A., A. Plascencia and R. Barajas. 1994. Interaction of forage level and monensin in diets for feed lot cattle on growth performance and digestive function. J. Anim. Sci. 72(9):2209-2215.

Cited by

  1. Influence of Dietary Salinomycin on Feeding-induced Variations of Glucose Kinetics and Blood Volatile Fatty Acids and Insulin Concentrations in Sheep Fed a High-roughage Diet vol.20, pp.3, 2005, https://doi.org/10.5713/ajas.2007.365
  2. Effect of Disodium Fumarate on In vitro Rumen Fermentation of Different Substrates and Rumen Bacterial Communities as Revealed by Denaturing Gradient Gel Electrophoresis Analysis of 16S Ribosomal DNA vol.20, pp.4, 2005, https://doi.org/10.5713/ajas.2007.543
  3. Effect of Monensin and Live Yeast Supplementation on Growth Performance, Nutrient Digestibility, Carcass Characteristics and Ruminal Fermentation Parameters in Lambs Fed Steam-flaked Corn-based Diets vol.21, pp.4, 2005, https://doi.org/10.5713/ajas.2008.70353